ID   T2B5_NEIGO              Reviewed;         175 AA.
AC   Q50975;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Type II restriction enzyme NgoBV {ECO:0000303|PubMed:12654995};
DE            Short=R.NgoBV;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease NgoBV;
DE   AltName: Full=R.NgoV;
DE   AltName: Full=S.NgoV {ECO:0000303|PubMed:7607490};
DE   AltName: Full=Type-2 restriction enzyme NgoBV;
DE   Flags: Fragment;
GN   Name=ngoBVR; Synonyms=dcrE;
OS   Neisseria gonorrhoeae.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=485;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=WR302;
RX   PubMed=7607490; DOI=10.1016/0378-1119(94)00649-d;
RA   Stein D.C., Gunn J.S., Radlinska M., Piekarowicz A.;
RT   "Restriction and modification systems of Neisseria gonorrhoeae.";
RL   Gene 157:19-22(1995).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-GGNNCC-3'; the cleavage site is unknown.
CC       {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; U43735; AAA86269.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q50975; -.
DR   SMR; Q50975; -.
DR   REBASE; 3134; NgoBV.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR019064; Restrct_endonuc_II_NlaIV.
DR   Pfam; PF09564; RE_NgoBV; 1.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Restriction system.
FT   CHAIN           1..>175
FT                   /note="Type II restriction enzyme NgoBV"
FT                   /id="PRO_0000077345"
FT   NON_TER         175
SQ   SEQUENCE   175 AA;  20257 MW;  786E8EBCCDF08235 CRC64;
     MIKLTAQQIF DKLLDEEKIL SANGQIRFFL GDVDIIVKQK DVVGNIIQEW LGGWLRKREI
     EFDVSTNTQM PPDFFLNKKD RSRELLEVKA FNRNACPGFD IADFKMYSDE SFISPISGCR
     LFNIGYDMDD NGNVTIKDLW LKKVWQITRS MDGWAINFKS KKAWCIKSAR VFGTA