ID   T1SC_MYCPN              Reviewed;         268 AA.
AC   P75416;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Putative type I specificity subunit S.MpnORF365P {ECO:0000303|PubMed:12654995};
DE            Short=S protein;
DE            Short=S.MpnORF365P {ECO:0000303|PubMed:12654995};
DE   AltName: Full=Putative type-1 restriction enzyme specificity subunit MPN_365;
DE   AltName: Full=S.MpnORFCP;
GN   OrderedLocusNames=MPN_365; ORFNames=H91_orf268, MP471;
OS   Mycoplasma pneumoniae (strain ATCC 29342 / M129) (Mycoplasmoides
OS   pneumoniae).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=272634;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29342 / M129;
RX   PubMed=8948633; DOI=10.1093/nar/24.22.4420;
RA   Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.;
RT   "Complete sequence analysis of the genome of the bacterium Mycoplasma
RT   pneumoniae.";
RL   Nucleic Acids Res. 24:4420-4449(1996).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
RN   [3]
RP   INDUCTION, AND DNA-BINDING.
RC   STRAIN=ATCC 29342 / M129;
RX   PubMed=23300489; DOI=10.1371/journal.pgen.1003191;
RA   Lluch-Senar M., Luong K., Llorens-Rico V., Delgado J., Fang G., Spittle K.,
RA   Clark T.A., Schadt E., Turner S.W., Korlach J., Serrano L.;
RT   "Comprehensive methylome characterization of Mycoplasma genitalium and
RT   Mycoplasma pneumoniae at single-base resolution.";
RL   PLoS Genet. 9:e1003191-e1003191(2013).
CC   -!- FUNCTION: The specificity (S) subunit of a type I methyltransferase
CC       (MTase); this subunit dictates DNA sequence specificity. The single R
CC       subunit has multiple frameshifts and is probably not expressed.
CC       {ECO:0000303|PubMed:12654995, ECO:0000305|PubMed:8948633}.
CC   -!- SUBUNIT: The methyltransferase is composed of M and S polypeptides.
CC       {ECO:0000250|UniProtKB:P05719}.
CC   -!- INDUCTION: Detected at low levels after 6 but not 96 hours growth (at
CC       protein level). {ECO:0000269|PubMed:23300489}.
CC   -!- DOMAIN: Contains two DNA recognition domains, each specifying
CC       recognition of one of the two defined components of the target
CC       sequence. {ECO:0000250|UniProtKB:P05719}.
CC   -!- SIMILARITY: Belongs to the type-I restriction system S methylase
CC       family. {ECO:0000305}.
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DR   EMBL; U00089; AAB96119.1; -; Genomic_DNA.
DR   PIR; S73797; S73797.
DR   AlphaFoldDB; P75416; -.
DR   SMR; P75416; -.
DR   IntAct; P75416; 4.
DR   REBASE; 6706; S.MpnORF365P.
DR   EnsemblBacteria; AAB96119; AAB96119; MPN_365.
DR   KEGG; mpn:MPN_365; -.
DR   HOGENOM; CLU_021095_6_2_14; -.
DR   OMA; NNACVIP; -.
DR   PRO; PR:P75416; -.
DR   Proteomes; UP000000808; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.220.20; -; 2.
DR   InterPro; IPR000055; Restrct_endonuc_typeI_TRD.
DR   InterPro; IPR044946; Restrct_endonuc_typeI_TRD_sf.
DR   Pfam; PF01420; Methylase_S; 2.
PE   1: Evidence at protein level;
KW   DNA-binding; Reference proteome; Restriction system.
FT   CHAIN           1..268
FT                   /note="Putative type I specificity subunit S.MpnORF365P"
FT                   /id="PRO_0000198046"
SQ   SEQUENCE   268 AA;  31147 MW;  2B7E2D3F826824DA CRC64;
     MEAPKHVNNA CVIPNLTLKK MREIELDFPS KKIQEKIATI LDTFTELSAE LRERKKQYAF
     YRDYLLNQEN IRKIYGANIP FETFQVKDIC EIRRGRAITK AYIRNNPGEN PVYSAATTND
     GELGRIKDCD FDGEYITWTT NGYAGVVFYR NGKFNASQDC GVLKVKNKKI CTKFLSFLLK
     IEAPKFVHNL ASRPKLSQKV MAEIELSFPP LEIQEKIADI LFAFEKLCND LVEGIPAEVE
     MRKKQLDYYQ NFLFNWVQEQ KTQLEQIM