ID   T2C1_PBCVI              Reviewed;         358 AA.
AC   P52283;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   23-FEB-2022, sequence version 2.
DT   03-AUG-2022, entry version 53.
DE   RecName: Full=Type II restriction enzyme CviJI {ECO:0000303|PubMed:12654995};
DE            Short=R.CviJI {ECO:0000303|PubMed:7607522};
DE            EC=3.1.21.4 {ECO:0000269|PubMed:8692682};
DE   AltName: Full=Endonuclease CviJI;
DE   AltName: Full=Type-2 restriction enzyme CviJI;
GN   Name=CVJIR {ECO:0000303|PubMed:7607522};
OS   Paramecium bursaria Chlorella virus IL3A (PBCV-IL3A).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes;
OC   Algavirales; Phycodnaviridae; Chlorovirus.
OX   NCBI_TaxID=46019;
OH   NCBI_TaxID=3071; Chlorella.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7607522; DOI=10.1016/0378-1119(94)00564-9;
RA   Skowron P.M., Swaminathan N., McMaster K., George D., van Etten J.L.,
RA   Mead D.A.;
RT   "Cloning and applications of the two/three-base restriction endonuclease
RT   R.CviJI from IL-3A virus-infected Chlorella.";
RL   Gene 157:37-41(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 81-95, FUNCTION, AND
RP   CATALYTIC ACTIVITY.
RX   PubMed=8692682; DOI=10.1093/nar/24.13.2463;
RA   Swaminathan N., Mead D.A., McMaster K., George D., van Etten J.L.,
RA   Skowron P.M.;
RT   "Molecular cloning of the three base restriction endonuclease R.CviJI from
RT   eukaryotic Chlorella virus IL-3A.";
RL   Nucleic Acids Res. 24:2463-2469(1996).
RN   [3]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-RGCY-3' and cleaves after G-2. In the presence of
CC       ATP, there is a relaxation of its specificity and it can cleave 5'-
CC       RGCN-3' and 5'-YGCY-3', but not 5'-YGCR-3' (R.CviJI* activity).
CC       {ECO:0000269|PubMed:8692682, ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC         Evidence={ECO:0000269|PubMed:8692682};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC   -!- CAUTION: It is uncertain whether Met-1 or Val-81 is the initiator; upon
CC       expression in E.coli the protein is 32.5 kDa and starts at Val-81.
CC       Recombinant enzyme has no R.CviJI* activity, whereas viral-produced
CC       enzyme is larger than 32.5 kDa and does have R.CviJI* activity,
CC       suggesting Met-1 is the correct initiator when grown in Chlorella
CC       strain NC64A. {ECO:0000269|PubMed:8692682}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC55064.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; U09001; AAC55064.1; ALT_INIT; Genomic_DNA.
DR   REBASE; 760; CviJI.
DR   PRO; PR:P52283; -.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Endonuclease; Hydrolase; Magnesium; Nuclease;
KW   Restriction system.
FT   CHAIN           1..358
FT                   /note="Type II restriction enzyme CviJI"
FT                   /id="PRO_0000077298"
SQ   SEQUENCE   358 AA;  41468 MW;  6AD5C5C72EDD3B2D CRC64;
     MDIRRKRFTI EGAKRIILEK KRLEEKKRIA EEKKRIALIE KQRIAEEKKR IAEEKKRFAL
     EEKKRIAEEK KRIAEEKKRI VEEKKRLALI EKQRIAEEKI ASGRKIRKRI STNATKHERE
     FVKVINSMFV GPATFVFVDI KGNKSREIHN VVRFRQLQGS KAKSPTAYVD REYNKPKADI
     AAVDITGKDV AWISHKASEG YQQYLKISGK NLKFTGKELE EVLSFKRKVV SMAPVSKIWP
     ANKTVWSPIK SNLIKNQAIF GFDYGKKPGR DNVDIIGQGR PIITKRGSIL YLTFTGFSAL
     NGHLENFTGK HEPVFYVRTE RSSSGRSITT VVNGVTYKNL RFFIHPYNFV SSKTQRIM