BP53_BPT4
ID BP53_BPT4 Reviewed; 196 AA.
AC P16011;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 02-DEC-2020, entry version 81.
DE RecName: Full=Baseplate wedge protein gp53 {ECO:0000305};
DE AltName: Full=Gene product 53 {ECO:0000305};
DE Short=gp53;
GN Name=53;
OS Enterobacteria phage T4 (Bacteriophage T4).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX NCBI_TaxID=10665;
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2488704;
RA Mosig G., Lin G.W., Franklin J., Fan W.H.;
RT "Functional relationships and structural determinants of two bacteriophage
RT T4 lysozymes: a soluble (gene e) and a baseplate-associated (gene 5)
RT protein.";
RL New Biol. 1:171-179(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2740234; DOI=10.1093/nar/17.11.4392;
RA Koch T., Lamm N., Rueger W.;
RT "Sequencing, cloning and overexpression of genes of bacteriophage T4
RT between map positions 74.325 and 77.184.";
RL Nucleic Acids Res. 17:4392-4392(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT "Bacteriophage T4 genome.";
RL Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN [4]
RP PROTEIN SEQUENCE OF 1-6, AND SUBCELLULAR LOCATION.
RX PubMed=15342608; DOI=10.1128/jb.186.18.6335-6339.2004;
RA Ye N., Nemoto N.;
RT "Processing of the tail lysozyme (gp5) of bacteriophage T4.";
RL J. Bacteriol. 186:6335-6339(2004).
RN [5]
RP SUBCELLULAR LOCATION, AND SUBUNIT.
RX PubMed=2403438; DOI=10.1128/jvi.64.1.143-154.1990;
RA Watts N.R., Coombs D.H.;
RT "Structure of the bacteriophage T4 baseplate as determined by chemical
RT cross-linking.";
RL J. Virol. 64:143-154(1990).
RN [6]
RP REVIEW.
RX PubMed=14625682; DOI=10.1007/s00018-003-3072-1;
RA Leiman P.G., Kanamaru S., Mesyanzhinov V.V., Arisaka F., Rossmann M.G.;
RT "Structure and morphogenesis of bacteriophage T4.";
RL Cell. Mol. Life Sci. 60:2356-2370(2003).
RN [7]
RP REVIEW ON FUNCTION.
RX PubMed=21129200; DOI=10.1186/1743-422x-7-355;
RA Leiman P.G., Arisaka F., van Raaij M.J., Kostyuchenko V.A., Aksyuk A.A.,
RA Kanamaru S., Rossmann M.G.;
RT "Morphogenesis of the T4 tail and tail fibers.";
RL Virol. J. 7:355-355(2010).
RN [8]
RP SUBUNIT.
RX PubMed=19896486; DOI=10.1016/j.jmb.2009.10.071;
RA Yap M.L., Mio K., Leiman P.G., Kanamaru S., Arisaka F.;
RT "The baseplate wedges of bacteriophage T4 spontaneously assemble into
RT hubless baseplate-like structure in vitro.";
RL J. Mol. Biol. 395:349-360(2010).
RN [9]
RP STRUCTURE BY ELECTRON MICROSCOPY (17.0 ANGSTROMS) OF THE CONTRACTED TAIL,
RP SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=15315755; DOI=10.1016/j.cell.2004.07.022;
RA Leiman P.G., Chipman P.R., Kostyuchenko V.A., Mesyanzhinov V.V.,
RA Rossmann M.G.;
RT "Three-dimensional rearrangement of proteins in the tail of bacteriophage
RT T4 on infection of its host.";
RL Cell 118:419-429(2004).
RN [10]
RP STRUCTURE BY ELECTRON MICROSCOPY (4.11 ANGSTROMS), SUBUNIT, SUBCELLULAR
RP LOCATION, AND FUNCTION.
RX PubMed=27193680; DOI=10.1038/nature17971;
RA Taylor N.M., Prokhorov N.S., Guerrero-Ferreira R.C., Shneider M.M.,
RA Browning C., Goldie K.N., Stahlberg H., Leiman P.G.;
RT "Structure of the T4 baseplate and its function in triggering sheath
RT contraction.";
RL Nature 533:346-352(2016).
CC -!- FUNCTION: Baseplate protein that is located next to the tail tube
CC (inner baseplate). Involved in the tail assembly. Involved in the tail
CC assembly. {ECO:0000269|PubMed:15315755, ECO:0000269|PubMed:27193680,
CC ECO:0000303|PubMed:21129200}.
CC -!- SUBUNIT: Part of the baseplate macromolecular complex which consists of
CC gp5, gp5.4, gp27 (central spike complex); gp6, gp25, gp53 (inner
CC baseplate); gp7, gp8 (intermediate baseplate); gp9, gp10, gp11, gp12
CC (peripheral); gp48 and gp54 (proximal region of the tail tube).
CC Interacts with gp25 and with the (gp6)2-gp7 heterotrimeric molecule.
CC {ECO:0000269|PubMed:19896486, ECO:0000269|PubMed:2403438,
CC ECO:0000269|PubMed:27193680}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:15315755,
CC ECO:0000269|PubMed:15342608, ECO:0000269|PubMed:2403438,
CC ECO:0000269|PubMed:27193680}. Note=Present in 6 copies in the
CC baseplate. {ECO:0000303|PubMed:21129200}.
CC -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC {ECO:0000305}.
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DR EMBL; X15728; CAA33748.1; -; Genomic_DNA.
DR EMBL; X14845; CAA32949.1; -; Genomic_DNA.
DR EMBL; AF158101; AAD42489.1; -; Genomic_DNA.
DR PIR; S25239; GPBP84.
DR RefSeq; NP_049756.1; NC_000866.4.
DR PDB; 5HX2; EM; 3.80 A; F=1-196.
DR PDB; 5IV5; EM; 4.11 A; BF/DI/GB/IE/V/s=1-196.
DR PDB; 5IV7; EM; 6.77 A; BE/DG/GB/P/f/v=1-196.
DR PDBsum; 5HX2; -.
DR PDBsum; 5IV5; -.
DR PDBsum; 5IV7; -.
DR SMR; P16011; -.
DR TCDB; 1.K.1.1.1; the gp27/5 t4-baseplate (t4-bp) family.
DR GeneID; 1258693; -.
DR KEGG; vg:1258693; -.
DR Proteomes; UP000009087; Genome.
DR GO; GO:0098025; C:virus tail, baseplate; IDA:UniProtKB.
DR GO; GO:0098003; P:viral tail assembly; IEA:UniProtKB-KW.
DR InterPro; IPR022607; Phage_T4_Gp53_baseplate_wedge.
DR Pfam; PF11246; Phage_gp53; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Late protein; Reference proteome;
KW Viral baseplate protein; Viral release from host cell; Viral tail assembly;
KW Viral tail protein; Virion.
FT CHAIN 1..196
FT /note="Baseplate wedge protein gp53"
FT /id="PRO_0000165042"
SQ SEQUENCE 196 AA; 22966 MW; F59E2E42FFE95B00 CRC64;
MLFTFFDPIE YAAKTVNKNA PTIPMTDIFR NYKDYFKRAL AGYRLRTYYI KGSPRPEELA
NAIYGNPQLY WVLLMCNDNY DPYYGWITSQ EAAYQASIQK YKNVGGDQIV YHVNENGEKF
YNLISYDDNP YVWYDKGDKA RKYPQYEGAL AAVDTYEAAV LENEKLRQIK IIAKSDINSF
MNDLIRIMEK SYGNDK
