T2A1_AZOBR
ID T2A1_AZOBR Reviewed; 222 AA.
AC P26919;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 25-MAY-2022, entry version 65.
DE RecName: Full=Type II restriction enzyme AbrI {ECO:0000303|PubMed:12654995};
DE Short=R.AbrI;
DE EC=3.1.21.4;
DE AltName: Full=Endonuclease AbrI;
DE AltName: Full=Type-2 restriction enzyme AbrI;
GN Name=abrIR;
OS Azospirillum brasilense.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC Azospirillaceae; Azospirillum.
OX NCBI_TaxID=192;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 29711 / DSM 1844 / Sp35;
RA Schwabe G., Helke A., Klingmueller W.;
RT "AbrI restriction modification system from Azospirillium brasilense,
RT molecular cloning and characterization of its genes.";
RL Submitted (OCT-1991) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NOMENCLATURE, AND SUBTYPE.
RX PubMed=12654995; DOI=10.1093/nar/gkg274;
RA Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT endonucleases and their genes.";
RL Nucleic Acids Res. 31:1805-1812(2003).
CC -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC stranded sequence 5'-CTCGAG-3' and cleaves after C-1.
CC {ECO:0000303|PubMed:12654995}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC -!- SIMILARITY: Belongs to the XhoI type II restriction endonuclease
CC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X62690; CAA44567.1; -; Genomic_DNA.
DR AlphaFoldDB; P26919; -.
DR REBASE; 191893; Apa1447ORF1853P.
DR REBASE; 353100; LxyHY24ORF1216P.
DR REBASE; 8; AbrI.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR InterPro; IPR007636; Restrct_endonuc_II_XhoI.
DR Pfam; PF04555; XhoI; 1.
DR PIRSF; PIRSF000994; Restrict_endonuc_II_XhoI; 1.
PE 3: Inferred from homology;
KW Endonuclease; Hydrolase; Nuclease; Restriction system.
FT CHAIN 1..222
FT /note="Type II restriction enzyme AbrI"
FT /id="PRO_0000077277"
FT REGION 21..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 161..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 174..203
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 204..222
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 222 AA; 25260 MW; 8FB124EFC4BBDCC1 CRC64;
MALELADYER KARESVMAFW GNREKARQKQ QESGKPDQGE RRRDRRKNMD GFCALVIDII
RANGLHRAEI HQKRALLTLP GYFRPTKLWD LLVIQDRRLV AALEFKSQVG PSFGNNFNNR
TEEAIGTAHD LWTAYREGAF GQSPRPFVGW LMLVEDAPES NQRRADTSPH FRCSRSSRAH
PTYGGMTSSA SGSSRSSFTP RPRSSPRRAP PPRQGRIPSP RI
