T1SX_MYCPN
ID T1SX_MYCPN Reviewed; 375 AA.
AC P75159;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Putative type I specificity subunit S.MpnORF638P {ECO:0000303|PubMed:12654995};
DE Short=S protein;
DE Short=S.MpnORF638P {ECO:0000303|PubMed:12654995};
GN OrderedLocusNames=MPN_638; ORFNames=E30_orf375, MP204;
OS Mycoplasma pneumoniae (strain ATCC 29342 / M129) (Mycoplasmoides
OS pneumoniae).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX NCBI_TaxID=272634;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29342 / M129;
RX PubMed=8948633; DOI=10.1093/nar/24.22.4420;
RA Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.;
RT "Complete sequence analysis of the genome of the bacterium Mycoplasma
RT pneumoniae.";
RL Nucleic Acids Res. 24:4420-4449(1996).
RN [2]
RP NOMENCLATURE.
RX PubMed=12654995; DOI=10.1093/nar/gkg274;
RA Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT endonucleases and their genes.";
RL Nucleic Acids Res. 31:1805-1812(2003).
RN [3]
RP INDUCTION, AND DNA-BINDING.
RC STRAIN=ATCC 29342 / M129;
RX PubMed=23300489; DOI=10.1371/journal.pgen.1003191;
RA Lluch-Senar M., Luong K., Llorens-Rico V., Delgado J., Fang G., Spittle K.,
RA Clark T.A., Schadt E., Turner S.W., Korlach J., Serrano L.;
RT "Comprehensive methylome characterization of Mycoplasma genitalium and
RT Mycoplasma pneumoniae at single-base resolution.";
RL PLoS Genet. 9:e1003191-e1003191(2013).
CC -!- FUNCTION: The specificity (S) subunit of a type I methyltransferase
CC (MTase); this subunit dictates DNA sequence specificity. The single R
CC subunit has multiple frameshifts and is probably not expressed.
CC {ECO:0000303|PubMed:12654995, ECO:0000305|PubMed:8948633}.
CC -!- SUBUNIT: The methyltransferase is composed of M and S polypeptides.
CC {ECO:0000250|UniProtKB:P05719}.
CC -!- INDUCTION: Highly expressed after 6 and 96 hours growth, there are
CC fewer copies at 96 hours (at protein level).
CC {ECO:0000269|PubMed:23300489}.
CC -!- DOMAIN: Contains two DNA recognition domains, each specifying
CC recognition of one of the two defined components of the target
CC sequence. {ECO:0000250|UniProtKB:P05719}.
CC -!- SIMILARITY: Belongs to the type-I restriction system S methylase
CC family. {ECO:0000305}.
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DR EMBL; U00089; AAB95852.1; -; Genomic_DNA.
DR PIR; S73530; S73530.
DR RefSeq; NP_110327.1; NC_000912.1.
DR RefSeq; WP_010874995.1; NC_000912.1.
DR AlphaFoldDB; P75159; -.
DR SMR; P75159; -.
DR IntAct; P75159; 1.
DR REBASE; 6863; S.MpnORF638P.
DR EnsemblBacteria; AAB95852; AAB95852; MPN_638.
DR KEGG; mpn:MPN_638; -.
DR PATRIC; fig|272634.6.peg.701; -.
DR HOGENOM; CLU_721242_0_0_14; -.
DR OMA; KFAYYAL; -.
DR BioCyc; MPNE272634:G1GJ3-1021-MON; -.
DR PRO; PR:P75159; -.
DR Proteomes; UP000000808; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 3.90.220.20; -; 2.
DR InterPro; IPR000055; Restrct_endonuc_typeI_TRD.
DR InterPro; IPR044946; Restrct_endonuc_typeI_TRD_sf.
DR Pfam; PF01420; Methylase_S; 2.
PE 1: Evidence at protein level;
KW DNA-binding; Reference proteome; Restriction system.
FT CHAIN 1..375
FT /note="Putative type I specificity subunit S.MpnORF638P"
FT /id="PRO_0000198043"
SQ SEQUENCE 375 AA; 42650 MW; 4DBAE8BED64A45FA CRC64;
MTPKLKLNTN SNWTKKTLGS LFELKKGEML EKELLAPDGK YEYFNGGIKA SGRTNEFNTF
KNTISIIIGG SCGYVRLADK DYFCGQSSCT LTVLDPLEID LKFAYYALKS QEEKITSLAS
GTTIKNIRLS DLKDLPIPLV KSIQDQRTIA HALSVFDLRI EHLNELIEVN RKLRDEYAHK
LFTLDPDFLT HWNLHELHEQ MGEISLGEVF HLKSGKYLKA DERFEDGKFP YYGAGIESTS
FVNEPNTKGD TLSMIANGYS IGNIRYHTIP WFNGTGGIAM EALKPNKTYV PFFYCALKYM
QKDLKERFKR DESPFISLKL AGEIKVPFVK SFALQRKAGK IIYLLDKTLE ECKEEAKSLI
SIRDNLLGKL FPTLS
