ID   T150B_DANRE             Reviewed;         232 AA.
AC   Q32PK2;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Modulator of macroautophagy TMEM150B {ECO:0000305};
DE   AltName: Full=Transmembrane protein 150B {ECO:0000250|UniProtKB:A6NC51};
GN   Name=tmem150b {ECO:0000250|UniProtKB:A6NC51}; ORFNames=zgc:123244;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Larva;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Modulator of macroautophagy that causes accumulation of
CC       autophagosomes under basal conditions and enhances autophagic flux (By
CC       similarity). Represses cell death and promotes long-term clonogenic
CC       survival of cells grown in the absence of glucose in a macroautophagy-
CC       independent manner (By similarity). May have some role in extracellular
CC       matrix engulfment or growth factor receptor recycling, both of which
CC       can modulate cell survival (By similarity).
CC       {ECO:0000250|UniProtKB:A6NC51}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:A6NC51};
CC       Multi-pass membrane protein {ECO:0000250|UniProtKB:A6NC51}. Endosome
CC       membrane {ECO:0000250|UniProtKB:A6NC51}; Multi-pass membrane protein
CC       {ECO:0000255}. Cytoplasmic vesicle, autophagosome membrane
CC       {ECO:0000250|UniProtKB:A6NC51}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the DRAM/TMEM150 family. {ECO:0000305}.
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DR   EMBL; BC108083; AAI08084.1; -; mRNA.
DR   RefSeq; NP_001032458.1; NM_001037381.1.
DR   AlphaFoldDB; Q32PK2; -.
DR   STRING; 7955.ENSDARP00000073041; -.
DR   PaxDb; Q32PK2; -.
DR   GeneID; 556933; -.
DR   KEGG; dre:556933; -.
DR   CTD; 284417; -.
DR   ZFIN; ZDB-GENE-051113-144; tmem150b.
DR   eggNOG; KOG4320; Eukaryota.
DR   InParanoid; Q32PK2; -.
DR   OrthoDB; 1199230at2759; -.
DR   PhylomeDB; Q32PK2; -.
DR   PRO; PR:Q32PK2; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0000421; C:autophagosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005887; C:integral component of plasma membrane; ISS:UniProtKB.
DR   GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR   InterPro; IPR019402; Frag1/DRAM/Sfk1.
DR   Pfam; PF10277; Frag1; 1.
PE   2: Evidence at transcript level;
KW   Autophagy; Cell membrane; Cytoplasmic vesicle; Endosome; Glycoprotein;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..232
FT                   /note="Modulator of macroautophagy TMEM150B"
FT                   /id="PRO_0000349286"
FT   TOPO_DOM        1..6
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        28..50
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        51..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        72..83
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        84..104
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        105..115
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        116..136
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        137..153
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        154..174
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        175..177
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        178..198
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        199..232
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q86TG1"
FT   CARBOHYD        29
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        33
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   232 AA;  26016 MW;  639C78E6DF2A5B2A CRC64;
     MWAWALLPVF LAVFGTVGLW AVYAIAVSNN SVNITIEFPY ISTCGAYTPQ SCLFAQICNI
     CCVLALWIVV IRFQQIRDLG RSSHLNTAGL VLGFISSIGI SILGNFQQTI IQEVHLLGAL
     MAFFLGLAYF WIQAFITYFS PPSRDNKWLV PVRFVLCSQC TCMVICMFVL HSTGFRSAAA
     ICEWILVMCF FALFGVFAAE FRHIDFHKLT VQKEGLKVAN NDNVVWTVQD VQ