ID   T2C1_CITFR              Reviewed;         355 AA.
AC   Q04852;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Type II restriction enzyme CfrBI {ECO:0000303|PubMed:12654995};
DE            Short=R.CfrBI {ECO:0000303|PubMed:8335262};
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease CfrBI;
DE   AltName: Full=Type-2 restriction enzyme CfrBI;
GN   Name=cfrBIR {ECO:0000303|PubMed:8335262};
OS   Citrobacter freundii.
OG   Plasmid pZE8.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
OX   NCBI_TaxID=546;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RC   STRAIN=4111;
RX   PubMed=8335262; DOI=10.1016/0378-1119(93)90698-3;
RA   Zakharova M.V., Kravetz A.N., Beletzkaja I.V., Repyk A.V., Solonin A.S.;
RT   "Cloning and sequences of the genes encoding the CfrBI restriction-
RT   modification system from Citrobacter freundii.";
RL   Gene 129:77-81(1993).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-CCWWGG-3' and cleaves after C-1.
CC       {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC   -!- DISRUPTION PHENOTYPE: Loss of restriction enzyme activity.
CC       {ECO:0000269|PubMed:8335262}.
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DR   EMBL; X57945; CAA41013.1; -; Genomic_DNA.
DR   PIR; JN0746; JN0746.
DR   PIR; S28523; S28523.
DR   AlphaFoldDB; Q04852; -.
DR   REBASE; 2422; CfrBI.
DR   BRENDA; 3.1.21.4; 1398.
DR   PRO; PR:Q04852; -.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR019042; Restrct_endonuc_II_CfrBI.
DR   Pfam; PF09516; RE_CfrBI; 1.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Plasmid; Restriction system.
FT   CHAIN           1..355
FT                   /note="Type II restriction enzyme CfrBI"
FT                   /id="PRO_0000077296"
SQ   SEQUENCE   355 AA;  40413 MW;  0FDF4014F95C496A CRC64;
     MNFKDKNCFP NELIALAKIS KNDVLDKFGT DVFKKVVYDV LTGKNVREFT EILTRTRLLE
     SNLSFFDFFV DKMKEGITPK QLYLYAKNAL SNKSYVKSNQ PVLEWMVMMT NKQTQNVLRD
     EHGDGFDRLA LRTQEEILKI KNGYEDKIGE ISIGGQKVSL EDFCYIILSL GSQTLTIRGS
     EKSLHGKYFE KLILGSLFTI MGFEYKEKIE EGLNAKCFTL STRADDRESD ATLVFNGKAI
     RVDIGFIGRG NTEISLDKVS RFRRMDDIGG VMHNISTMVI VDVIGDRSRI VNMAEEIDGK
     VVAMSDPYWV AKVSSYISSK LNVDDLLEDK PQLKDIQSFI SDALENVDLE KYIKL