T2D1_STRR6
ID T2D1_STRR6 Reviewed; 254 AA.
AC P0A460; P09356;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 73.
DE RecName: Full=Type II Methyl-directed restriction enzyme DpnI {ECO:0000303|PubMed:12654995};
DE Short=R.DpnI;
DE EC=3.1.21.4;
DE AltName: Full=Endonuclease DpnI;
DE AltName: Full=Type-2 restriction enzyme DpnI;
GN Name=dpnC; OrderedLocusNames=spr1665;
OS Streptococcus pneumoniae (strain ATCC BAA-255 / R6).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=171101;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-255 / R6;
RX PubMed=11544234; DOI=10.1128/jb.183.19.5709-5717.2001;
RA Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S.,
RA DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C.,
RA Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J.,
RA Lee L.N., Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M.,
RA McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B.,
RA Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y.,
RA Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R.,
RA Rosteck P.R. Jr., Skatrud P.L., Glass J.I.;
RT "Genome of the bacterium Streptococcus pneumoniae strain R6.";
RL J. Bacteriol. 183:5709-5717(2001).
RN [2]
RP NOMENCLATURE, AND SUBTYPES.
RX PubMed=12654995; DOI=10.1093/nar/gkg274;
RA Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT endonucleases and their genes.";
RL Nucleic Acids Res. 31:1805-1812(2003).
CC -!- FUNCTION: An M and P subtype restriction enzyme that recognizes the
CC double-stranded and methylated sequence 5'-G(Me)ATC-3' and cleaves
CC after A-2. {ECO:0000250|UniProtKB:P0A459, ECO:0000303|PubMed:12654995}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC Evidence={ECO:0000250|UniProtKB:P0A459};
CC -!- SIMILARITY: Belongs to the DpnI type II restriction endonuclease
CC family. {ECO:0000305}.
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DR EMBL; AE007317; AAL00468.1; -; Genomic_DNA.
DR PIR; G98079; G98079.
DR RefSeq; NP_359257.1; NC_003098.1.
DR RefSeq; WP_000418960.1; NC_003098.1.
DR PDB; 4ESJ; X-ray; 2.05 A; A/B=1-254.
DR PDBsum; 4ESJ; -.
DR AlphaFoldDB; P0A460; -.
DR SMR; P0A460; -.
DR STRING; 171101.spr1665; -.
DR REBASE; 5630; SpnRORF1665P.
DR EnsemblBacteria; AAL00468; AAL00468; spr1665.
DR GeneID; 60234182; -.
DR KEGG; spr:spr1665; -.
DR PATRIC; fig|171101.6.peg.1799; -.
DR eggNOG; ENOG502Z8N2; Bacteria.
DR HOGENOM; CLU_095681_0_0_9; -.
DR OMA; HHIMCSI; -.
DR PRO; PR:P0A460; -.
DR Proteomes; UP000000586; Chromosome.
DR GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.40.210.30; -; 1.
DR InterPro; IPR010324; DRP.
DR InterPro; IPR041368; DRP_C.
DR InterPro; IPR043025; DRP_PD-(D/E)XK_dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF06044; DpnI; 1.
DR Pfam; PF17726; DpnI_C; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW Restriction system.
FT CHAIN 1..254
FT /note="Type II Methyl-directed restriction enzyme DpnI"
FT /id="PRO_0000077301"
FT HELIX 7..12
FT /evidence="ECO:0007829|PDB:4ESJ"
FT TURN 13..15
FT /evidence="ECO:0007829|PDB:4ESJ"
FT HELIX 19..31
FT /evidence="ECO:0007829|PDB:4ESJ"
FT TURN 35..37
FT /evidence="ECO:0007829|PDB:4ESJ"
FT STRAND 53..55
FT /evidence="ECO:0007829|PDB:4ESJ"
FT TURN 57..59
FT /evidence="ECO:0007829|PDB:4ESJ"
FT STRAND 62..71
FT /evidence="ECO:0007829|PDB:4ESJ"
FT STRAND 74..80
FT /evidence="ECO:0007829|PDB:4ESJ"
FT HELIX 81..89
FT /evidence="ECO:0007829|PDB:4ESJ"
FT STRAND 95..101
FT /evidence="ECO:0007829|PDB:4ESJ"
FT STRAND 105..113
FT /evidence="ECO:0007829|PDB:4ESJ"
FT HELIX 115..117
FT /evidence="ECO:0007829|PDB:4ESJ"
FT HELIX 120..122
FT /evidence="ECO:0007829|PDB:4ESJ"
FT STRAND 123..129
FT /evidence="ECO:0007829|PDB:4ESJ"
FT STRAND 138..144
FT /evidence="ECO:0007829|PDB:4ESJ"
FT HELIX 145..147
FT /evidence="ECO:0007829|PDB:4ESJ"
FT HELIX 150..152
FT /evidence="ECO:0007829|PDB:4ESJ"
FT STRAND 154..158
FT /evidence="ECO:0007829|PDB:4ESJ"
FT HELIX 165..174
FT /evidence="ECO:0007829|PDB:4ESJ"
FT HELIX 175..180
FT /evidence="ECO:0007829|PDB:4ESJ"
FT HELIX 183..198
FT /evidence="ECO:0007829|PDB:4ESJ"
FT STRAND 201..205
FT /evidence="ECO:0007829|PDB:4ESJ"
FT HELIX 206..210
FT /evidence="ECO:0007829|PDB:4ESJ"
FT HELIX 213..219
FT /evidence="ECO:0007829|PDB:4ESJ"
FT HELIX 226..239
FT /evidence="ECO:0007829|PDB:4ESJ"
FT STRAND 242..245
FT /evidence="ECO:0007829|PDB:4ESJ"
FT STRAND 250..253
FT /evidence="ECO:0007829|PDB:4ESJ"
SQ SEQUENCE 254 AA; 29785 MW; 7FFFFE14BB3EFEDB CRC64;
MELHFNLELV ETYKSNSQKA RILTEDWVYR QSYCPNCGNN PLNHFENNRP VADFYCNHCS
EEFELKSKKG NFSSTINDGA YATMMKRVQA DNNPNFFFLT YTKNFEVNNF LVLPKQFVTP
KSIIQRKPLA PTARRAGWIG CNIDLSQVPS KGRIFLVQDG QVRDPEKVTK EFKQGLFLRK
SSLSSRGWTI EILNCIDKIE GSEFTLEDMY RFESDLKNIF VKNNHIKEKI RQQLQILRDK
EIIEFKGRGK YRKL
