ID   T1353_SHIFL             Reviewed;         514 AA.
AC   Q79CE8;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Probable transposase for insertion sequence element IS1353;
OS   Shigella flexneri.
OG   Plasmid IncFII R100 (NR1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=IncFII R100 (NR1);
RX   PubMed=8755869; DOI=10.1128/jb.178.15.4429-4437.1996;
RA   Brown H.J., Stokes H.W., Hall R.M.;
RT   "The integrons In0, In2, and In5 are defective transposon derivatives.";
RL   J. Bacteriol. 178:4429-4437(1996).
RN   [2]
RP   SEQUENCE REVISION TO 211-223.
RC   PLASMID=IncFII R100 (NR1);
RX   PubMed=12499211; DOI=10.1128/aac.47.1.342-349.2003;
RA   Partridge S.R., Hall R.M.;
RT   "In34, a complex In5 family class 1 integron containing orf513 and
RT   dfrA10.";
RL   Antimicrob. Agents Chemother. 47:342-349(2003).
CC   -!- FUNCTION: Probably involved in the transposition of insertion sequence
CC       IS1353.
CC   -!- MISCELLANEOUS: IS1353 is an insertion seqeunce sometimes found in In2.
CC   -!- SIMILARITY: Belongs to the transposase 8 family. {ECO:0000305}.
CC   -!- CAUTION: A similar protein in IS911 has been shown to only be produced
CC       upon ribosomal frameshifting. This does not seem to be the case for
CC       this protein, as DNA sequencing shows one complete open reading frame.
CC       {ECO:0000305}.
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DR   EMBL; U40482; AAC44291.2; -; Genomic_DNA.
DR   EMBL; U42226; AAC53730.2; -; Genomic_DNA.
DR   RefSeq; WP_000287615.1; NZ_UDTD01000066.1.
DR   AlphaFoldDB; Q79CE8; -.
DR   PRIDE; Q79CE8; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004803; F:transposase activity; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006313; P:transposition, DNA-mediated; IEA:InterPro.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR025948; HTH-like_dom.
DR   InterPro; IPR001584; Integrase_cat-core.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   InterPro; IPR002514; Transposase_8.
DR   Pfam; PF13276; HTH_21; 1.
DR   Pfam; PF01527; HTH_Tnp_1; 1.
DR   Pfam; PF00665; rve; 1.
DR   Pfam; PF13333; rve_2; 1.
DR   SUPFAM; SSF46689; SSF46689; 2.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS50994; INTEGRASE; 1.
PE   3: Inferred from homology;
KW   Coiled coil; DNA integration; DNA recombination; Endonuclease; Hydrolase;
KW   Magnesium; Metal-binding; Nuclease; Plasmid.
FT   CHAIN           1..514
FT                   /note="Probable transposase for insertion sequence element
FT                   IS1353"
FT                   /id="PRO_0000284142"
FT   DOMAIN          346..510
FT                   /note="Integrase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00457"
FT   COILED          172..216
FT                   /evidence="ECO:0000255"
FT   BINDING         357
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00457"
FT   BINDING         417
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00457"
SQ   SEQUENCE   514 AA;  59588 MW;  9984076FB09FE9CA CRC64;
     MYSYEDRLRA VRLYLKLGRR MSATLRQLGY PTKNSLKAWL AEFERNQDLR RGYQRIKRQY
     TDEQKQRAVD HYIEQGYCLS HTIRSLGYPS REALRAWIRD LRPEFARTVV GSSAPTVARS
     RLEKQQAVIA LNLRVGSAKD VADTVGVSRP TLYNWQHRLL GKVPLKPMTK KKGDTSLEQR
     HEALLRELAE LESQNQRLRM ENAILEKASE LIKKDMGINP LELTSREKTK VVDALRVTFP
     LANLLCGLKL ARSTYFYQRL RQTRPDKYTQ VREVIRTIFE DNYRCYGYRR IDSALRLGGM
     RVSEKVVRRL MAQERLVVRT PRRRRFSAYA GDPTPAVPNL LNRDFHASAP NTKWLTDLTE
     IHIPAGKVYV SPIVDCFDGL VVAWNIGTSP DANLVNTMLD HAVRTLRPGE HPVIHSDRGS
     HYRWPAWIRR TENAQLTRSM SKKGCSPDNA ACEGFFGRLK TELIYPRNWQ HVTLKDLMTR
     IDAYIHWYNE RRIKVSLGGR SPIEYRHAVG LMSV