ID   T2A1_ANAVA              Reviewed;         315 AA.
AC   P0A458; P70803;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=Type II restriction enzyme AvaI {ECO:0000303|PubMed:12654995};
DE            Short=R.AvaI;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease AvaI;
DE   AltName: Full=Type-2 restriction enzyme AvaI;
GN   Name=avaIR {ECO:0000303|PubMed:8917312};
OS   Anabaena variabilis.
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Trichormus.
OX   NCBI_TaxID=264691;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=PCC 7118 / ATCC 27892;
RX   PubMed=8917312; DOI=10.1007/bf02173975;
RA   Ruan H., Lunnen K.D., Scott M.E., Moran L.S., Slatko B.E., Pelletier J.J.,
RA   Hess E.J., Benner J. II, Wilson G.G., Xu S.-Y.;
RT   "Cloning and sequence comparison of AvaI and BsoBI restriction-modification
RT   systems.";
RL   Mol. Gen. Genet. 252:695-699(1996).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-CYCGRG-3' and cleaves after C-1.
CC       {ECO:0000303|PubMed:12654995, ECO:0000305|PubMed:8917312}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; X98339; CAA66985.1; -; Genomic_DNA.
DR   PIR; S72472; S72472.
DR   AlphaFoldDB; P0A458; -.
DR   SMR; P0A458; -.
DR   PRIDE; P0A458; -.
DR   PRO; PR:P0A458; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.238.90; -; 1.
DR   InterPro; IPR043091; Restr_endonucII_AvaI/BsoBI_hel.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR015277; Restrct_endonuc_II_AvaI/BsoBI.
DR   Pfam; PF09194; Endonuc-BsobI; 1.
DR   SUPFAM; SSF52980; SSF52980; 1.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Restriction system.
FT   CHAIN           1..315
FT                   /note="Type II restriction enzyme AvaI"
FT                   /id="PRO_0000077280"
SQ   SEQUENCE   315 AA;  35679 MW;  6F22C7CE4D329E33 CRC64;
     MPYQYHIQSN DDLVTPYQEV RAGFVALALE RNRKATPFVE QARALKIRVS QIERPQDLLQ
     MRDIRPTLLA ASGVSDKAAG HLQEQDKVDA IEGLIQNFLE PAGENFVEEL VYRFLLTRGD
     TLGGSMRNVG GILAERKFAR YIISALTLSN TSYKWLDKNS KTWLNQPDDD TDIELRLRGL
     SWNLEGRNRT FIYNVNVPIV RKNIDICLFD CRQNEIEKNI ISNPNIYIAL GELKGGIDPA
     GADEHWKTAN SALARIRTAF DRHSLKPYTF FVGSAIEKSM AEEIWHQLNS GILTNAANLT
     QPDQVASLCA WFIQL