T254A_MOUSE
ID T254A_MOUSE Reviewed; 123 AA.
AC P0DN89; F6V0T5; Q9D890; Q9DCV5;
DT 11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT 11-MAY-2016, sequence version 1.
DT 03-AUG-2022, entry version 48.
DE RecName: Full=Transmembrane protein 254;
GN Name=Tmem254a; Synonyms=D14Ertd449e, Tmem254;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Kidney, and Small intestine;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, and Liver;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P0DN89-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P0DN89-2; Sequence=VSP_058265;
CC -!- CAUTION: Three tandem Tmem254 paralogs have been identified on
CC chromosome 14, Tmem254a, Tmem254b and Tmem254c. In the coding region,
CC their nucleotide sequences are identical. {ECO:0000305}.
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DR EMBL; AK002441; BAB22103.1; -; mRNA.
DR EMBL; AK008299; BAB25584.1; -; mRNA.
DR EMBL; AC165285; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC031854; AAH31854.1; -; mRNA.
DR CCDS; CCDS26876.1; -. [P0DN89-2]
DR CCDS; CCDS49420.1; -. [P0DN89-1]
DR RefSeq; NP_001257424.1; NM_001270495.1. [P0DN89-1]
DR RefSeq; NP_001257425.1; NM_001270496.1. [P0DN89-2]
DR RefSeq; NP_001257427.1; NM_001270498.1. [P0DN89-1]
DR RefSeq; NP_001257428.1; NM_001270499.1. [P0DN89-2]
DR RefSeq; NP_079587.1; NM_025311.3. [P0DN89-2]
DR RefSeq; NP_080955.1; NM_026679.2. [P0DN89-1]
DR AlphaFoldDB; P0DN89; -.
DR IntAct; P0DN89; 1.
DR PhosphoSitePlus; P0DN89; -.
DR jPOST; P0DN89; -.
DR PRIDE; P0DN89; -.
DR Antibodypedia; 29924; 80 antibodies from 18 providers.
DR DNASU; 66039; -.
DR Ensembl; ENSMUST00000100811; ENSMUSP00000098374; ENSMUSG00000072676.
DR Ensembl; ENSMUST00000185006; ENSMUSP00000139270; ENSMUSG00000072676. [P0DN89-1]
DR GeneID; 66039; -.
DR KEGG; mmu:66039; -.
DR UCSC; uc007srx.2; mouse.
DR CTD; 80195; -.
DR MGI; MGI:1196450; Tmem254a.
DR VEuPathDB; HostDB:ENSMUSG00000021867; -.
DR VEuPathDB; HostDB:ENSMUSG00000072676; -.
DR VEuPathDB; HostDB:ENSMUSG00000072680; -.
DR OMA; LLYKGWW; -.
DR OrthoDB; 1399874at2759; -.
DR TreeFam; TF328617; -.
DR BioGRID-ORCS; 100039192; 1 hit in 30 CRISPR screens.
DR BioGRID-ORCS; 100039257; 0 hits in 31 CRISPR screens.
DR BioGRID-ORCS; 66039; 0 hits in 33 CRISPR screens.
DR PRO; PR:P0DN89; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; P0DN89; protein.
DR Bgee; ENSMUSG00000072676; Expressed in vasculature of organ and 91 other tissues.
DR ExpressionAtlas; P0DN89; baseline and differential.
DR Genevisible; Q9DCV5; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR028110; TMEM254.
DR PANTHER; PTHR34104; PTHR34104; 1.
DR Pfam; PF14934; DUF4499; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..123
FT /note="Transmembrane protein 254"
FT /id="PRO_0000089797"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 63..83
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..29
FT /note="MGTATGAGYFQRGSLFWFTVITVSFGYYT -> MVVAKSEARRDPTAYFRVA
FT RLWPSLITALGLGYFA (in isoform 2)"
FT /id="VSP_058265"
SQ SEQUENCE 123 AA; 14239 MW; ABC62DC390497C2C CRC64;
MGTATGAGYF QRGSLFWFTV ITVSFGYYTW AVFWPQSIPY QSLGPLGPFT KYLVDHYHTF
LRNGYWLAWL IHVGESLYAL VLCKRKGITD VQAQLLWFLQ TFLFGVASLS ILIAYRSKRQ
KHN
