T2AG_YEAST
ID T2AG_YEAST Reviewed; 122 AA.
AC P32774; D6VXM9;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 199.
DE RecName: Full=Transcription initiation factor IIA subunit 2;
DE AltName: Full=General transcription factor IIA subunit 2;
DE AltName: Full=TFIIA 13.5 kDa subunit;
DE AltName: Full=Transcription initiation factor IIA small chain;
DE AltName: Full=Transcription initiation factor IIA small subunit;
GN Name=TOA2; OrderedLocusNames=YKL058W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 15-30 AND 40-58.
RX PubMed=1546313; DOI=10.1126/science.1546313;
RA Ranish J.A., Lane W.S., Hahn S.;
RT "Isolation of two genes that encode subunits of the yeast transcription
RT factor IIA.";
RL Science 255:1127-1129(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091862; DOI=10.1002/yea.320100008;
RA Rasmussen S.W.;
RT "Sequence of a 28.6 kb region of yeast chromosome XI includes the FBA1 and
RT TOA2 genes, an open reading frame (ORF) similar to a translationally
RT controlled tumour protein, one ORF containing motifs also found in plant
RT storage proteins and 13 ORFs with weak or no homology to known proteins.";
RL Yeast 10:S63-S68(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP FUNCTION, AND SUBUNIT.
RX PubMed=1918049; DOI=10.1016/s0021-9258(18)55000-6;
RA Ranish J.A., Hahn S.;
RT "The yeast general transcription factor TFIIA is composed of two
RT polypeptide subunits.";
RL J. Biol. Chem. 266:19320-19327(1991).
RN [7]
RP INTERACTION WITH KAP122 AND TOA1, AND SUBCELLULAR LOCATION.
RX PubMed=10525531; DOI=10.1083/jcb.147.2.235;
RA Titov A.A., Blobel G.;
RT "The karyopherin Kap122p/Pdr6p imports both subunits of the transcription
RT factor IIA into the nucleus.";
RL J. Cell Biol. 147:235-246(1999).
RN [8]
RP MUTAGENESIS OF ILE-27 AND LEU-41, AND INTERACTION WITH TAF11.
RX PubMed=11238911; DOI=10.1128/mcb.21.5.1737-1746.2001;
RA Kraemer S.M., Ranallo R.T., Ogg R.C., Stargell L.A.;
RT "TFIIA interacts with TFIID via association with TATA-binding protein and
RT TAF40.";
RL Mol. Cell. Biol. 21:1737-1746(2001).
RN [9]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95 AND SER-102, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [11]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [12]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH TOA1; TBP AND DNA.
RX PubMed=8610010; DOI=10.1038/381127a0;
RA Tan S., Hunziker Y., Sargent D.F., Richmond T.J.;
RT "Crystal structure of a yeast TFIIA/TBP/DNA complex.";
RL Nature 381:127-134(1996).
CC -!- FUNCTION: TFIIA is a component of the transcription machinery of RNA
CC polymerase II and plays an important role in transcriptional
CC activation. TFIIA in a complex with TBP mediates transcriptional
CC activity. {ECO:0000269|PubMed:1918049}.
CC -!- SUBUNIT: TFIIA is a heterodimer composed of the large TOA1 and a small
CC TOA2 subunits. Interacts with TBP. Interacts with TAF11. Interacts with
CC KAP122. {ECO:0000269|PubMed:10525531, ECO:0000269|PubMed:11238911,
CC ECO:0000269|PubMed:1918049, ECO:0000269|PubMed:8610010}.
CC -!- INTERACTION:
CC P32774; P13393: SPT15; NbExp=2; IntAct=EBI-19323, EBI-19129;
CC P32774; P32773: TOA1; NbExp=2; IntAct=EBI-19323, EBI-19316;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10525531}. Nucleus
CC {ECO:0000269|PubMed:10525531}. Note=Imported to nucleus via interaction
CC with KAP122.
CC -!- MISCELLANEOUS: Present with 3910 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the TFIIA subunit 2 family. {ECO:0000305}.
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DR EMBL; M85249; AAA19653.1; -; Unassigned_DNA.
DR EMBL; X75781; CAA53423.1; -; Genomic_DNA.
DR EMBL; Z28058; CAA81895.1; -; Genomic_DNA.
DR EMBL; AY557890; AAS56216.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09099.1; -; Genomic_DNA.
DR PIR; A41810; A41810.
DR RefSeq; NP_012865.1; NM_001179624.1.
DR PDB; 1NH2; X-ray; 1.90 A; D=2-122.
DR PDB; 1RM1; X-ray; 2.50 A; B=1-122.
DR PDB; 1YTF; X-ray; 2.50 A; D=2-122.
DR PDB; 5FMF; EM; 6.00 A; O=1-122.
DR PDB; 5FYW; EM; 4.35 A; V=1-122.
DR PDB; 5FZ5; EM; 8.80 A; V=1-122.
DR PDB; 5OQJ; EM; 4.70 A; V=1-122.
DR PDB; 5OQM; EM; 5.80 A; V=1-122.
DR PDB; 5SVA; EM; 15.30 A; e=1-122.
DR PDB; 6GYK; EM; 5.10 A; V=1-122.
DR PDB; 6GYL; EM; 4.80 A; V=1-122.
DR PDB; 6GYM; EM; 6.70 A; V=1-122.
DR PDB; 7O4I; EM; 3.20 A; V=1-122.
DR PDB; 7O4J; EM; 2.90 A; V=1-122.
DR PDB; 7O72; EM; 3.40 A; V=1-122.
DR PDB; 7O73; EM; 3.40 A; V=1-122.
DR PDB; 7O75; EM; 3.20 A; V=1-122.
