ID   T2B1_NEIGO              Reviewed;         350 AA.
AC   Q50973;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 2.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=Type II restriction enzyme NgoBI {ECO:0000303|PubMed:12654995};
DE            Short=R.NgoBI;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease NgoBI;
DE   AltName: Full=R.NgoI {ECO:0000303|PubMed:9628358};
DE   AltName: Full=S.NgoI {ECO:0000303|PubMed:9628358};
DE   AltName: Full=Type-2 restriction enzyme NgoBI;
GN   Name=ngoBIR;
OS   Neisseria gonorrhoeae.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=485;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=WR302;
RX   PubMed=9628358;
RA   Stein D.C., Gunn J.S., Piekarowicz A.;
RT   "Sequence similarities between the genes encoding the S.NgoI and HaeII
RT   restriction/modification systems.";
RL   Biol. Chem. 379:575-578(1998).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-RGCGCY-3'; the cleavage site is unknown.
CC       {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; U42459; AAB03207.2; -; Genomic_DNA.
DR   AlphaFoldDB; Q50973; -.
DR   REBASE; 3133; NgoBI.
DR   PRO; PR:Q50973; -.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR019058; Restrct_endonuc_II_HaeII.
DR   Pfam; PF09554; RE_HaeII; 1.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Restriction system.
FT   CHAIN           1..350
FT                   /note="Type II restriction enzyme NgoBI"
FT                   /id="PRO_0000077344"
SQ   SEQUENCE   350 AA;  40638 MW;  EF3EC3540B55E2EF CRC64;
     MTLEEQQAKE ALDGIIKKSR VHLYKPIQIA EILYHDRCIK QLDFLNLDTY RNQSKRWRDE
     ICRRFLGRIS TSSAKFQDNL FEKNAIPPEK LAVLGTLNRQ SDGGVESYIY KQFFNRFSQM
     SERLAYVGNT DRYSFQLSEF LNLFWLEPGL KRSIDKIYEI VVYALFDALV SELGITVSID
     FPKENLFLWE EYQDFAEKII TMPKNEHLKL PAKIHRVGVT NAADRGLDMW SNFGLAIQVK
     HLSLDEELAE DIVSSISADR IVIVCKKAEQ SVIVSLLTQI GWKSRIQNIV TEDDLISWYE
     KALRGQYPIA EALLENIKTE IMREFPAVNE ANEFLDFAQN RGYDITVTHF