ID   T254B_MOUSE             Reviewed;         123 AA.
AC   P0DN90; Q9DCV5;
DT   11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT   11-MAY-2016, sequence version 1.
DT   03-AUG-2022, entry version 45.
DE   RecName: Full=Transmembrane protein 254b;
GN   Name=Tmem254b;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Kidney, and Liver;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=P0DN90-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=P0DN90-2; Sequence=VSP_058267;
CC   -!- CAUTION: Three tandem Tmem254 paralogs have been identified on
CC       chromosome 14, Tmem254a, Tmem254b and Tmem254c. In the coding region,
CC       their nucleotide sequences are identical. {ECO:0000305}.
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DR   EMBL; AC174479; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   CCDS; CCDS26876.1; -. [P0DN90-2]
DR   CCDS; CCDS49420.1; -. [P0DN90-1]
DR   RefSeq; NP_001257424.1; NM_001270495.1. [P0DN90-1]
DR   RefSeq; NP_001257427.1; NM_001270498.1. [P0DN90-1]
DR   RefSeq; NP_080955.1; NM_026679.2. [P0DN90-1]
DR   AlphaFoldDB; P0DN90; -.
DR   jPOST; P0DN90; -.
DR   Antibodypedia; 29924; 80 antibodies from 18 providers.
DR   DNASU; 66039; -.
DR   Ensembl; ENSMUST00000100811; ENSMUSP00000098374; ENSMUSG00000072676. [P0DN90-2]
DR   Ensembl; ENSMUST00000185006; ENSMUSP00000139270; ENSMUSG00000072676.
DR   GeneID; 66039; -.
DR   KEGG; mmu:66039; -.
DR   CTD; 80195; -.
DR   MGI; MGI:3710397; Tmem254b.
DR   VEuPathDB; HostDB:ENSMUSG00000021867; -.
DR   VEuPathDB; HostDB:ENSMUSG00000072676; -.
DR   VEuPathDB; HostDB:ENSMUSG00000072680; -.
DR   OMA; LLYKGWW; -.
DR   BioGRID-ORCS; 100039192; 1 hit in 30 CRISPR screens.
DR   BioGRID-ORCS; 100039257; 0 hits in 31 CRISPR screens.
DR   BioGRID-ORCS; 66039; 0 hits in 33 CRISPR screens.
DR   ChiTaRS; Tmem254b; mouse.
DR   PRO; PR:P0DN90; -.
DR   Proteomes; UP000000589; Chromosome 14.
DR   RNAct; P0DN90; protein.
DR   Bgee; ENSMUSG00000072676; Expressed in vasculature of organ and 91 other tissues.
DR   ExpressionAtlas; P0DN90; baseline and differential.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR028110; TMEM254.
DR   PANTHER; PTHR34104; PTHR34104; 1.
DR   Pfam; PF14934; DUF4499; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..123
FT                   /note="Transmembrane protein 254b"
FT                   /id="PRO_0000436162"
FT   TRANSMEM        15..35
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        63..83
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        95..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         1..29
FT                   /note="MGTATGAGYFQRGSLFWFTVITVSFGYYT -> MVVAKSEARRDPTAYFRVA
FT                   RLWPSLITALGLGYFA (in isoform 2)"
FT                   /id="VSP_058267"
SQ   SEQUENCE   123 AA;  14239 MW;  ABC62DC390497C2C CRC64;
     MGTATGAGYF QRGSLFWFTV ITVSFGYYTW AVFWPQSIPY QSLGPLGPFT KYLVDHYHTF
     LRNGYWLAWL IHVGESLYAL VLCKRKGITD VQAQLLWFLQ TFLFGVASLS ILIAYRSKRQ
     KHN