DR PDB; 7OH9; EM; 3.00 A; M=1-122.
DR PDB; 7OHA; EM; 2.90 A; M=1-122.
DR PDBsum; 1NH2; -.
DR PDBsum; 1RM1; -.
DR PDBsum; 1YTF; -.
DR PDBsum; 5FMF; -.
DR PDBsum; 5FYW; -.
DR PDBsum; 5FZ5; -.
DR PDBsum; 5OQJ; -.
DR PDBsum; 5OQM; -.
DR PDBsum; 5SVA; -.
DR PDBsum; 6GYK; -.
DR PDBsum; 6GYL; -.
DR PDBsum; 6GYM; -.
DR PDBsum; 7O4I; -.
DR PDBsum; 7O4J; -.
DR PDBsum; 7O72; -.
DR PDBsum; 7O73; -.
DR PDBsum; 7O75; -.
DR PDBsum; 7OH9; -.
DR PDBsum; 7OHA; -.
DR AlphaFoldDB; P32774; -.
DR SMR; P32774; -.
DR BioGRID; 34075; 39.
DR ComplexPortal; CPX-1633; Transcription factor TFIIA complex.
DR DIP; DIP-2348N; -.
DR IntAct; P32774; 9.
DR MINT; P32774; -.
DR STRING; 4932.YKL058W; -.
DR iPTMnet; P32774; -.
DR MaxQB; P32774; -.
DR PaxDb; P32774; -.
DR PRIDE; P32774; -.
DR EnsemblFungi; YKL058W_mRNA; YKL058W; YKL058W.
DR GeneID; 853807; -.
DR KEGG; sce:YKL058W; -.
DR SGD; S000001541; TOA2.
DR VEuPathDB; FungiDB:YKL058W; -.
DR eggNOG; KOG3463; Eukaryota.
DR GeneTree; ENSGT00390000014572; -.
DR HOGENOM; CLU_112964_3_1_1; -.
DR InParanoid; P32774; -.
DR OMA; QYYELYR; -.
DR BioCyc; YEAST:G3O-31857-MON; -.
DR Reactome; R-SCE-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-SCE-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-SCE-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-SCE-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-SCE-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-SCE-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-SCE-9018519; Estrogen-dependent gene expression.
DR EvolutionaryTrace; P32774; -.
DR PRO; PR:P32774; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P32774; protein.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:ComplexPortal.
DR GO; GO:0005672; C:transcription factor TFIIA complex; IDA:SGD.
DR GO; GO:0000979; F:RNA polymerase II core promoter sequence-specific DNA binding; IMP:CAFA.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; IBA:GO_Central.
DR GO; GO:0017025; F:TBP-class protein binding; IPI:CAFA.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:ComplexPortal.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IDA:ComplexPortal.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IDA:SGD.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IDA:SGD.
DR CDD; cd10014; TFIIA_gamma_C; 1.
DR CDD; cd10145; TFIIA_gamma_N; 1.
DR DisProt; DP00009; -.
DR Gene3D; 1.10.287.190; -; 1.
DR Gene3D; 2.30.18.10; -; 1.
DR InterPro; IPR009083; TFIIA_a-hlx.
DR InterPro; IPR009088; TFIIA_b-brl.
DR InterPro; IPR003194; TFIIA_gsu.
DR InterPro; IPR015871; TFIIA_gsu_C.
DR InterPro; IPR015872; TFIIA_gsu_N.
DR PANTHER; PTHR10966; PTHR10966; 1.
DR Pfam; PF02751; TFIIA_gamma_C; 1.
DR Pfam; PF02268; TFIIA_gamma_N; 1.
DR PIRSF; PIRSF009415; Hum_TFIIA_gamma; 1.
DR SUPFAM; SSF50784; SSF50784; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Direct protein sequencing; Nucleus;
KW Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..122
FT /note="Transcription initiation factor IIA subunit 2"
FT /id="PRO_0000194053"
FT MOD_RES 95
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956,
FT ECO:0007744|PubMed:19779198"
FT MOD_RES 102
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MUTAGEN 27
FT /note="I->A,K: Decreases ability to interact with TAF11 and
FT support growth on galactose-containing medium. Unable to
FT support cell viability in a strain deleted for TOA2; when
FT associated with A-69."
FT /evidence="ECO:0000269|PubMed:11238911"
FT MUTAGEN 41
FT /note="L->D: Decreases ability to interact with Toa1 and
FT TAF11, display mutant growth phenotypes and defects in
FT transcription in vivo."
FT /evidence="ECO:0000269|PubMed:11238911"
FT MUTAGEN 69
FT /note="Y->A: Unable to support cell viability in a strain
FT deleted for TOA2; when associated with A-27 or K-27."
FT HELIX 9..12
FT /evidence="ECO:0007829|PDB:1NH2"
FT HELIX 14..28
FT /evidence="ECO:0007829|PDB:1NH2"
FT HELIX 34..54
FT /evidence="ECO:0007829|PDB:1NH2"
FT STRAND 59..72
FT /evidence="ECO:0007829|PDB:1NH2"
FT STRAND 75..88
FT /evidence="ECO:0007829|PDB:1NH2"
FT STRAND 104..119
FT /evidence="ECO:0007829|PDB:1NH2"
SQ SEQUENCE 122 AA; 13458 MW; 5EE90C1BE414F949 CRC64;
MAVPGYYELY RRSTIGNSLV DALDTLISDG RIEASLAMRV LETFDKVVAE TLKDNTQSKL
TVKGNLDTYG FCDDVWTFIV KNCQVTVEDS HRDASQNGSG DSQSVISVDK LRIVACNSKK
SE
