ID   T2BB_BACIU              Reviewed;         316 AA.
AC   P33562;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Type II restriction enzyme BsuBI {ECO:0000303|PubMed:12654995};
DE            Short=R.BsuBI;
DE            EC=3.1.21.4 {ECO:0000269|PubMed:1480472};
DE   AltName: Full=Endonuclease BsuBI;
DE   AltName: Full=Type-2 restriction enzyme BsuBI;
GN   Name=hsdBR; Synonyms=hsrB;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=ISB8;
RX   PubMed=1480472; DOI=10.1093/nar/20.24.6517;
RA   Xu G.-L., Kapfer W., Walter J., Trautner T.A.;
RT   "BsuBI -- an isospecific restriction and modification system of PstI:
RT   characterization of the BsuBI genes and enzymes.";
RL   Nucleic Acids Res. 20:6517-6523(1992).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-CTGCAG-3' and cleaves after A-5.
CC       {ECO:0000269|PubMed:1480472, ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC         Evidence={ECO:0000269|PubMed:1480472};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the BsuBI/PstI type II restriction endonuclease
CC       family. {ECO:0000305}.
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DR   EMBL; L01541; AAA18170.1; -; Genomic_DNA.
DR   PIR; S35516; S35516.
DR   AlphaFoldDB; P33562; -.
DR   SMR; P33562; -.
DR   REBASE; 616; BsuBI.
DR   PRO; PR:P33562; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1820; -; 1.
DR   Gene3D; 3.40.1350.80; -; 1.
DR   InterPro; IPR041963; BsuBI/PstI_C_sf.
DR   InterPro; IPR041454; BsuBI/PstI_N.
DR   InterPro; IPR041962; BsuBI/PstI_N_sf.
DR   InterPro; IPR009528; Restrct_endonuc_II_BsuBI_C.
DR   Pfam; PF06616; BsuBI_PstI_RE; 1.
DR   Pfam; PF17728; BsuBI_PstI_RE_N; 1.
PE   1: Evidence at protein level;
KW   Endonuclease; Hydrolase; Magnesium; Nuclease; Restriction system.
FT   CHAIN           1..316
FT                   /note="Type II restriction enzyme BsuBI"
FT                   /id="PRO_0000077290"
SQ   SEQUENCE   316 AA;  36197 MW;  B1C2E45851E7F3C9 CRC64;
     MTEGMHSNVK EAIKILKELG LPKGQQNERS ALCLLSLMNI TQDKTWSEAE SPLIGITPMM
     EFCRINYGKE YAPNSRETFR RFTMHQFVDA GIALYNPDKP TRPVNSPKAV YQIEAETLEL
     IKCYNTEEWS ELLARYLSNR QTLVERYAKE RQQNKIPVQI AEGKEIYITP GEHSELIKAI
     IEEFAPRYVP GGRLIYAGDT GEKMGYFDEE LLRQLGVVID SHGKMPDVVI YFPEKKWLLL
     IESVTSHGPV DHKRHEELAK LFNGSTAGIV YVTAFPNRSL MARYLNNISW ETEVWVADAP
     SHLIHFNGVR FLGPYE