ID   T2C2_PBCV1              Reviewed;         342 AA.
AC   P31117;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   23-FEB-2022, entry version 76.
DE   RecName: Full=Type II restriction enzyme CviAII {ECO:0000303|PubMed:12654995};
DE            Short=R.CviAII {ECO:0000303|PubMed:1437552};
DE            EC=3.1.21.4 {ECO:0000269|PubMed:1437552};
DE   AltName: Full=Endonuclease CviAII;
DE   AltName: Full=Type-2 restriction enzyme CviAII;
GN   Name=CVIAIIR; OrderedLocusNames=A252R;
OS   Paramecium bursaria Chlorella virus 1 (PBCV-1).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes;
OC   Algavirales; Phycodnaviridae; Chlorovirus.
OX   NCBI_TaxID=10506;
OH   NCBI_TaxID=114055; Chlorella.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, AND
RP   BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=1437552; DOI=10.1093/nar/20.20.5351;
RA   Zhang Y., Nelson M., Nietfeldt J.W., Burbank D.E., van Etten J.L.;
RT   "Characterization of Chlorella virus PBCV-1 CviAII restriction and
RT   modification system.";
RL   Nucleic Acids Res. 20:5351-5356(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8614977; DOI=10.1006/viro.1996.0038;
RA   Lu Z., Li Y., Que Q., Kutish G.F., Rock D.L., van Etten J.L.;
RT   "Analysis of 94 kb of the chlorella virus PBCV-1 330-kb genome: map
RT   positions 88 to 182.";
RL   Virology 216:102-123(1996).
RN   [3]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-CATG-3' and cleaves after C-1.
CC       {ECO:0000269|PubMed:1437552, ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC         Evidence={ECO:0000269|PubMed:1437552};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 8.5-9.0. {ECO:0000269|PubMed:1437552};
CC       Temperature dependence:
CC         Optimum temperature is 23 degrees Celsius.
CC         {ECO:0000269|PubMed:1437552};
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DR   EMBL; M86639; AAB92382.1; -; Genomic_DNA.
DR   EMBL; JF411744; AAC96620.1; -; Genomic_DNA.
DR   PIR; S35443; S27902.
DR   RefSeq; NP_048602.2; NC_000852.5.
DR   GeneID; 918443; -.
DR   KEGG; vg:918443; -.
DR   PRO; PR:P31117; -.
DR   Proteomes; UP000000862; Genome.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Endonuclease; Hydrolase; Nuclease; Reference proteome; Restriction system.
FT   CHAIN           1..342
FT                   /note="Type II restriction enzyme CviAII"
FT                   /id="PRO_0000077297"
SQ   SEQUENCE   342 AA;  39928 MW;  D553D28BD6300522 CRC64;
     MTQKILNPVT GRFVKVDGST GKKIKTGNIY DVNNILSSKL TKKIFDKIRR NDLAKEERET
     QQLTKKISKG IFDKIRSENK KYEKNHQKLT KKMVLDIFSK IYKEDSRKSK TQCVVSEKKD
     NGGVLLTEDL GKIFEKSICM LYDTPYIGPY KYGNEKPMLL KTRLHKLLDF FPELTHTAAG
     GALHDFTTKN SRYLSAKTSK KKDGKVAPQK IGQPTKKKFL EFFNLPPDTS NDDIKLFIKK
     NIVRILDEYF KYTFDDTIIY YNEVNNIIML VKTLKKVKFD PNLIEFGCNK PGKSWKESTI
     LFYNNKRLGE FQIHTSRSCI KFRWFFENIL LLFPDNFEVT IL