T2AG_SCHPO
ID T2AG_SCHPO Reviewed; 109 AA.
AC O74948;
DT 23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2012, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Transcription initiation factor IIA subunit 2;
DE AltName: Full=General transcription factor IIA subunit 2;
DE AltName: Full=Transcription initiation factor IIA small chain;
GN Name=toa2; ORFNames=SPCC553.11c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP REVISION OF GENE MODEL.
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: TFIIA is a component of the transcription machinery of RNA
CC polymerase II and plays an important role in transcriptional
CC activation. TFIIA in a complex with tbp mediates transcriptional
CC activity (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: TFIIA is a heterodimer composed of the large toa1 and the
CC small toa2 subunits. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16823372}. Cytoplasm
CC {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the TFIIA subunit 2 family. {ECO:0000305}.
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DR EMBL; CU329672; CAA19263.2; -; Genomic_DNA.
DR PIR; T41393; T41393.
DR RefSeq; NP_587763.2; NM_001022756.2.
DR AlphaFoldDB; O74948; -.
DR SMR; O74948; -.
DR BioGRID; 275439; 3.
DR STRING; 4896.SPCC553.11c.1; -.
DR MaxQB; O74948; -.
DR PaxDb; O74948; -.
DR PRIDE; O74948; -.
DR EnsemblFungi; SPCC553.11c.1; SPCC553.11c.1:pep; SPCC553.11c.
DR GeneID; 2538859; -.
DR KEGG; spo:SPCC553.11c; -.
DR PomBase; SPCC553.11c; toa2.
DR VEuPathDB; FungiDB:SPCC553.11c; -.
DR eggNOG; KOG3463; Eukaryota.
DR HOGENOM; CLU_112964_3_1_1; -.
DR InParanoid; O74948; -.
DR OMA; QYYELYR; -.
DR Reactome; R-SPO-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-SPO-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-SPO-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-SPO-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-SPO-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-SPO-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-SPO-9018519; Estrogen-dependent gene expression.
DR PRO; PR:O74948; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0005672; C:transcription factor TFIIA complex; ISO:PomBase.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; ISO:PomBase.
DR GO; GO:0017025; F:TBP-class protein binding; IBA:GO_Central.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IBA:GO_Central.
DR CDD; cd10014; TFIIA_gamma_C; 1.
DR CDD; cd10145; TFIIA_gamma_N; 1.
DR Gene3D; 1.10.287.190; -; 1.
DR Gene3D; 2.30.18.10; -; 1.
DR InterPro; IPR009083; TFIIA_a-hlx.
DR InterPro; IPR009088; TFIIA_b-brl.
DR InterPro; IPR003194; TFIIA_gsu.
DR InterPro; IPR015871; TFIIA_gsu_C.
DR InterPro; IPR015872; TFIIA_gsu_N.
DR PANTHER; PTHR10966; PTHR10966; 1.
DR Pfam; PF02751; TFIIA_gamma_C; 1.
DR Pfam; PF02268; TFIIA_gamma_N; 1.
DR PIRSF; PIRSF009415; Hum_TFIIA_gamma; 1.
DR SUPFAM; SSF50784; SSF50784; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..109
FT /note="Transcription initiation factor IIA subunit 2"
FT /id="PRO_0000194052"
SQ SEQUENCE 109 AA; 12584 MW; E17AC86529F36A37 CRC64;
MSQYYELYRR SSIGISLTDA LDDLISQGKI SPQLAMKVLF NFDKSMTEAL AEKVRSRLTF
KGHLDTYRFC DEVWTFIIKN PSFRFDNETV TSNKIRIVAC ATRDSSANR
