T161B_MOUSE
ID T161B_MOUSE Reviewed; 487 AA.
AC Q8C2L6; Q8BT72;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Transmembrane protein 161B;
GN Name=Tmem161b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Embryo, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=33597309; DOI=10.1073/pnas.2018220118;
RA Koopman C.D., De Angelis J., Iyer S.P., Verkerk A.O., Da Silva J.,
RA Berecki G., Jeanes A., Baillie G.J., Paterson S., Uribe V., Ehrlich O.V.,
RA Robinson S.D., Garric L., Petrou S., Simons C., Vetter I., Hogan B.M.,
RA de Boer T.P., Bakkers J., Smith K.A.;
RT "The zebrafish grime mutant uncovers an evolutionarily conserved role for
RT Tmem161b in the control of cardiac rhythm.";
RL Proc. Natl. Acad. Sci. U.S.A. 118:0-0(2021).
CC -!- FUNCTION: Essential for maintaining normal cardiac rhythm in the
CC developing heart and for neonatal survival (PubMed:33597309). Inhibits
CC potassium and calcium currents in the cardiomyocytes, this assists in
CC timely action potential repolarization and thereby maintains normal
CC cardiac rhythm (PubMed:33597309). {ECO:0000269|PubMed:33597309}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q7SY10};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Knockout mice are perinatal lethal and isolated
CC embryonic cardiomyocytes exhibit arrhythmic calcium oscillations.
CC {ECO:0000269|PubMed:33597309}.
CC -!- SIMILARITY: Belongs to the TMEM161 family. {ECO:0000305}.
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DR EMBL; AK088393; BAC40324.1; -; mRNA.
DR EMBL; AK013305; BAC25401.1; -; mRNA.
DR EMBL; BC051663; AAH51663.1; -; mRNA.
DR CCDS; CCDS36739.1; -.
DR RefSeq; NP_780396.2; NM_175187.5.
DR RefSeq; XP_006517466.1; XM_006517403.3.
DR RefSeq; XP_006517467.1; XM_006517404.3.
DR RefSeq; XP_006517468.1; XM_006517405.3.
DR RefSeq; XP_011242867.1; XM_011244565.2.
DR AlphaFoldDB; Q8C2L6; -.
DR STRING; 10090.ENSMUSP00000055208; -.
DR GlyGen; Q8C2L6; 3 sites.
DR PhosphoSitePlus; Q8C2L6; -.
DR EPD; Q8C2L6; -.
DR MaxQB; Q8C2L6; -.
DR PaxDb; Q8C2L6; -.
DR PRIDE; Q8C2L6; -.
DR ProteomicsDB; 263220; -.
DR Antibodypedia; 50472; 75 antibodies from 15 providers.
DR DNASU; 72745; -.
DR Ensembl; ENSMUST00000057495; ENSMUSP00000055208; ENSMUSG00000035762.
DR GeneID; 72745; -.
DR KEGG; mmu:72745; -.
DR UCSC; uc007rip.2; mouse.
DR CTD; 153396; -.
DR MGI; MGI:1919995; Tmem161b.
DR VEuPathDB; HostDB:ENSMUSG00000035762; -.
DR eggNOG; KOG3978; Eukaryota.
DR GeneTree; ENSGT00390000000672; -.
DR HOGENOM; CLU_027277_0_0_1; -.
DR InParanoid; Q8C2L6; -.
DR OMA; RFALMPI; -.
DR OrthoDB; 734854at2759; -.
DR PhylomeDB; Q8C2L6; -.
DR TreeFam; TF314570; -.
DR BioGRID-ORCS; 72745; 4 hits in 74 CRISPR screens.
DR ChiTaRS; Tmem161b; mouse.
DR PRO; PR:Q8C2L6; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q8C2L6; protein.
DR Bgee; ENSMUSG00000035762; Expressed in spermatocyte and 232 other tissues.
DR ExpressionAtlas; Q8C2L6; baseline and differential.
DR Genevisible; Q8C2L6; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0098901; P:regulation of cardiac muscle cell action potential; IMP:UniProtKB.
DR GO; GO:0002027; P:regulation of heart rate; IMP:UniProtKB.
DR InterPro; IPR019395; Transmembrane_161A/B.
DR PANTHER; PTHR13624; PTHR13624; 1.
DR Pfam; PF10268; Tmemb_161AB; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..487
FT /note="Transmembrane protein 161B"
FT /id="PRO_0000288090"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 367..387
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 459..479
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 34
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 135
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 203
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 487 AA; 55467 MW; 7948B37ACDAE6EFA CRC64;
MGVIGIQLVV TMVMASVMQK IIPHYSLARW LLCNGSLRWY QHPSEEELRI LAGKQQKGKS
KKDRKYNGHI ENKPLTIPKD IDLHLETKSV TEVDTLALHY FPEYQWLVDF TVAATIVYLV
TEVYYSFMKP TQEMNISLVW CLLVLSFAIK VLFSLTTHYF KVEDGGERSV CVTFGFFFFV
KAMAVLIVTE NYLEFGLETG FTNFSDSAMQ FLEKQGLESQ GPVSKLTFKF FLAVFCSLIG
AFLTFPGLRL AQMHLDALNM ATEKITQTLL HINFLAPLFM VLLWVKPITK DYIMNPPLGR
ESVPLMTEAT FDTLRLWLII LLCVLRLAMM RSHLQAYLNL AQKCVDQMKK EAGRISTVEL
QKMVARVFYY LCVIALQYVA PLVMLLHMTL LLKTLGNHSW GIYPEAAFPL PVDNNLPANS
AYPELPSPDG KMKVTVTQIT VALSSLKNIF TPLLFRGLLS FLTWWIAACL FSTSLFGLFY
HQYLTVA
