T176B_MOUSE
ID T176B_MOUSE Reviewed; 263 AA.
AC Q9R1Q6; Q9WU52;
DT 06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Transmembrane protein 176B;
DE AltName: Full=Protein LR8;
GN Name=Tmem176b; Synonyms=Clast1, Lr8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Lung;
RX PubMed=9922225; DOI=10.1165/ajrcmb.20.2.3368;
RA Lurton J., Rose T.M., Raghu G., Narayanan A.S.;
RT "Isolation of a gene product expressed by a subpopulation of human lung
RT fibroblasts by differential display.";
RL Am. J. Respir. Cell Mol. Biol. 20:327-331(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND DISRUPTION PHENOTYPE.
RC TISSUE=B-cell;
RX PubMed=16814752; DOI=10.1016/j.brainres.2006.05.068;
RA Maeda Y., Fujimura L., O-Wang J., Hatano M., Sakamoto A., Arima M.,
RA Ebara M., Ino H., Yamashita T., Saisho H., Tokuhisa T.;
RT "Role of Clast1 in development of cerebellar granule cells.";
RL Brain Res. 1104:18-26(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Pancreas, and Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J.,
RA Wiemann S., Schick M., Korn B.;
RT "Cloning of mouse full open reading frames in Gateway(R) system entry
RT vector (pDONR201).";
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Kidney, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-253, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Liver, Lung, and Pancreas;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May play a role in the process of maturation of dendritic
CC cells (By similarity). Required for the development of cerebellar
CC granule cells. {ECO:0000250, ECO:0000269|PubMed:16814752}.
CC -!- SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed with higher expression in
CC lung, liver, kidney and colon. Expressed in cerebellar granule cells.
CC {ECO:0000269|PubMed:16814752}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the developing cerebellum at constant
CC levels from 18 dpc to P7. {ECO:0000269|PubMed:16814752}.
CC -!- DISRUPTION PHENOTYPE: Mice exhibit abnormal development of the
CC cerebellum and severe ataxia. {ECO:0000269|PubMed:16814752}.
CC -!- SIMILARITY: Belongs to the TMEM176 family. {ECO:0000305}.
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DR EMBL; AF115426; AAD28721.1; -; mRNA.
DR EMBL; AB031386; BAA83596.1; -; mRNA.
DR EMBL; AK007408; BAB25020.1; -; mRNA.
DR EMBL; AK156042; BAE33560.1; -; mRNA.
DR EMBL; CT010176; CAJ18384.1; -; mRNA.
DR EMBL; BC019539; AAH19539.1; -; mRNA.
DR EMBL; BC022166; AAH22166.1; -; mRNA.
DR CCDS; CCDS20116.1; -.
DR RefSeq; NP_001157679.1; NM_001164207.1.
DR RefSeq; NP_001157680.1; NM_001164208.1.
DR RefSeq; NP_001157681.1; NM_001164209.2.
DR RefSeq; NP_001273580.1; NM_001286651.1.
DR RefSeq; NP_001273581.1; NM_001286652.1.
DR RefSeq; NP_075543.1; NM_023056.4.
DR RefSeq; XP_006506519.1; XM_006506456.3.
DR RefSeq; XP_006506520.1; XM_006506457.3.
DR RefSeq; XP_006506521.1; XM_006506458.3.
DR RefSeq; XP_006506522.1; XM_006506459.3.
DR RefSeq; XP_006506523.1; XM_006506460.3.
DR RefSeq; XP_006506524.1; XM_006506461.3.
DR RefSeq; XP_017177199.1; XM_017321710.1.
DR AlphaFoldDB; Q9R1Q6; -.
DR BioGRID; 211160; 1.
DR STRING; 10090.ENSMUSP00000128705; -.
DR iPTMnet; Q9R1Q6; -.
DR PhosphoSitePlus; Q9R1Q6; -.
DR SwissPalm; Q9R1Q6; -.
DR EPD; Q9R1Q6; -.
DR jPOST; Q9R1Q6; -.
DR MaxQB; Q9R1Q6; -.
DR PaxDb; Q9R1Q6; -.
DR PeptideAtlas; Q9R1Q6; -.
DR PRIDE; Q9R1Q6; -.
DR ProteomicsDB; 263223; -.
DR Antibodypedia; 32900; 101 antibodies from 18 providers.
DR DNASU; 65963; -.
DR Ensembl; ENSMUST00000101429; ENSMUSP00000098972; ENSMUSG00000029810.
DR Ensembl; ENSMUST00000164733; ENSMUSP00000128705; ENSMUSG00000029810.
DR Ensembl; ENSMUST00000166247; ENSMUSP00000131064; ENSMUSG00000029810.
DR Ensembl; ENSMUST00000203355; ENSMUSP00000145395; ENSMUSG00000029810.
DR Ensembl; ENSMUST00000204073; ENSMUSP00000144864; ENSMUSG00000029810.
DR GeneID; 65963; -.
DR KEGG; mmu:65963; -.
DR UCSC; uc009bvu.3; mouse.
DR CTD; 28959; -.
DR MGI; MGI:1916348; Tmem176b.
DR VEuPathDB; HostDB:ENSMUSG00000029810; -.
DR eggNOG; ENOG502SF8T; Eukaryota.
DR GeneTree; ENSGT00530000064074; -.
DR HOGENOM; CLU_090530_0_0_1; -.
DR InParanoid; Q9R1Q6; -.
DR OMA; WEEDRCR; -.
DR OrthoDB; 1300114at2759; -.
DR PhylomeDB; Q9R1Q6; -.
DR TreeFam; TF335389; -.
DR BioGRID-ORCS; 65963; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Tmem176b; mouse.
DR PRO; PR:Q9R1Q6; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q9R1Q6; protein.
DR Bgee; ENSMUSG00000029810; Expressed in right kidney and 273 other tissues.
DR ExpressionAtlas; Q9R1Q6; baseline and differential.
DR Genevisible; Q9R1Q6; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0097028; P:dendritic cell differentiation; IMP:MGI.
DR GO; GO:2001199; P:negative regulation of dendritic cell differentiation; IMP:MGI.
DR InterPro; IPR007237; CD20-like.
DR InterPro; IPR009281; TMEM176A/TMEM176B.
DR PANTHER; PTHR15756; PTHR15756; 1.
DR Pfam; PF04103; CD20; 1.
PE 1: Evidence at protein level;
KW Differentiation; Membrane; Nucleus; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..263
FT /note="Transmembrane protein 176B"
FT /id="PRO_0000279876"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 239..263
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 231
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q3YBM2"
FT MOD_RES 240
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q3YBM2"
FT MOD_RES 253
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319"
FT CONFLICT 54
FT /note="I -> V (in Ref. 1; AAD28721)"
FT /evidence="ECO:0000305"
FT CONFLICT 260
FT /note="P -> S (in Ref. 1; AAD28721)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 263 AA; 28367 MW; 4D2C11EA67EB357C CRC64;
MVQSTVTVNG VKVASTHPQS AHISIHIHQK SALEQLLGAV GSLKKFLSWP QARIHYGQLS
LGVTQILLGL VSCALGVCLY FGPWTELCAF GCAFWSGSVA ILAGVGTIVH EKRQGKLSGQ
VSCLLLLACI ATAAAATVLG VNSLIRQTSV PYYVEIFSTC NPLQSSMDPG YGTVRYSDDS
DWKTERCREY LNMMMNLFLA FCIMLTVVCI LEIVVSVASL GLSLRSMYGR SSQALNEEES
ERKLLDGHPA PASPAKEKIP AIL
