T176B_HUMAN
ID T176B_HUMAN Reviewed; 270 AA.
AC Q3YBM2; B2RDK2; D3DWZ7; E9PAV4; Q5BJI2; Q9BT42; Q9Y609;
DT 06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Transmembrane protein 176B;
DE AltName: Full=Protein LR8;
GN Name=TMEM176B; Synonyms=LR8;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INDUCTION, AND
RP VARIANTS SER-55 AND ARG-94.
RC TISSUE=Lung;
RX PubMed=9922225; DOI=10.1165/ajrcmb.20.2.3368;
RA Lurton J., Rose T.M., Raghu G., Narayanan A.S.;
RT "Isolation of a gene product expressed by a subpopulation of human lung
RT fibroblasts by differential display.";
RL Am. J. Respir. Cell Mol. Biol. 20:327-331(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND VARIANT ARG-94.
RC TISSUE=Brain;
RA Anton B., Matus M., Canseco J., Calva J.C., Flores A., Gonzaga R.,
RA Salazar A., Arreola R., Leff P.;
RT "Cloning of a full-length Homo sapiens mRNA coding for a tetraspanin-like
RT LR8 protein.";
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Macrophage, and Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANTS ARG-94 AND
RP THR-134.
RC TISSUE=Lung, and Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP INDUCTION.
RX PubMed=16095493; DOI=10.1111/j.1600-6143.2005.01007.x;
RA Louvet C., Chiffoleau E., Heslan M., Tesson L., Heslan J.-M., Brion R.,
RA Beriou G., Guillonneau C., Khalife J., Anegon I., Cuturi M.-C.;
RT "Identification of a new member of the CD20/FcepsilonRIbeta family
RT overexpressed in tolerated allografts.";
RL Am. J. Transplant. 5:2143-2153(2005).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-236; SER-245 AND SER-258, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- FUNCTION: May play a role in the process of maturation of dendritic
CC cells. Required for the development of cerebellar granule cells (By
CC similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC Q3YBM2; Q92993: KAT5; NbExp=3; IntAct=EBI-2821479, EBI-399080;
CC Q3YBM2; P13473-2: LAMP2; NbExp=3; IntAct=EBI-2821479, EBI-21591415;
CC Q3YBM2; Q8TAP4-4: LMO3; NbExp=3; IntAct=EBI-2821479, EBI-11742507;
CC Q3YBM2; P17252: PRKCA; NbExp=3; IntAct=EBI-2821479, EBI-1383528;
CC Q3YBM2; Q15047-2: SETDB1; NbExp=3; IntAct=EBI-2821479, EBI-9090795;
CC Q3YBM2; Q96HP8: TMEM176A; NbExp=3; IntAct=EBI-2821479, EBI-2800645;
CC Q3YBM2; P61981: YWHAG; NbExp=3; IntAct=EBI-2821479, EBI-359832;
CC -!- SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3YBM2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3YBM2-2; Sequence=VSP_046283;
CC -!- TISSUE SPECIFICITY: Expressed in lung and dermal fibroblasts.
CC {ECO:0000269|PubMed:9922225}.
CC -!- INDUCTION: Up-regulated in fibrotic lung. Down-regulated in activated
CC dendritic cells. {ECO:0000269|PubMed:16095493,
CC ECO:0000269|PubMed:9922225}.
CC -!- SIMILARITY: Belongs to the TMEM176 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH91471.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF115384; AAD23440.1; -; mRNA.
DR EMBL; DQ144974; AAZ75648.1; -; mRNA.
DR EMBL; AK129525; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK315575; BAG37949.1; -; mRNA.
DR EMBL; AC006479; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471173; EAW54077.1; -; Genomic_DNA.
DR EMBL; CH471173; EAW54078.1; -; Genomic_DNA.
DR EMBL; CH471173; EAW54079.1; -; Genomic_DNA.
DR EMBL; CH471173; EAW54080.1; -; Genomic_DNA.
DR EMBL; BC004358; AAH04358.1; -; mRNA.
DR EMBL; BC014586; AAH14586.1; -; mRNA.
DR EMBL; BC091471; AAH91471.1; ALT_SEQ; mRNA.
DR CCDS; CCDS47746.1; -. [Q3YBM2-2]
DR CCDS; CCDS5908.1; -. [Q3YBM2-1]
DR RefSeq; NP_001094781.1; NM_001101311.1. [Q3YBM2-1]
DR RefSeq; NP_001094782.1; NM_001101312.1. [Q3YBM2-1]
DR RefSeq; NP_001094784.1; NM_001101314.1. [Q3YBM2-2]
DR RefSeq; NP_054739.3; NM_014020.3. [Q3YBM2-1]
DR RefSeq; XP_006715996.2; XM_006715933.3. [Q3YBM2-2]
DR AlphaFoldDB; Q3YBM2; -.
DR BioGRID; 118786; 28.
DR IntAct; Q3YBM2; 8.
DR STRING; 9606.ENSP00000410269; -.
DR TCDB; 1.A.37.8.1; the cd20 ca(2+) channel (cd20) family.
DR iPTMnet; Q3YBM2; -.
DR PhosphoSitePlus; Q3YBM2; -.
DR BioMuta; TMEM176B; -.
DR DMDM; 313104018; -.
DR jPOST; Q3YBM2; -.
DR MassIVE; Q3YBM2; -.
DR PaxDb; Q3YBM2; -.
DR PeptideAtlas; Q3YBM2; -.
DR PRIDE; Q3YBM2; -.
