ID   T2B6_BACSF              Reviewed;         174 AA.
AC   P43419;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Type II restriction enzyme Bsp6I {ECO:0000303|PubMed:12654995};
DE            Short=R.Bsp6I {ECO:0000303|PubMed:7607501};
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease Bsp6I;
DE   AltName: Full=Type-2 restriction enzyme Bsp6I;
GN   Name=bsp6IR {ECO:0000303|PubMed:7607501};
OS   Bacillus sp. (strain RFL6).
OG   Plasmid pXH13.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=72577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=RFL6;
RX   PubMed=7607501; DOI=10.1016/0378-1119(94)00795-t;
RA   Lubys A., Janulaitis A.;
RT   "Cloning and analysis of the plasmid-borne genes encoding the Bsp6I
RT   restriction and modification enzymes.";
RL   Gene 157:25-29(1995).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-GCNGC-3' and cleaves after C-2.
CC       {ECO:0000303|PubMed:12654995, ECO:0000305|PubMed:7607501}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; X81638; CAA57292.1; -; Genomic_DNA.
DR   PIR; I40137; I40137.
DR   AlphaFoldDB; P43419; -.
DR   REBASE; 485; Bsp6I.
DR   PRO; PR:P43419; -.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR019037; Restrct_endonuc_II_Bsp6I.
DR   Pfam; PF09504; RE_Bsp6I; 1.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Plasmid; Restriction system.
FT   CHAIN           1..174
FT                   /note="Type II restriction enzyme Bsp6I"
FT                   /id="PRO_0000077287"
SQ   SEQUENCE   174 AA;  19905 MW;  9A5DC728F04DCEC6 CRC64;
     MALHDFVKID KSRFEDAVKA YFLWKELNAL IKNSHNRGIN FPETISETLL CAAMGFELNR
     GTGGDAREPK TQAIIESKAT SNWDRDTTSF SPSENFDALY FLRLNQRDDE LYIYNTGINS
     DDLKSIQVNK TQTLGDQQAQ GRRPRFSVIK FIIEANDIEP VAKVELRTKK IIKL