ID   T2D2_HERAU              Reviewed;         611 AA.
AC   P25280;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Putative type II restriction enzyme HgiDII {ECO:0000303|PubMed:12654995};
DE            Short=R.HgiDII;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease HgiDII;
DE   AltName: Full=ORF68 {ECO:0000303|PubMed:1937045};
OS   Herpetosiphon aurantiacus (Herpetosiphon giganteus).
OC   Bacteria; Chloroflexi; Chloroflexia; Herpetosiphonales; Herpetosiphonaceae;
OC   Herpetosiphon.
OX   NCBI_TaxID=65;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=HPA2;
RX   PubMed=1937045; DOI=10.1016/0378-1119(91)90569-w;
RA   Duesterhoeft A., Kroeger M.;
RT   "Cloning, sequence and characterization of m5C-methyltransferase-encoding
RT   gene, hgiDIIM (GTCGAC), from Herpetosiphon giganteus strain Hpa2.";
RL   Gene 106:87-92(1991).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: According to REBASE this is a P subtype restriction enzyme
CC       that recognizes the double-stranded sequence 5'-GTCGAC-3' and cleaves
CC       after G-1 (PubMed:12654995). No restriction activity was detected upon
CC       overexpressing this protein in E.coli (PubMed:1937045).
CC       {ECO:0000269|PubMed:1937045, ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; X55141; CAA38942.1; -; Genomic_DNA.
DR   PIR; JT0592; JT0592.
DR   AlphaFoldDB; P25280; -.
DR   REBASE; 1103; HgiDII.
DR   PRIDE; P25280; -.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   SUPFAM; SSF55874; SSF55874; 1.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Repeat; Restriction system.
FT   CHAIN           1..611
FT                   /note="Putative type II restriction enzyme HgiDII"
FT                   /id="PRO_0000066188"
FT   REPEAT          382..392
FT                   /note="1"
FT   REPEAT          393..403
FT                   /note="2"
FT   REPEAT          404..409
FT                   /note="3; truncated"
FT   REGION          355..434
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          382..403
FT                   /note="2.5 X 11 AA tandem repeats"
FT   COMPBIAS        369..413
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   611 AA;  68354 MW;  E7BCC060EABE554A CRC64;
     MSDSGYELSF SLNTLNHLSE GLYSNILRFY LKLVANAWDA DATEVSINIR QDEIVIQDNG
     IGMSIEDANT KFLRIGHQKR EDSANTISGR HVMGRKGIGI LAIFGIANIA EVYSCKDGVP
     HGFIIHKGDI ERGISSDVTL YRPSSVPQEA LSIESGTKII LREIKSSIGN AEKTLRTDLA
     RRFTIINNNC NFSVIINNTP INDNDRDYLN KVQFLWYLGG ESSKYANFFT KLKKSFEITN
     LGDGMSGMTV KGWIGTVYRP SDIPNEQPYH IYFAHGKMIQ EDILIDITDA GLYRQYIIGE
     IEADFMDSDD EDDIITSNRQ RIKQTDPRYL KLLEYVKIDI MRVIASTWTK LRKEYPSNPK
     KEEVNDSSLS KDSNSSEKEN TNASSDSSTA TENASSDSST ATENASSETN DGEVEDNSFF
     DDDIPEPSPP PKQEITTAFR EMKNLVKNSN IPNQMKNIIL YDIQQAAYAY KGTSFKACIV
     MLGAILEGVM LGTIQRTDVL EYLIALPQVP KPLSDLGPRN PKFADRTVLA QYIGTTFSFQ
     DCKEIIELCV QGTNKLGVDI LQTVRNSIHP GSVLKDMKQL ARFNHQSAVG YIAKLHEIIN
     LVILWNPPSI P