ID   T1R1_MYCPN              Reviewed;         376 AA.
AC   P75431;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Putative type I restriction enzyme MpnIIP endonuclease subunit N-terminal part {ECO:0000303|PubMed:12654995, ECO:0000303|PubMed:8948633};
DE            Short=R protein N-terminal part;
DE   AltName: Full=Putative type I restriction enzyme MpnORFDP endonuclease subunit part 1;
DE            Short=MpnORFDAP;
GN   OrderedLocusNames=MPN_347; ORFNames=H91_orf376, MP489;
OS   Mycoplasma pneumoniae (strain ATCC 29342 / M129) (Mycoplasmoides
OS   pneumoniae).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=272634;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29342 / M129;
RX   PubMed=8948633; DOI=10.1093/nar/24.22.4420;
RA   Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.;
RT   "Complete sequence analysis of the genome of the bacterium Mycoplasma
RT   pneumoniae.";
RL   Nucleic Acids Res. 24:4420-4449(1996).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
RN   [3]
RP   DISCUSSION OF SEQUENCE.
RC   STRAIN=ATCC 29342 / M129;
RX   PubMed=23300489; DOI=10.1371/journal.pgen.1003191;
RA   Lluch-Senar M., Luong K., Llorens-Rico V., Delgado J., Fang G., Spittle K.,
RA   Clark T.A., Schadt E., Turner S.W., Korlach J., Serrano L.;
RT   "Comprehensive methylome characterization of Mycoplasma genitalium and
RT   Mycoplasma pneumoniae at single-base resolution.";
RL   PLoS Genet. 9:e1003191-e1003191(2013).
CC   -!- FUNCTION: The N-terminal section of a putative type I restriction
CC       enzyme that if reconstituted might recognize 5'-GAN(7)TAY-3' and cleave
CC       a random distance away (Probable). Subunit R is required for both
CC       nuclease and ATPase activities, but not for modification
CC       (PubMed:12654995). {ECO:0000303|PubMed:12654995,
CC       ECO:0000305|PubMed:8948633}.
CC   -!- CAUTION: Could be the product of a pseudogene; in this organism the
CC       endonuclease subunit carries 2 frameshift mutations (resulting in 3
CC       short ORFs) and is probably not expressed.
CC       {ECO:0000305|PubMed:23300489, ECO:0000305|PubMed:8948633}.
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DR   EMBL; U00089; AAB96137.1; -; Genomic_DNA.
DR   PIR; S73815; S73815.
DR   RefSeq; WP_010874703.1; NC_000912.1.
DR   AlphaFoldDB; P75431; -.
DR   SMR; P75431; -.
DR   IntAct; P75431; 1.
DR   STRING; 272634.MPN_347; -.
DR   EnsemblBacteria; AAB96137; AAB96137; MPN_347.
DR   KEGG; mpn:MPN_347; -.
DR   HOGENOM; CLU_735331_0_0_14; -.
DR   OMA; IQVNEDI; -.
DR   PRO; PR:P75431; -.
DR   Proteomes; UP000000808; Chromosome.
DR   GO; GO:0009035; F:type I site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR022625; TypeI_RM_Rsu_C.
DR   Pfam; PF12008; EcoR124_C; 1.
PE   5: Uncertain;
KW   Reference proteome; Restriction system.
FT   CHAIN           1..376
FT                   /note="Putative type I restriction enzyme MpnIIP
FT                   endonuclease subunit N-terminal part"
FT                   /id="PRO_0000077263"
SQ   SEQUENCE   376 AA;  45087 MW;  691BB674F64988D9 CRC64;
     MFLTGFDAPT LNTLWVDKNL SEHTLIQAFS RTNRCFGETK KFGNIFSFRD IRENFDQALK
     MYADKRGLKD IKSNMVMREF EEVFEKFKDW TEKIKQKYPN PDEILNLEKE EKWDFSDLFG
     EFLGDYRTVK SYHEFDKDNP YLPSDEFNKY FAAFSQIQEE IRKEKAEKKA LEVQEEDLSN
     EVRKEPQIFS WEYGEGIQAN LDYIFHLIKD LYNCDNEQDR QKVLDKIKTA IRTNPEIQVN
     EDILNEFIED LGKSFEKEKL EEVWKTEGKL RECLKDFCKK KEESNKKKII NDFLVKGVFL
     VEWVADKQLN TAKKKGEYSP SWSDTEKLIW KEKSTNLPTM TEKDEDGNFK WRKLITDISE
     AFKNHFERFI RFVEIR