DR ProteomicsDB; 19087; -.
DR ProteomicsDB; 61891; -. [Q3YBM2-1]
DR Antibodypedia; 32900; 101 antibodies from 18 providers.
DR DNASU; 28959; -.
DR Ensembl; ENST00000326442.10; ENSP00000318409.5; ENSG00000106565.18. [Q3YBM2-1]
DR Ensembl; ENST00000429904.6; ENSP00000397810.2; ENSG00000106565.18. [Q3YBM2-1]
DR Ensembl; ENST00000434545.5; ENSP00000413531.1; ENSG00000106565.18. [Q3YBM2-1]
DR Ensembl; ENST00000447204.6; ENSP00000410269.2; ENSG00000106565.18. [Q3YBM2-1]
DR Ensembl; ENST00000450753.2; ENSP00000404831.2; ENSG00000106565.18. [Q3YBM2-2]
DR Ensembl; ENST00000492607.5; ENSP00000419258.1; ENSG00000106565.18. [Q3YBM2-1]
DR GeneID; 28959; -.
DR KEGG; hsa:28959; -.
DR MANE-Select; ENST00000326442.10; ENSP00000318409.5; NM_001101312.2; NP_001094782.1.
DR UCSC; uc003whu.5; human. [Q3YBM2-1]
DR CTD; 28959; -.
DR DisGeNET; 28959; -.
DR GeneCards; TMEM176B; -.
DR HGNC; HGNC:29596; TMEM176B.
DR HPA; ENSG00000106565; Tissue enriched (liver).
DR MIM; 610385; gene.
DR neXtProt; NX_Q3YBM2; -.
DR OpenTargets; ENSG00000106565; -.
DR PharmGKB; PA162405966; -.
DR VEuPathDB; HostDB:ENSG00000106565; -.
DR eggNOG; ENOG502SF8T; Eukaryota.
DR GeneTree; ENSGT00530000064074; -.
DR HOGENOM; CLU_090530_0_0_1; -.
DR InParanoid; Q3YBM2; -.
DR OMA; WEEDRCR; -.
DR OrthoDB; 1300114at2759; -.
DR PhylomeDB; Q3YBM2; -.
DR TreeFam; TF335389; -.
DR PathwayCommons; Q3YBM2; -.
DR SignaLink; Q3YBM2; -.
DR BioGRID-ORCS; 28959; 13 hits in 1071 CRISPR screens.
DR ChiTaRS; TMEM176B; human.
DR GenomeRNAi; 28959; -.
DR Pharos; Q3YBM2; Tbio.
DR PRO; PR:Q3YBM2; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q3YBM2; protein.
DR Bgee; ENSG00000106565; Expressed in right lobe of liver and 174 other tissues.
DR ExpressionAtlas; Q3YBM2; baseline and differential.
DR Genevisible; Q3YBM2; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009887; P:animal organ morphogenesis; TAS:ProtInc.
DR GO; GO:0097028; P:dendritic cell differentiation; IEA:Ensembl.
DR GO; GO:2001199; P:negative regulation of dendritic cell differentiation; IBA:GO_Central.
DR InterPro; IPR007237; CD20-like.
DR InterPro; IPR009281; TMEM176A/TMEM176B.
DR PANTHER; PTHR15756; PTHR15756; 1.
DR Pfam; PF04103; CD20; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Differentiation; Membrane; Nucleus; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..270
FT /note="Transmembrane protein 176B"
FT /id="PRO_0000279875"
FT TRANSMEM 65..85
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 237..270
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 256..270
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 236
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 245
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 254
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q925D4"
FT MOD_RES 258
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT VAR_SEQ 69..105
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_046283"
FT VARIANT 40
FT /note="G -> E (in dbSNP:rs10240587)"
FT /id="VAR_057766"
FT VARIANT 55
FT /note="P -> S (in dbSNP:rs11546671)"
FT /evidence="ECO:0000269|PubMed:9922225"
FT /id="VAR_031035"
FT VARIANT 70
FT /note="T -> A (in dbSNP:rs28434777)"
FT /id="VAR_031036"
FT VARIANT 94
FT /note="S -> R (in dbSNP:rs3173833)"
FT /evidence="ECO:0000269|PubMed:15489334,
FT ECO:0000269|PubMed:9922225, ECO:0000269|Ref.2"
FT /id="VAR_031037"
FT VARIANT 134
FT /note="A -> T (in dbSNP:rs2072443)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_031038"
FT VARIANT 180
FT /note="R -> W (in dbSNP:rs17256042)"
FT /id="VAR_031039"
FT CONFLICT 17..18
FT /note="PS -> HA (in Ref. 1; AAD23440)"
FT /evidence="ECO:0000305"
FT CONFLICT 237
FT /note="S -> P (in Ref. 3; AK129525)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 270 AA; 29056 MW; 6D27B2A384709FDD CRC64;
MTQNTVIVNG VAMASRPSQP THVNVHIHQE SALTQLLKAG GSLKKFLFHP GDTVPSTARI
GYEQLALGVT QILLGVVSCV LGVCLSLGPW TVLSASGCAF WAGSVVIAAG AGAIVHEKHP
GKLAGYISSL LTLAGFATAM AAVVLCVNSF IWQTEPFLYI DTVCDRSDPV FPTTGYRWMR
RSQENQWQKE ECRAYMQMLR KLFTAIRALF LAVCVLKVIV SLVSLGVGLR NLCGQSSQPL
NEEGSEKRLL GENSVPPSPS REQTSTAIVL
