ID   BP26_BRUA1              Reviewed;         250 AA.
AC   Q44642; B2S6U1;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   22-SEP-2009, sequence version 2.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=26 kDa periplasmic immunogenic protein;
DE   Flags: Precursor;
GN   Name=bp26; OrderedLocusNames=BAbS19_I13950;
OS   Brucella abortus (strain S19).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=430066;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 29-39, AND
RP   SUBCELLULAR LOCATION.
RX   PubMed=8748294; DOI=10.1128/jcm.34.1.165-169.1996;
RA   Rossetti O.L., Arese A.I., Boschiroli M.L., Cravero S.L.;
RT   "Cloning of Brucella abortus gene and characterization of expressed 26-
RT   kilodalton periplasmic protein: potential use for diagnosis.";
RL   J. Clin. Microbiol. 34:165-169(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=S19;
RX   PubMed=18478107; DOI=10.1371/journal.pone.0002193;
RA   Crasta O.R., Folkerts O., Fei Z., Mane S.P., Evans C., Martino-Catt S.,
RA   Bricker B., Yu G., Du L., Sobral B.W.;
RT   "Genome sequence of Brucella abortus vaccine strain S19 compared to
RT   virulent strains yields candidate virulence genes.";
RL   PLoS ONE 3:E2193-E2193(2008).
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000269|PubMed:8748294}.
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DR   EMBL; Z54148; CAA90862.1; -; Genomic_DNA.
DR   EMBL; CP000887; ACD72888.1; -; Genomic_DNA.
DR   RefSeq; WP_002969580.1; NC_010742.1.
DR   PDB; 4HVZ; X-ray; 3.50 A; A/B/C/D=29-250.
DR   PDBsum; 4HVZ; -.
DR   AlphaFoldDB; Q44642; -.
DR   SMR; Q44642; -.
DR   EnsemblBacteria; ACD72888; ACD72888; BAbS19_I13950.
DR   GeneID; 3788832; -.
DR   KEGG; bmc:BAbS19_I13950; -.
DR   HOGENOM; CLU_080344_4_0_5; -.
DR   OMA; LPNGPHI; -.
DR   Proteomes; UP000002565; Chromosome 1.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   InterPro; IPR007497; DUF541.
DR   Pfam; PF04402; SIMPL; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Periplasm; Signal.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000269|PubMed:8748294"
FT   CHAIN           29..250
FT                   /note="26 kDa periplasmic immunogenic protein"
FT                   /id="PRO_0000020825"
FT   CONFLICT        50
FT                   /note="A -> P (in Ref. 1; CAA90862)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        76
FT                   /note="N -> NNEAMTANN (in Ref. 1; CAA90862)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        80
FT                   /note="T -> R (in Ref. 1; CAA90862)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        145
FT                   /note="D -> H (in Ref. 1; CAA90862)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        158
FT                   /note="L -> F (in Ref. 1; CAA90862)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        170..173
FT                   /note="NEAR -> TRG (in Ref. 1; CAA90862)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        184
FT                   /note="A -> P (in Ref. 1; CAA90862)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        207
FT                   /note="P -> PPM (in Ref. 1; CAA90862)"
FT                   /evidence="ECO:0000305"
FT   STRAND          39..50
FT                   /evidence="ECO:0007829|PDB:4HVZ"
FT   STRAND          54..67
FT                   /evidence="ECO:0007829|PDB:4HVZ"
FT   HELIX           68..89
FT                   /evidence="ECO:0007829|PDB:4HVZ"
FT   STRAND          95..97
FT                   /evidence="ECO:0007829|PDB:4HVZ"
FT   STRAND          102..108
FT                   /evidence="ECO:0007829|PDB:4HVZ"
FT   STRAND          120..134
FT                   /evidence="ECO:0007829|PDB:4HVZ"
FT   HELIX           137..139
FT                   /evidence="ECO:0007829|PDB:4HVZ"
FT   HELIX           140..149
FT                   /evidence="ECO:0007829|PDB:4HVZ"
FT   HELIX           166..190
FT                   /evidence="ECO:0007829|PDB:4HVZ"
FT   STRAND          194..203
FT                   /evidence="ECO:0007829|PDB:4HVZ"
FT   HELIX           215..217
FT                   /evidence="ECO:0007829|PDB:4HVZ"
FT   HELIX           218..222
FT                   /evidence="ECO:0007829|PDB:4HVZ"
FT   STRAND          237..250
FT                   /evidence="ECO:0007829|PDB:4HVZ"
SQ   SEQUENCE   250 AA;  26553 MW;  00F948BF0CDB320F CRC64;
     MNTRASNFLA ASFSTIMLVG AFSLPAFAQE NQMTTQPARI AVTGEGMMTA SPDMAILNLS
     VLRQAKTARE AMTANNEAMT KVLDAMKKAG IEDRDLQTGG IDIQPIYVYP DDKNNLKEPT
     ITGYSVSTSL TVRVRELANV GKILDESVTL GVNQGGDLNL VNDNPSAVIN EARKRAVANA
     IAKAKTLADA AGVGLGRVVE ISELSRPPMP MPIARGQFRT MLAAAPDNSV PIAAGENSYN
     VSVNVVFEIK