BPE_ARATH
ID BPE_ARATH Reviewed; 343 AA.
AC Q0JXE7; Q84JL1; Q8LCW3; Q9LQ51; Q9SLT6;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Transcription factor BPE;
DE AltName: Full=Basic helix-loop-helix protein 31;
DE Short=AtbHLH31;
DE Short=bHLH 31;
DE AltName: Full=Protein BIG PETAL;
DE AltName: Full=Transcription factor EN 88;
DE AltName: Full=bHLH transcription factor bHLH031;
GN Name=BPE; Synonyms=BHLH31, EN88, ZCW32; OrderedLocusNames=At1g59640;
GN ORFNames=T30E16.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, ALTERNATIVE
RP SPLICING, INDUCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=16902407; DOI=10.1038/sj.emboj.7601270;
RA Szecsi J., Joly C., Bordji K., Varaud E., Cock J.M., Dumas C.,
RA Bendahmane M.;
RT "BIGPETALp, a bHLH transcription factor is involved in the control of
RT Arabidopsis petal size.";
RL EMBO J. 25:3912-3920(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-318 (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=10548732; DOI=10.1016/s0378-1119(99)00403-5;
RA Kato A., Suzuki M., Kuwahara A., Ooe H., Higano-Inaba K., Komeda Y.;
RT "Isolation and analysis of cDNA within a 300 kb Arabidopsis thaliana
RT genomic region located around the 100 map unit of chromosome 1.";
RL Gene 239:309-316(1999).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [8]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [9]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
CC -!- FUNCTION: Involved in the control of petal size, by interfering with
CC postmitotic cell expansion to limit final petal cell size.
CC {ECO:0000269|PubMed:16902407}.
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=BPEp;
CC IsoId=Q0JXE7-1; Sequence=Displayed;
CC Name=2; Synonyms=BPEub;
CC IsoId=Q0JXE7-2; Sequence=VSP_036110, VSP_036111, VSP_036112;
CC -!- TISSUE SPECIFICITY: [Isoform 1]: Specifically expressed in flowers,
CC mostly in petals, inflorescence and flower buds.
CC {ECO:0000269|PubMed:16902407}.
CC -!- TISSUE SPECIFICITY: [Isoform 2]: Expressed ubiquitously (leaves,
CC flowers and stems). {ECO:0000269|PubMed:16902407}.
CC -!- INDUCTION: [Isoform 1]: Up-regulated by PI/AP3, SEP2, SEP3 and AP1, but
CC repressed by AG. {ECO:0000269|PubMed:16902407}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79771.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AM269753; CAK32498.1; -; mRNA.
DR EMBL; AM269754; CAK32499.1; -; mRNA.
DR EMBL; AC009317; AAF79771.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33597.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33598.1; -; Genomic_DNA.
DR EMBL; BT003964; AAO42009.1; -; mRNA.
DR EMBL; BT005064; AAO50597.1; -; mRNA.
DR EMBL; AY086373; AAM64440.1; -; mRNA.
DR EMBL; AB028232; BAA87957.1; -; mRNA.
DR PIR; B96620; B96620.
DR PIR; T52428; T52428.
DR RefSeq; NP_564749.1; NM_104657.4. [Q0JXE7-2]
DR RefSeq; NP_849829.1; NM_179498.3. [Q0JXE7-1]
DR AlphaFoldDB; Q0JXE7; -.
DR BioGRID; 27479; 3.
DR IntAct; Q0JXE7; 2.
DR STRING; 3702.AT1G59640.2; -.
DR PaxDb; Q0JXE7; -.
DR PRIDE; Q0JXE7; -.
DR ProteomicsDB; 222818; -. [Q0JXE7-1]
DR EnsemblPlants; AT1G59640.1; AT1G59640.1; AT1G59640. [Q0JXE7-2]
DR EnsemblPlants; AT1G59640.2; AT1G59640.2; AT1G59640. [Q0JXE7-1]
DR GeneID; 842254; -.
DR Gramene; AT1G59640.1; AT1G59640.1; AT1G59640. [Q0JXE7-2]
DR Gramene; AT1G59640.2; AT1G59640.2; AT1G59640. [Q0JXE7-1]
DR KEGG; ath:AT1G59640; -.
DR Araport; AT1G59640; -.
DR TAIR; locus:2202867; AT1G59640.
DR eggNOG; ENOG502QSEM; Eukaryota.
DR InParanoid; Q0JXE7; -.
DR OMA; AFEANAM; -.
DR OrthoDB; 1369201at2759; -.
DR PhylomeDB; Q0JXE7; -.
DR PRO; PR:Q0JXE7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q0JXE7; baseline and differential.
DR Genevisible; Q0JXE7; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0048446; P:petal morphogenesis; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR024097; bHLH_ZIP_TF.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR PANTHER; PTHR12565; PTHR12565; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..343
FT /note="Transcription factor BPE"
FT /id="PRO_0000358841"
FT DOMAIN 142..192
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT VAR_SEQ 222..245
FT /note="VMILMIINSIFSIFFTKQYMFLSR -> FGQQAFENPEIQFGSQSTRE (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172,
FT ECO:0000303|PubMed:16902407, ECO:0000303|Ref.5"
FT /id="VSP_036110"
FT VAR_SEQ 250..268
FT /note="RSLDVYAVRSFKHCNKRSD -> ASPEWLHMQIGSGGFERTS (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172,
FT ECO:0000303|PubMed:16902407, ECO:0000303|Ref.5"
FT /id="VSP_036111"
FT VAR_SEQ 269..343
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172,
FT ECO:0000303|PubMed:16902407, ECO:0000303|Ref.5"
FT /id="VSP_036112"
FT CONFLICT 51
FT /note="A -> S (in Ref. 5; AAM64440)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 343 AA; 38805 MW; 811BBE39992551B6 CRC64;
MDPSGMMNEG GPFNLAEIWQ FPLNGVSTAG DSSRRSFVGP NQFGDADLTT AANGDPARMS
HALSQAVIEG ISGAWKRRED ESKSAKIVST IGASEGENKR QKIDEVCDGK AEAESLGTET
EQKKQQMEPT KDYIHVRARR GQATDSHSLA ERARREKISE RMKILQDLVP GCNKVIGKAL
VLDEIINYIQ SLQRQVEFLS MKLEAVNSRM NPGIEVFPPK EVMILMIINS IFSIFFTKQY
MFLSRYSRGR SLDVYAVRSF KHCNKRSDLC FCSCSPKTEL KTTIFSQNMT CFCRYSRVGV
AISSSKHCNE PVTLCFYSYC LRKIYHFLLW NLKYKIQKSV LFS
