T200_SALTY
ID T200_SALTY Reviewed; 152 AA.
AC P59696; Q57334;
DT 23-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 23-MAY-2003, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Transposase for insertion sequence element IS200;
GN Name=tnpA1; OrderedLocusNames=STM0946;
GN and
GN Name=tnpA2; OrderedLocusNames=STM1957;
GN and
GN Name=tnpA3; OrderedLocusNames=STM2471;
GN and
GN Name=tnpA4; OrderedLocusNames=STM3032;
GN and
GN Name=tnpA5; OrderedLocusNames=STM3479;
GN and
GN Name=tnpA6; OrderedLocusNames=STM4311;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2 / ATCC 23564;
RA Burnens A.P., Stanley J., Hunziker P., Brodard I., Nicolet J.;
RT "Salmonella typhimurium carries a conserved IS200 insertion between the
RT flagellar genes fliA and fliB.";
RL Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=SARA17;
RX PubMed=8253675; DOI=10.1128/jb.175.24.7863-7868.1993;
RA Bisercic M., Ochman H.;
RT "The ancestry of insertion sequences common to Escherichia coli and
RT Salmonella typhimurium.";
RL J. Bacteriol. 175:7863-7868(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2;
RX PubMed=10464203; DOI=10.1128/jb.181.17.5317-5329.1999;
RA Kofoid E.C., Rappleye C.A., Stojiljkovic I., Roth J.R.;
RT "The 17-gene ethanolamine (eut) operon of Salmonella typhimurium encodes
RT five homologues of carboxysome shell proteins.";
RL J. Bacteriol. 181:5317-5329(1999).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
CC -!- FUNCTION: Transposase responsible for transposition of the IS200
CC insertion sequence (IS) element. Transposition occurs in 2 main steps,
CC excision from the donor DNA 'top strand' into a single strand circle
CC and its subsequent reinsertion into the DNA target. This increases the
CC copy number of the IS. {ECO:0000250|UniProtKB:Q7DF83}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:Q7DF83};
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q7DF83}.
CC -!- MISCELLANEOUS: Belongs to the IS200/IS605 insertion sequence (IS)
CC element family. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the transposase 17 family. {ECO:0000305}.
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DR EMBL; Z54217; CAA90952.1; -; Genomic_DNA.
DR EMBL; L25848; AAC36995.1; -; Unassigned_DNA.
DR EMBL; AF025380; AAB81521.1; -; Genomic_DNA.
DR EMBL; U44749; AAA86759.1; -; Genomic_DNA.
DR EMBL; AF093749; AAC78110.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL19882.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL20869.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL21365.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL21908.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL22341.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL23135.1; -; Genomic_DNA.
DR RefSeq; NP_459923.1; NC_003197.2.
DR RefSeq; NP_460910.3; NC_003197.2.
DR RefSeq; NP_461406.1; NC_003197.2.
DR RefSeq; NP_461949.1; NC_003197.2.
DR RefSeq; NP_462382.1; NC_003197.2.
DR RefSeq; NP_463176.1; NC_003197.2.
DR RefSeq; WP_000502119.1; NC_003197.2.
DR AlphaFoldDB; P59696; -.
DR SMR; P59696; -.
DR PaxDb; P59696; -.
DR DNASU; 1255837; -.
DR EnsemblBacteria; AAL19882; AAL19882; STM0946.
DR EnsemblBacteria; AAL20869; AAL20869; STM1957.
DR EnsemblBacteria; AAL21365; AAL21365; STM2471.
DR EnsemblBacteria; AAL21908; AAL21908; STM3032.
DR EnsemblBacteria; AAL22341; AAL22341; STM3479.
DR EnsemblBacteria; AAL23135; AAL23135; STM4311.
DR GeneID; 1252465; -.
DR GeneID; 1253478; -.
DR GeneID; 1253993; -.
DR GeneID; 1254555; -.
DR GeneID; 1255002; -.
DR GeneID; 1255837; -.
DR GeneID; 66758093; -.
DR KEGG; stm:STM0946; -.
DR KEGG; stm:STM1957; -.
DR KEGG; stm:STM2471; -.
DR KEGG; stm:STM3032; -.
DR KEGG; stm:STM3479; -.
DR KEGG; stm:STM4311; -.
DR PATRIC; fig|99287.12.peg.2609; -.
DR HOGENOM; CLU_101320_0_0_6; -.
DR OMA; WNCKYHV; -.
DR PhylomeDB; P59696; -.
DR BioCyc; SENT99287:STM4311-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004803; F:transposase activity; IEA:InterPro.
DR GO; GO:0006313; P:transposition, DNA-mediated; IEA:InterPro.
DR Gene3D; 3.30.70.1290; -; 1.
DR InterPro; IPR002686; Transposase_17.
DR InterPro; IPR036515; Transposase_17_sf.
DR Pfam; PF01797; Y1_Tnp; 1.
DR SMART; SM01321; Y1_Tnp; 1.
DR SUPFAM; SSF143422; SSF143422; 1.
PE 3: Inferred from homology;
KW DNA recombination; DNA-binding; Endonuclease; Hydrolase; Magnesium;
KW Metal-binding; Nuclease; Reference proteome; Transposable element;
KW Transposition.
FT CHAIN 1..152
FT /note="Transposase for insertion sequence element IS200"
FT /id="PRO_0000075490"
FT ACT_SITE 125
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q7DF83"
FT BINDING 61
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:Q7DF83"
FT BINDING 63
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:Q7DF83"
FT BINDING 129
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:Q7DF83"
SQ SEQUENCE 152 AA; 17958 MW; 8389733629EFBC19 CRC64;
MGDEKSLAHT RWNCKYHIVF APKYRRQAFY GEKRRAVGSI LRKLCEWKNV RILEAECCAD
HIHMLLEIPP KMSVSSFMGY LKGKSSLMLY EQFGDLKFKY RNREFWCRGY YVDTVGKNTA
KIQDYIKHQL EEDKMGEQLS IPYPGSPFTG RK
