T2C2_HERAU
ID T2C2_HERAU Reviewed; 273 AA.
AC P25259;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 1.
DT 25-MAY-2022, entry version 64.
DE RecName: Full=Type II restriction enzyme HgiCII {ECO:0000303|PubMed:12654995};
DE Short=R.HgiCII {ECO:0000303|PubMed:1644308};
DE EC=3.1.21.4;
DE AltName: Full=Endonuclease HgiCII;
DE AltName: Full=Type-2 restriction enzyme HgiCII;
GN Name=hgiCIIR {ECO:0000303|PubMed:1644308};
OS Herpetosiphon aurantiacus (Herpetosiphon giganteus).
OC Bacteria; Chloroflexi; Chloroflexia; Herpetosiphonales; Herpetosiphonaceae;
OC Herpetosiphon.
OX NCBI_TaxID=65;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=HPG9;
RX PubMed=1644308; DOI=10.1016/0378-1119(92)90484-7;
RA Erdmann D., Horst G., Duesterhoeft A., Kroeger M.;
RT "Stepwise cloning and genetic organization of the seemingly unclonable
RT HgiCII restriction-modification system from Herpetosiphon giganteus strain
RT Hpg9, using PCR technique.";
RL Gene 117:15-22(1992).
RN [2]
RP DISCUSSION OF SEQUENCE.
RX PubMed=7607523; DOI=10.1016/0378-1119(94)00779-r;
RA Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S.,
RA Meyer-Rogge S., Moestl D.;
RT "Organization and gene expression within restriction-modification systems
RT of Herpetosiphon giganteus.";
RL Gene 157:43-47(1995).
RN [3]
RP NOMENCLATURE, AND SUBTYPE.
RX PubMed=12654995; DOI=10.1093/nar/gkg274;
RA Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT endonucleases and their genes.";
RL Nucleic Acids Res. 31:1805-1812(2003).
CC -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC stranded sequence 5'-GGWCC-3' and cleaves after G-1.
CC {ECO:0000303|PubMed:12654995, ECO:0000305|PubMed:1644308}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC -!- SIMILARITY: Belongs to the TdeIII type II restriction endonuclease
CC family. {ECO:0000305}.
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DR EMBL; X55139; CAA38936.1; -; Genomic_DNA.
DR PIR; JC1166; JC1166.
DR AlphaFoldDB; P25259; -.
DR REBASE; 1100; HgiCII.
DR PRO; PR:P25259; -.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR InterPro; IPR019045; Restrct_endonuc_II_TdeIII.
DR Pfam; PF09520; RE_TdeIII; 1.
PE 3: Inferred from homology;
KW Endonuclease; Hydrolase; Nuclease; Restriction system.
FT CHAIN 1..273
FT /note="Type II restriction enzyme HgiCII"
FT /id="PRO_0000077312"
SQ SEQUENCE 273 AA; 31056 MW; 0403DF0B9F564619 CRC64;
MSINPITRNK IKDYLNGFID QQLAVYSQRN LREFHDVDSY LAAISSDGDL KPFHASIIPS
AIMRLNRFER SLSTGLGSTF EECARVIALD HHAVAIRSYD IHTSLDQAVW ASIDLLISNI
DRNNQRQIPS ITEMLEKLQS IALTGIAENH VVRADLYVQR HDGSELFFEI KSPKPNKGQC
LEVMQRLLRI YAIKQNSTLP THAFYAMAYN PWGANRASYT YSIVKKYTDF TNAVVIGQEF
WSLIGESSTY TELLEIYREV GLSKSSEITK KLL
