ID   T2C1_RHOHA              Reviewed;         270 AA.
AC   P42827;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Type II restriction enzyme CeqI {ECO:0000303|PubMed:12654995};
DE            Short=R.CeqI;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease CeqI;
DE   AltName: Full=Type-2 restriction enzyme CeqI;
GN   Name=ceqIR; Synonyms=rceQI;
OS   Rhodococcus hoagii (Corynebacterium equii).
OC   Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Rhodococcus.
OX   NCBI_TaxID=43767;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9304804; DOI=10.1016/s1357-2725(97)00029-0;
RA   Izsvak Z., Jobbagy Z., Takacs I., Duda E.;
RT   "Cloning and characterization of the genes of the CeqI restriction-
RT   modification system.";
RL   Int. J. Biochem. Cell Biol. 29:895-900(1997).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-GATATC-3' and cleaves after T-3.
CC       {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; Z34099; CAA84005.1; -; Genomic_DNA.
DR   AlphaFoldDB; P42827; -.
DR   PRO; PR:P42827; -.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Restriction system.
FT   CHAIN           1..270
FT                   /note="Type II restriction enzyme CeqI"
FT                   /id="PRO_0000077293"
SQ   SEQUENCE   270 AA;  31674 MW;  4D9EE9D689669394 CRC64;
     MRTKNVCTFQ LILFPKVRSY IPLPLILKRI EITKNCISTR GETTKTSVTE KLSEEKTVVV
     EPVFGFLKAH FHSTRFSEEA TKRRKRKWAL LVVILRKYTA RNSKQKRTKK TRQKGVRSSF
     IDERTPFSFI FWLVLFRPLF NVLPCSAQSL FVQEFFITSP CLVCPAFPLE QNVSAFAPST
     NGTKTKQMIT ITPMIRFIVV QLLTLRFSYR TVKGISPLRR RAWFFLWFNR SKCFCVGSIH
     KRNKNKADKQ YQTEYSFHCC STPIIFILVR