T132E_MOUSE
ID T132E_MOUSE Reviewed; 1074 AA.
AC Q6IEE6; A0A0A0MQ93; B7ZN59; Q5SUX0;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 10-APR-2019, sequence version 2.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Transmembrane protein 132E;
DE Flags: Precursor;
GN Name=Tmem132e; Synonyms=Gm644;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2] {ECO:0000312|EMBL:AAI45027.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain {ECO:0000312|EMBL:AAI45027.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION.
RX PubMed=14569139; DOI=10.1136/jmg.40.10.e116;
RA Kehrer-Sawatzki H., Tinschert S., Jenne D.E.;
RT "Heterogeneity of breakpoints in non-LCR-mediated large constitutional
RT deletions of the 17q11.2 NF1 tumour suppressor region.";
RL J. Med. Genet. 40:E116-E116(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=25331638; DOI=10.1002/humu.22712;
RA Li J., Zhao X., Xin Q., Shan S., Jiang B., Jin Y., Yuan H., Dai P.,
RA Xiao R., Zhang Q., Xiao J., Shao C., Gong Y., Liu Q.;
RT "Whole-exome sequencing identifies a variant in TMEM132E causing autosomal-
RT recessive nonsyndromic hearing loss DFNB99.";
RL Hum. Mutat. 36:98-105(2015).
CC -!- FUNCTION: Required for normal inner ear hair cell function and hearing.
CC {ECO:0000250|UniProtKB:Q6IEE7}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Widely expressed, with highest levels in the
CC cochlea. In the cochlea, detected in spiral ganglion, the organ of
CC Corti and stria vascularis. In the organ of Corti, prominently
CC expressed in the outer and inner hair cells, especially at the apical
CC and basal region of the outer hair cell body (at protein level).
CC {ECO:0000269|PubMed:25331638}.
CC -!- SIMILARITY: Belongs to the TMEM132 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAD80243.1; Type=Miscellaneous discrepancy; Note=Artifactual sequence.; Evidence={ECO:0000305};
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DR EMBL; AL645797; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC137906; AAI37907.1; -; mRNA.
DR EMBL; BC145026; AAI45027.1; -; mRNA.
DR EMBL; BN000151; CAD80243.1; ALT_SEQ; mRNA.
DR CCDS; CCDS25144.2; -.
DR RefSeq; NP_001291368.1; NM_001304439.1.
DR AlphaFoldDB; Q6IEE6; -.
DR STRING; 10090.ENSMUSP00000052484; -.
DR GlyGen; Q6IEE6; 4 sites.
DR iPTMnet; Q6IEE6; -.
DR PhosphoSitePlus; Q6IEE6; -.
DR MaxQB; Q6IEE6; -.
DR PaxDb; Q6IEE6; -.
DR PRIDE; Q6IEE6; -.
DR ProteomicsDB; 254522; -.
DR ProteomicsDB; 322126; -.
DR Antibodypedia; 55084; 66 antibodies from 15 providers.
DR DNASU; 270893; -.
DR Ensembl; ENSMUST00000054245; ENSMUSP00000052484; ENSMUSG00000020701.
DR GeneID; 270893; -.
DR KEGG; mmu:270893; -.
DR UCSC; uc007kmv.2; mouse.
DR CTD; 124842; -.
DR MGI; MGI:2685490; Tmem132e.
DR VEuPathDB; HostDB:ENSMUSG00000020701; -.
DR eggNOG; KOG4789; Eukaryota.
DR GeneTree; ENSGT00940000158479; -.
DR HOGENOM; CLU_009871_0_0_1; -.
DR InParanoid; Q6IEE6; -.
DR OMA; QCKSTNE; -.
DR OrthoDB; 598074at2759; -.
DR TreeFam; TF314981; -.
DR BioGRID-ORCS; 270893; 0 hits in 58 CRISPR screens.
DR PRO; PR:Q6IEE6; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q6IEE6; protein.
