ID   T2A1_NOSS1              Reviewed;         315 AA.
AC   P0A457; P70803;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Type II restriction enzyme AvaI {ECO:0000303|PubMed:12654995};
DE            Short=R.AvaI;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease AvaI;
DE   AltName: Full=Type-2 restriction enzyme AvaI;
GN   Name=avaIR; OrderedLocusNames=all3631;
OS   Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=103690;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX   PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA   Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA   Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA   Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT   "Complete genomic sequence of the filamentous nitrogen-fixing
RT   cyanobacterium Anabaena sp. strain PCC 7120.";
RL   DNA Res. 8:205-213(2001).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-CYCGRG-3' and cleaves after C-1.
CC       {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; BA000019; BAB75330.1; -; Genomic_DNA.
DR   PIR; AH2259; AH2259.
DR   RefSeq; WP_010997776.1; NZ_RSCN01000070.1.
DR   AlphaFoldDB; P0A457; -.
DR   SMR; P0A457; -.
DR   STRING; 103690.17132764; -.
DR   REBASE; 165; AvaI.
DR   EnsemblBacteria; BAB75330; BAB75330; BAB75330.
DR   KEGG; ana:all3631; -.
DR   eggNOG; ENOG502Z94D; Bacteria.
DR   OMA; AGADEHW; -.
DR   OrthoDB; 615589at2; -.
DR   PRO; PR:P0A457; -.
DR   Proteomes; UP000002483; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.238.90; -; 1.
DR   InterPro; IPR043091; Restr_endonucII_AvaI/BsoBI_hel.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR015277; Restrct_endonuc_II_AvaI/BsoBI.
DR   Pfam; PF09194; Endonuc-BsobI; 1.
DR   SUPFAM; SSF52980; SSF52980; 1.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Reference proteome; Restriction system.
FT   CHAIN           1..315
FT                   /note="Type II restriction enzyme AvaI"
FT                   /id="PRO_0000077279"
SQ   SEQUENCE   315 AA;  35679 MW;  6F22C7CE4D329E33 CRC64;
     MPYQYHIQSN DDLVTPYQEV RAGFVALALE RNRKATPFVE QARALKIRVS QIERPQDLLQ
     MRDIRPTLLA ASGVSDKAAG HLQEQDKVDA IEGLIQNFLE PAGENFVEEL VYRFLLTRGD
     TLGGSMRNVG GILAERKFAR YIISALTLSN TSYKWLDKNS KTWLNQPDDD TDIELRLRGL
     SWNLEGRNRT FIYNVNVPIV RKNIDICLFD CRQNEIEKNI ISNPNIYIAL GELKGGIDPA
     GADEHWKTAN SALARIRTAF DRHSLKPYTF FVGSAIEKSM AEEIWHQLNS GILTNAANLT
     QPDQVASLCA WFIQL