ID   BPA_MYCS2               Reviewed;         175 AA.
AC   A0R5Z0;
DT   14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Bacterial proteasome activator {ECO:0000303|PubMed:25469515};
GN   Name=bpa {ECO:0000303|PubMed:25469515};
GN   OrderedLocusNames=MSMEG_6365 {ECO:0000312|EMBL:ABK74607.1},
GN   MSMEI_6197 {ECO:0000312|EMBL:AFP42623.1};
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   FUNCTION, INTERACTION WITH THE PROTEASOME SUBUNIT ALPHA PRCA, AND
RP   MUTAGENESIS OF 172-GLY--LEU-175.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=25469515; DOI=10.1371/journal.pone.0114348;
RA   Delley C.L., Laederach J., Ziemski M., Bolten M., Boehringer D.,
RA   Weber-Ban E.;
RT   "Bacterial proteasome activator Bpa (Rv3780) is a novel ring-shaped
RT   interactor of the mycobacterial proteasome.";
RL   PLoS ONE 9:E114348-E114348(2014).
CC   -!- FUNCTION: Interacts with the core proteasome alpha-subunit (PrcA)
CC       through its C-terminal hydrophobic-tyrosine-X motif (HbYX motif)
CC       (PubMed:25469515). Interaction of Bpa with the proteasome stimulates
CC       proteosomal peptidase and casein degradation activity, which suggests
CC       Bpa could play a role in the removal of non-native or damaged proteins
CC       by influencing the conformation of the proteasome complex upon
CC       interaction (By similarity). {ECO:0000250|UniProtKB:P9WKX3,
CC       ECO:0000269|PubMed:25469515}.
CC   -!- SUBUNIT: Forms a homooligomeric, either hexameric or heptameric, ring-
CC       like structure which stacks co-axially with the proteasomal alpha-
CC       rings. {ECO:0000250|UniProtKB:P9WKX3}.
CC   -!- SIMILARITY: Belongs to the Bpa family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000480; ABK74607.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP42623.1; -; Genomic_DNA.
DR   RefSeq; WP_011731234.1; NZ_SIJM01000013.1.
DR   RefSeq; YP_890578.1; NC_008596.1.
DR   AlphaFoldDB; A0R5Z0; -.
DR   SMR; A0R5Z0; -.
DR   STRING; 246196.MSMEI_6197; -.
DR   EnsemblBacteria; ABK74607; ABK74607; MSMEG_6365.
DR   EnsemblBacteria; AFP42623; AFP42623; MSMEI_6197.
DR   GeneID; 66737643; -.
DR   KEGG; msg:MSMEI_6197; -.
DR   KEGG; msm:MSMEG_6365; -.
DR   PATRIC; fig|246196.19.peg.6193; -.
DR   eggNOG; ENOG502ZPJ4; Bacteria.
DR   OMA; TDMVEQP; -.
DR   OrthoDB; 1767315at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0000502; C:proteasome complex; IEA:UniProtKB-KW.
DR   GO; GO:0070628; F:proteasome binding; IDA:UniProtKB.
DR   GO; GO:0061136; P:regulation of proteasomal protein catabolic process; IEA:InterPro.
DR   InterPro; IPR019695; Proteasome_act.
DR   Pfam; PF10759; DUF2587; 1.
PE   1: Evidence at protein level;
KW   Proteasome; Reference proteome.
FT   CHAIN           1..175
FT                   /note="Bacterial proteasome activator"
FT                   /id="PRO_0000434132"
FT   REGION          152..175
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           173..175
FT                   /note="HbYX motif"
FT                   /evidence="ECO:0000250|UniProtKB:P9WKX3"
FT   MUTAGEN         172..175
FT                   /note="Missing: Loss of interaction with the proteasome
FT                   subunit PrcA."
FT                   /evidence="ECO:0000269|PubMed:25469515"
SQ   SEQUENCE   175 AA;  18944 MW;  99C7963D6ED82B42 CRC64;
     MTINPDDDNI EILTGAAGGA DTEGEGEGEG KSLTDLVEQP AKVMRIGTMI KQLLEEVRAA
     PLDDASRNRL REIHQTSIRE LEDGLAPELR EELERLTLPF TDDNVPSDAE LRIAQAQLVG
     WLEGLFHGIQ TALFAQQMAA RAQLEQMRQG ALPPGIQVPG AQRGGATHPG TGQYL