T150B_MOUSE
ID T150B_MOUSE Reviewed; 238 AA.
AC Q8R218; Q8C9L9;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Modulator of macroautophagy TMEM150B {ECO:0000305};
DE AltName: Full=Transmembrane protein 150B {ECO:0000250|UniProtKB:A6NC51};
GN Name=Tmem150b {ECO:0000250|UniProtKB:A6NC51};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Modulator of macroautophagy that causes accumulation of
CC autophagosomes under basal conditions and enhances autophagic flux (By
CC similarity). Represses cell death and promotes long-term clonogenic
CC survival of cells grown in the absence of glucose in a macroautophagy-
CC independent manner (By similarity). May have some role in extracellular
CC matrix engulfment or growth factor receptor recycling, both of which
CC can modulate cell survival (By similarity).
CC {ECO:0000250|UniProtKB:A6NC51}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:A6NC51};
CC Multi-pass membrane protein {ECO:0000250|UniProtKB:A6NC51}. Endosome
CC membrane {ECO:0000250|UniProtKB:A6NC51}; Multi-pass membrane protein
CC {ECO:0000255}. Cytoplasmic vesicle, autophagosome membrane
CC {ECO:0000250|UniProtKB:A6NC51}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Localizes mainly at the plasma membrane where it
CC concentrates at actin-rich focal adhesions (By similarity).
CC {ECO:0000250|UniProtKB:A6NC51}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8R218-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8R218-2; Sequence=VSP_035310, VSP_035311;
CC -!- SIMILARITY: Belongs to the DRAM/TMEM150 family. {ECO:0000305}.
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DR EMBL; AK041846; BAC31082.1; -; mRNA.
DR EMBL; BC022651; AAH22651.1; -; mRNA.
DR CCDS; CCDS20742.1; -. [Q8R218-2]
DR CCDS; CCDS51975.1; -. [Q8R218-1]
DR RefSeq; NP_001136264.1; NM_001142792.1. [Q8R218-1]
DR RefSeq; NP_808555.1; NM_177887.4. [Q8R218-2]
DR AlphaFoldDB; Q8R218; -.
DR STRING; 10090.ENSMUSP00000083549; -.
DR GlyGen; Q8R218; 1 site.
DR PaxDb; Q8R218; -.
DR PRIDE; Q8R218; -.
DR Antibodypedia; 66997; 54 antibodies from 11 providers.
DR Ensembl; ENSMUST00000086363; ENSMUSP00000083549; ENSMUSG00000046456. [Q8R218-1]
DR Ensembl; ENSMUST00000086364; ENSMUSP00000083550; ENSMUSG00000046456. [Q8R218-2]
DR GeneID; 330460; -.
DR KEGG; mmu:330460; -.
DR UCSC; uc009eyg.2; mouse. [Q8R218-2]
DR UCSC; uc009eyh.2; mouse. [Q8R218-1]
DR CTD; 284417; -.
DR MGI; MGI:2679718; Tmem150b.
DR VEuPathDB; HostDB:ENSMUSG00000046456; -.
DR eggNOG; KOG4320; Eukaryota.
DR GeneTree; ENSGT01030000234578; -.
DR HOGENOM; CLU_059992_3_0_1; -.
DR InParanoid; Q8R218; -.
DR OMA; WICIIRY; -.
DR OrthoDB; 1199230at2759; -.
DR PhylomeDB; Q8R218; -.
DR TreeFam; TF314508; -.
DR BioGRID-ORCS; 330460; 1 hit in 73 CRISPR screens.
DR PRO; PR:Q8R218; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q8R218; protein.
DR Bgee; ENSMUSG00000046456; Expressed in small intestine Peyer's patch and 41 other tissues.
DR Genevisible; Q8R218; MM.
DR GO; GO:0000421; C:autophagosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:UniProtKB.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR InterPro; IPR019402; Frag1/DRAM/Sfk1.
DR Pfam; PF10277; Frag1; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Autophagy; Cell membrane; Cytoplasmic vesicle;
KW Endosome; Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..238
FT /note="Modulator of macroautophagy TMEM150B"
FT /id="PRO_0000349285"
FT TOPO_DOM 1..8
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..51
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 73..86
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 108..116
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 138..156
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 157..177
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 178..186
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 208..238
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q86TG1"
FT CARBOHYD 30
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 109..123
FT /note="DKNQKPTHLAGAFLA -> RSPIPQSWSRETHRR (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_035310"
FT VAR_SEQ 124..238
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_035311"
SQ SEQUENCE 238 AA; 26578 MW; E38497FCA7BFCFB5 CRC64;
MWNYLSLLPV ILFLWAIAGI WIVFAIAVVN GSVDLNEGFP FISICGSYAP QSCIFGQVLN
IGAALTVWIC IVRHHQLRDW GVKTWQNQLI LWSGILCALG TSIVGNFQDK NQKPTHLAGA
FLAFILGNLY FWLQFFLSWW VKGLPQPGPH WIKSLRLSLC SLSTILIVAM IVLHALHMRS
ASAICEWVVA MLLFMLFGFF AVDFSILRGC TLHLHPRLDS SLPQAPSGSP NIQMAQVL