DR Bgee; ENSMUSG00000020701; Expressed in lumbar dorsal root ganglion and 98 other tissues.
DR ExpressionAtlas; Q6IEE6; baseline and differential.
DR Genevisible; Q6IEE6; MM.
DR GO; GO:0044297; C:cell body; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0035677; P:posterior lateral line neuromast hair cell development; ISO:MGI.
DR InterPro; IPR026307; TMEM132.
DR InterPro; IPR031436; TMEM132_C.
DR InterPro; IPR031437; TMEM132_M.
DR InterPro; IPR031435; TMEM132_N.
DR PANTHER; PTHR13388; PTHR13388; 1.
DR Pfam; PF16070; TMEM132; 1.
DR Pfam; PF15706; TMEM132D_C; 1.
DR Pfam; PF15705; TMEM132D_N; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..1074
FT /note="Transmembrane protein 132E"
FT /id="PRO_0000287104"
FT TOPO_DOM 26..894
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 895..915
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 916..1074
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 205..224
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 243..266
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 564..587
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 816..867
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 962..1064
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 970..1003
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1010..1025
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1031..1046
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 70
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 91
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 320
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 401
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 256..257
FT /note="Missing (in Ref. 2; AAI37907/AAI45027)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1074 AA; 116235 MW; D734195FE447ED58 CRC64;
MAPGMSGRRG AALLCLSVLL AHAASRSHPA SPSPPGTQAS PILPVSYRLS HTRLAFFLKE
ARPLTPAAIN GSLQRSEPFV VFQTKELPVL NVSLGPFSTS QVVARELLQP SSTLDIPERL
TVNWKVRAFI VRARVPASQP VAQVLFYVAG RDWDDFGVTE RLPCVRLHAF RDAREVRSSC
RLGGALATCL VRAELPLAWF GPPAPAAPPS ARRKSPDGLE PEAAAESQQV ELYYTLHAPD
ASGGCGSARR GPGPGPGAAA RAESPTQHPL LRIGSISLFR PPPRRAVQEH RLDSNLMIRL
PDRPLRPGEV LSILLYLAPN SSSAASPSVE HFTLRVKAKK GVTLLGTKSR SGQWRVTSEL
LTGAKHSTAT VDVAWALDTP LPPWEGQGPL EILQLDFEME NFTSQSVKRR IMWHIDYRGH
SALPDLERAV TELTVIQRDV QAILPLAMDT EIINTAILTG RTVAIPVKVI AIEVTGLVLD
VSDLVECQSH SEDIIKVSSS CDYVFVSGKE SRGSMNARVT FRYDVLSAPL EMTVWVPKLP
LHIELSDARL SQVKGWRVPI LPDRRSARES EDEEEEEEER RQSANRGCTL QYQHATLQVF
TQFHTTSSEG TDQVVTMLGP DWLVEVTDLV SDFMRVGDPR VAHLVDSNTL AGLEPGTTPF
KVVSPLTEAV LGETLLTVTE EKVSITQLQA QVVASLTLSL RPSPGSSHTI LATTAAQPTL
SLVKQEALLS LWLSYSDGTT APLSLYSSRD YGLLVSSLDE RVATVTQDKA FPLVVAEAEG
SGDLLRAELT ISESCQKTKR KSVLATTPVS LRVHFGRDEE DPTYDYPGPS QPGPGGGEDE
ARGAGPPGTA IPAGEVPGLG TAGPVPPTED YLPLPTGFLQ MPRGLTDLEI GMYALLGVFC
LAILVFLINC IVFVLRYRHK RIPPEGQTSM DHSHHWVFLG NGQPLRVQGE LSPPAGSALE
TVPACCHGDH HSSGSSQTSV QSQVHGRGDG SSGGSARDQT EDPASSPTSK RKRVKFTTFT
TLPTEELAYD SVPAGEEEDE EEDLGWGCPD VAGTTRPTPP PDLHNYMRRI KDIA
