ID   T232_BACTB              Reviewed;         431 AA.
AC   Q99335;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Transposase for insertion sequence element IS232;
OS   Bacillus thuringiensis subsp. berliner.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1434;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1715;
RX   PubMed=2174857; DOI=10.1128/jb.172.12.6689-6696.1990;
RA   Menou G., Mahillon J., Lecadet M.-M., Lereclus D.;
RT   "Structural and genetic organization of IS232, a new insertion sequence of
RT   Bacillus thuringiensis.";
RL   J. Bacteriol. 172:6689-6696(1990).
CC   -!- FUNCTION: Involved in the transposition of the insertion sequence.
CC   -!- SIMILARITY: Belongs to the transposase IS21/IS408/IS1162 family.
CC       {ECO:0000305}.
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DR   EMBL; M38370; AAA98140.1; -; Genomic_DNA.
DR   PIR; A37801; A37801.
DR   AlphaFoldDB; Q99335; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0032196; P:transposition; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR017894; HTH_IS21_transposase_type.
DR   InterPro; IPR001584; Integrase_cat-core.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF00665; rve; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS50531; HTH_IS21; 1.
DR   PROSITE; PS50994; INTEGRASE; 1.
PE   3: Inferred from homology;
KW   DNA recombination; DNA-binding; Transposable element; Transposition.
FT   CHAIN           1..431
FT                   /note="Transposase for insertion sequence element IS232"
FT                   /id="PRO_0000075465"
FT   DOMAIN          20..79
FT                   /note="HTH IS21-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00615"
FT   DOMAIN          140..315
FT                   /note="Integrase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00457"
FT   DNA_BIND        35..54
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00615"
SQ   SEQUENCE   431 AA;  49894 MW;  3FB218504A50767A CRC64;
     MYIKLDIQTE FEVKSLSDLP NFKKLMGNLK MKINKSQLAR ELNVDRRTID KYLNGFTPKG
     TKNKTSKIDT YYEVIAALLS SDSKQIFYYK RVLWQYLTDN HGLKCSQSAF RAYINRKPEF
     RTYFDEGKRI LSGHSVGVRY ETPPGEQAQL DWKESIRFET KSGEIVYVNV AVLLLSYSRF
     KVFHLNISKS QSVLMSFMTE AFEMFGGVPK VIVTDNMKTV MDEARTEHFT GTINNKFAQF
     AQDFGFKVQP CIAGRPNTKG KVEAPMKLLD EIHTYQGRFT FEELHEFVQK LCARINQTFH
     QGTGKIPVFA LKQEKNLLQP LPKSAIRDSY MIKHKLVKVN TSGMISYKSN QYSVPAEYQG
     KTVGLQVYDN QIYVYHNMKL IVQHKISQSK LNYKEEHYKK ALAKSLPKYP NIDNLAKQNL
     SVIGEVYRNE E