ID   BP48_BPT4               Reviewed;         364 AA.
AC   P13339;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   29-SEP-2021, entry version 84.
DE   RecName: Full=Baseplate tail-tube junction protein gp48 {ECO:0000305};
DE   AltName: Full=Baseplate-tube cap {ECO:0000305};
DE   AltName: Full=Gene product 48;
DE            Short=gp48;
GN   Name=48;
OS   Enterobacteria phage T4 (Bacteriophage T4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10665;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13, AND FUNCTION.
RX   PubMed=3363870; DOI=10.1016/0042-6822(88)90622-8;
RA   Ishimoto L.K., Ishimoto K.S., Cascino A., Cipollaro M., Eiserling F.A.;
RT   "The structure of three bacteriophage T4 genes required for tail-tube
RT   assembly.";
RL   Virology 164:81-90(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA   Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT   "Bacteriophage T4 genome.";
RL   Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN   [3]
RP   SUBCELLULAR LOCATION, AND SUBUNIT.
RX   PubMed=2403438; DOI=10.1128/jvi.64.1.143-154.1990;
RA   Watts N.R., Coombs D.H.;
RT   "Structure of the bacteriophage T4 baseplate as determined by chemical
RT   cross-linking.";
RL   J. Virol. 64:143-154(1990).
RN   [4]
RP   REVIEW.
RX   PubMed=14625682; DOI=10.1007/s00018-003-3072-1;
RA   Leiman P.G., Kanamaru S., Mesyanzhinov V.V., Arisaka F., Rossmann M.G.;
RT   "Structure and morphogenesis of bacteriophage T4.";
RL   Cell. Mol. Life Sci. 60:2356-2370(2003).
RN   [5]
RP   REVIEW ON FUNCTION.
RX   PubMed=21129200; DOI=10.1186/1743-422x-7-355;
RA   Leiman P.G., Arisaka F., van Raaij M.J., Kostyuchenko V.A., Aksyuk A.A.,
RA   Kanamaru S., Rossmann M.G.;
RT   "Morphogenesis of the T4 tail and tail fibers.";
RL   Virol. J. 7:355-355(2010).
RN   [6]
RP   SUBUNIT.
RX   PubMed=19896486; DOI=10.1016/j.jmb.2009.10.071;
RA   Yap M.L., Mio K., Leiman P.G., Kanamaru S., Arisaka F.;
RT   "The baseplate wedges of bacteriophage T4 spontaneously assemble into
RT   hubless baseplate-like structure in vitro.";
RL   J. Mol. Biol. 395:349-360(2010).
RN   [7]
RP   STRUCTURE BY ELECTRON MICROSCOPY (4.11 ANGSTROMS), SUBUNIT, SUBCELLULAR
RP   LOCATION, AND FUNCTION.
RX   PubMed=27193680; DOI=10.1038/nature17971;
RA   Taylor N.M., Prokhorov N.S., Guerrero-Ferreira R.C., Shneider M.M.,
RA   Browning C., Goldie K.N., Stahlberg H., Leiman P.G.;
RT   "Structure of the T4 baseplate and its function in triggering sheath
RT   contraction.";
RL   Nature 533:346-352(2016).
CC   -!- FUNCTION: Baseplate protein that forms, together with gp54, the
CC       baseplate-tail tube junction. The tail tube first 2 annuli are formed
CC       by gp48 and gp54, which are in continuation of the spike complex.
CC       Involved in the tail assembly. Morphogenesis of the baseplate is
CC       completed by association of gp48 and gp54, which bind the upper part of
CC       the baseplate dome to form the platform for polymerization of the tail
CC       tube. {ECO:0000269|PubMed:27193680, ECO:0000269|PubMed:3363870,
CC       ECO:0000303|PubMed:21129200}.
CC   -!- SUBUNIT: Homohexamer (PubMed:27193680). The tube first annulus is
CC       composed of a gp48 hexameric ring. Interacts with gp54. Part of the
CC       baseplate macromolecular complex which consists of gp5, gp5.4, gp27
CC       (central spike complex); gp6, gp25, gp53 (inner baseplate); gp7, gp8
CC       (intermediate baseplate); gp9, gp10, gp11, gp12 (peripheral); gp48 and
CC       gp54 (proximal region of the tail tube). {ECO:0000269|PubMed:19896486,
CC       ECO:0000269|PubMed:2403438, ECO:0000269|PubMed:27193680}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:2403438,
CC       ECO:0000269|PubMed:27193680}. Note=Present in 6 copies in the
CC       baseplate. {ECO:0000303|PubMed:21129200}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000305}.
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DR   EMBL; M20298; AAA32539.1; -; Genomic_DNA.
DR   EMBL; AF158101; AAD42476.1; -; Genomic_DNA.
DR   PIR; JF0036; GCBPT4.
DR   RefSeq; NP_049806.1; NC_000866.4.
DR   PDB; 5IV5; EM; 4.11 A; BE/DH/GA/ID/U/r=1-364.
DR   PDBsum; 5IV5; -.
DR   TCDB; 1.K.1.1.1; the gp27/5 t4-baseplate (t4-bp) family.
DR   GeneID; 1258551; -.
DR   KEGG; vg:1258551; -.
DR   Proteomes; UP000009087; Genome.
DR   GO; GO:0098025; C:virus tail, baseplate; IDA:UniProtKB.
DR   GO; GO:0098003; P:viral tail assembly; IEA:UniProtKB-KW.
DR   InterPro; IPR024389; Gp48_T4-like.
DR   Pfam; PF11091; T4_tail_cap; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Late protein; Reference proteome;
KW   Viral baseplate protein; Viral release from host cell; Viral tail assembly;
KW   Viral tail protein; Virion.
FT   INIT_MET        1
FT                   /note="Removed; by host"
FT                   /evidence="ECO:0000269|PubMed:3363870"
FT   CHAIN           2..364
FT                   /note="Baseplate tail-tube junction protein gp48"
FT                   /id="PRO_0000165039"
SQ   SEQUENCE   364 AA;  39734 MW;  DB0E27909BB7C726 CRC64;
     MAIVKEITAD LIKKSGEKIS AGQSTKSEVG TKTYTAQFPT GRASGNDTTE DFQVTDLYKN
     GLLFTAYNMS SRDSGSLRSM RSNYSSSSSS ILRTARNTIS STVSKLSNGL ISNNNSGTIS
     KSPIANILLP RSKSDVDTSS HRFNDVQESL ISRGGGTATG VLSNIASTAV FGALESITQG
     IMADNNEQIY TTARSMYGGA ENRTKVFTWD LTPRSTEDLM AIINIYQYFN YFSYGETGKS
     QYAAEIKGYL DDWYRSTLIE PLSPEDAAKN KTLFEKMTSS LTNVLVVSNP TVWMVKNFGA
     TSKFDGKTEI FGPCQIQSIR FDKTPNGNFN GLAIAPNLPS TFTLEITMRE IITLNRASLY
     AGTF