ID   T1SH_MYCPN              Reviewed;         249 AA.
AC   P75180;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Putative type I specificity subunit S.MpnORF615P {ECO:0000303|PubMed:12654995};
DE            Short=S protein;
DE            Short=S.MpnORF615P {ECO:0000303|PubMed:12654995};
DE   AltName: Full=Putative type-1 specificity subunit MPN_615;
DE   AltName: Full=S.MpnORFHP;
GN   OrderedLocusNames=MPN_615; ORFNames=C12_orf249, MP227;
OS   Mycoplasma pneumoniae (strain ATCC 29342 / M129) (Mycoplasmoides
OS   pneumoniae).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=272634;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29342 / M129;
RX   PubMed=8948633; DOI=10.1093/nar/24.22.4420;
RA   Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.;
RT   "Complete sequence analysis of the genome of the bacterium Mycoplasma
RT   pneumoniae.";
RL   Nucleic Acids Res. 24:4420-4449(1996).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
RN   [3]
RP   INDUCTION, AND DNA-BINDING.
RC   STRAIN=ATCC 29342 / M129;
RX   PubMed=23300489; DOI=10.1371/journal.pgen.1003191;
RA   Lluch-Senar M., Luong K., Llorens-Rico V., Delgado J., Fang G., Spittle K.,
RA   Clark T.A., Schadt E., Turner S.W., Korlach J., Serrano L.;
RT   "Comprehensive methylome characterization of Mycoplasma genitalium and
RT   Mycoplasma pneumoniae at single-base resolution.";
RL   PLoS Genet. 9:e1003191-e1003191(2013).
CC   -!- FUNCTION: The specificity (S) subunit of a type I methyltransferase
CC       (MTase); this subunit dictates DNA sequence specificity. The single R
CC       subunit has multiple frameshifts and is probably not expressed.
CC       {ECO:0000303|PubMed:12654995, ECO:0000305|PubMed:8948633}.
CC   -!- SUBUNIT: The methyltransferase is composed of M and S polypeptides.
CC       {ECO:0000250|UniProtKB:P05719}.
CC   -!- INDUCTION: Detected at low levels after 6 and 96 hours growth, there
CC       are fewer copies at 96 hours (at protein level).
CC       {ECO:0000269|PubMed:23300489}.
CC   -!- DOMAIN: Contains two DNA recognition domains, each specifying
CC       recognition of one of the two defined components of the target
CC       sequence. {ECO:0000250|UniProtKB:P05719}.
CC   -!- SIMILARITY: Belongs to the type-I restriction system S methylase
CC       family. {ECO:0000305}.
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DR   EMBL; U00089; AAB95875.1; -; Genomic_DNA.
DR   PIR; S73553; S73553.
DR   AlphaFoldDB; P75180; -.
DR   SMR; P75180; -.
DR   IntAct; P75180; 2.
DR   REBASE; 6707; S.MpnORF615P.
DR   EnsemblBacteria; AAB95875; AAB95875; MPN_615.
DR   KEGG; mpn:MPN_615; -.
DR   HOGENOM; CLU_021095_6_2_14; -.
DR   PRO; PR:P75180; -.
DR   Proteomes; UP000000808; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.220.20; -; 1.
DR   InterPro; IPR000055; Restrct_endonuc_typeI_TRD.
DR   InterPro; IPR044946; Restrct_endonuc_typeI_TRD_sf.
DR   Pfam; PF01420; Methylase_S; 2.
PE   1: Evidence at protein level;
KW   DNA-binding; Reference proteome; Restriction system.
FT   CHAIN           1..249
FT                   /note="Putative type I specificity subunit S.MpnORF615P"
FT                   /id="PRO_0000198052"
SQ   SEQUENCE   249 AA;  28758 MW;  14AC1D1350DB5855 CRC64;
     MQGILAEIEL DFPPLQIQEK IATILDTFTE LSAELRERKK QYAFYRDYLL NQENIRKIYG
     ANIPFETFQV KDICEIRRGR AITKAYIRNN PGENPVYSAA TTNDGELGRI KDCDFDGEYI
     TWTTNGYAGV VFYRNGKFNA SQDCGVLKVK NKKICTKFLS FLLKIEAPKF VHNLASRPKL
     SQKVMAEIEL SFPPLEIQEK IADILFAFEK LCNDLVEGIP AEIEMRKKQL DYYYHLIFSK
     IAHFSKQLA