ID   T2A_PARTE               Reviewed;         386 AA.
AC   Q27173; A0CLM9;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 2.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Trichocyst matrix protein T2-A;
DE   AltName: Full=Secretory granule protein T2-A;
DE   AltName: Full=TMP 2-A;
DE   Contains:
DE     RecName: Full=Trichocyst matrix protein T2-A 1;
DE   Contains:
DE     RecName: Full=Trichocyst matrix protein T2-A 2;
DE   Flags: Precursor;
GN   Name=T2A; ORFNames=GSPATT00008245001;
OS   Paramecium tetraurelia.
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC   Oligohymenophorea; Peniculida; Parameciidae; Paramecium.
OX   NCBI_TaxID=5888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Stock d4-2;
RX   PubMed=17086204; DOI=10.1038/nature05230;
RA   Aury J.-M., Jaillon O., Duret L., Noel B., Jubin C., Porcel B.M.,
RA   Segurens B., Daubin V., Anthouard V., Aiach N., Arnaiz O., Billaut A.,
RA   Beisson J., Blanc I., Bouhouche K., Camara F., Duharcourt S., Guigo R.,
RA   Gogendeau D., Katinka M., Keller A.-M., Kissmehl R., Klotz C., Koll F.,
RA   Le Mouel A., Lepere G., Malinsky S., Nowacki M., Nowak J.K., Plattner H.,
RA   Poulain J., Ruiz F., Serrano V., Zagulski M., Dessen P., Betermier M.,
RA   Weissenbach J., Scarpelli C., Schaechter V., Sperling L., Meyer E.,
RA   Cohen J., Wincker P.;
RT   "Global trends of whole-genome duplications revealed by the ciliate
RT   Paramecium tetraurelia.";
RL   Nature 444:171-178(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 49-71.
RC   STRAIN=Stock d4-2;
RX   PubMed=7579685; DOI=10.1091/mbc.6.6.649;
RA   Madeddu L., Gautier M.-C., Vayssie L., Houari A., Sperling L.;
RT   "A large multigene family codes for the polypeptides of the crystalline
RT   trichocyst matrix in Paramecium.";
RL   Mol. Biol. Cell 6:649-659(1995).
RN   [3]
RP   PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=Stock d4-2;
RX   PubMed=7819344; DOI=10.1016/0300-9084(94)90167-8;
RA   Madeddu L., Gautier M.-C., le Caer J.-P., de Loubresse N., Sperling L.;
RT   "Protein processing and morphogenesis of secretory granules in
RT   Paramecium.";
RL   Biochimie 76:329-335(1994).
CC   -!- FUNCTION: Structural protein that crystallize inside the trichocyst
CC       matrix.
CC   -!- SUBCELLULAR LOCATION: Trichocyst. Note=These are architecturally
CC       complex secretory storage granules-docked at the plasma membrane, ready
CC       to rapidly respond to an exocytotic stimulus.
CC   -!- SIMILARITY: Belongs to the TMP family. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=Protein Spotlight; Note=The arsenal of Paramecium
CC       - Issue 3 of October 2000;
CC       URL="https://web.expasy.org/spotlight/back_issues/003";
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DR   EMBL; CT868097; CAK71696.1; -; Genomic_DNA.
DR   EMBL; U27509; AAA92609.1; -; Genomic_DNA.
DR   RefSeq; XP_001439093.1; XM_001439056.1.
DR   AlphaFoldDB; Q27173; -.
DR   SMR; Q27173; -.
DR   EnsemblProtists; CAK71696; CAK71696; GSPATT00008245001.
DR   GeneID; 5024878; -.
DR   KEGG; ptm:GSPATT00008245001; -.
DR   eggNOG; ENOG502SUHG; Eukaryota.
DR   HOGENOM; CLU_062544_0_0_1; -.
DR   InParanoid; Q27173; -.
DR   Proteomes; UP000000600; Partially assembled WGS sequence.
DR   GO; GO:0055039; C:trichocyst; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Coiled coil; Direct protein sequencing; Reference proteome; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..48
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000307846"
FT   CHAIN           49..183
FT                   /note="Trichocyst matrix protein T2-A 1"
FT                   /id="PRO_0000221508"
FT   PROPEP          184..238
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000307847"
FT   CHAIN           239..386
FT                   /note="Trichocyst matrix protein T2-A 2"
FT                   /id="PRO_0000307848"
FT   COILED          51..154
FT                   /evidence="ECO:0000255"
FT   COILED          293..332
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   386 AA;  43207 MW;  82B6BC32D32A574B CRC64;
     MKTVILALAL IVLASSTQAD VIATIKKIDQ SPFGRTLFDT IYLELQTGDP LDRLLSTLTD
     LEDRYVAEQK EDDAKNQEYQ GACTVDISAF DKDLAESNRK KIELEARLEG QLYPQRSILE
     GLVAQKKAEV KGYQKDLDEL DAQRAEEHED FEEKVLEHQE ATAIIAEARR LFADNIEHGS
     FIQKGKATKQ THKFTKEVAS MIQKHFTTSA KKTAKFQHRK GYSKLFKAFA TIASKVEQLA
     DAGAVQKIID LADELLAKIA DSLSLLRFAE DKRVEAYKKS RNFIVISLNV AGSALANATS
     DLASLNDIIA QVEASLDTTE QRIENVSADR HDRFTQCEEA VQDYQDARAA RTSDRDVVSQ
     TIGLVNKELR TLREQLALRQ QAGEEI