ID   T2D1_NEIMC              Reviewed;         351 AA.
AC   Q9RLM3;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 47.
DE   RecName: Full=Type II restriction enzyme NmeDI {ECO:0000303|PubMed:12654995};
DE            Short=R.NmeDI;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease NmeDI;
DE   AltName: Full=Type-2 restriction enzyme NmeDI;
GN   Name=nmeDIRP;
OS   Neisseria meningitidis serogroup C.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=135720;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=2120 / Serogroup C / Serotype NT;
RX   PubMed=10671450; DOI=10.1128/jb.182.5.1296-1303.2000;
RA   Claus H., Friedrich A., Frosch M., Vogel U.;
RT   "Differential distribution of novel restriction-modification systems in
RT   clonal lineages of Neisseria meningitidis.";
RL   J. Bacteriol. 182:1296-1303(2000).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-N(12)RCCGGYN(12)-3' and cleaves on both sides of
CC       the recognition sequence. {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; AJ238948; CAB59898.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9RLM3; -.
DR   REBASE; 4187; NmeDI.
DR   BRENDA; 3.1.21.4; 3593.
DR   PRO; PR:Q9RLM3; -.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Restriction system.
FT   CHAIN           1..351
FT                   /note="Type II restriction enzyme NmeDI"
FT                   /id="PRO_0000077351"
SQ   SEQUENCE   351 AA;  39712 MW;  E65509848A6FEB07 CRC64;
     MSGQKLTAAN LVSAIDSLKK NVNYNYVNSS NTHTIQIVHV EKPEGPIKIK RGDTETSISK
     QAIWRLADAL STGEPVNVDR VFGASYNFRS ALEALLAHTP EIYWCKLQRI QPGLNKSEVV
     EGHKHIIWLP DRPHEKGVMK EYETDVVISE IPSNNVVYDA LSLPSTHSEI DIDVKRRHVQ
     IQIALSAIGF QLGFRTWIAR NDQGISYGDK KIADLDGIVQ KIESEKLISS FDGAVQAAMN
     IDCIWFRNGK LMPAVMEVEH STGVRSGLAR MKQLKDLLPP YANTRWVIVA PDEDRDKVFK
     EANVPMFKNL DTQYFPYSAV EELYSLCIRR KLTNKAVNEE FLDCFMEKCV M