T2EA_DICDI
ID T2EA_DICDI Reviewed; 456 AA.
AC Q557M8; C7FZY6; Q557J1; Q557J2; Q86AT7; Q86KI9;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 105.
DE RecName: Full=General transcription factor IIE subunit 1;
DE AltName: Full=Transcription initiation factor IIE subunit alpha;
DE Short=TFIIE-alpha;
GN Name=gtf2e1-1; ORFNames=DDB_G0273525;
GN and
GN Name=gtf2e1-2; ORFNames=DDB_G0273583;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (GTF2E1-1 AND GTF2E1-2).
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (GTF2E1-1 AND GTF2E1-2).
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Recruits TFIIH to the initiation complex and stimulates the
CC RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase
CC activities of TFIIH. Both TFIIH and TFIIE are required for promoter
CC clearance by RNA polymerase (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: TFIIE is a tetramer of two alpha and two beta subunits.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TFIIE alpha subunit family. {ECO:0000305}.
CC -!- CAUTION: The gene for this protein is duplicated in strains AX3 and
CC AX4. These strains contain a duplication of a segment of 750 kb of
CC chromosome 2 compared to the corresponding sequence in strain AX2.
CC {ECO:0000305}.
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DR EMBL; AAFI02000010; EAL70717.1; -; Genomic_DNA.
DR EMBL; AAFI02000010; EEU04133.1; -; Genomic_DNA.
DR RefSeq; XP_002649185.1; XM_002649139.1.
DR RefSeq; XP_644607.1; XM_639515.1.
DR AlphaFoldDB; Q557M8; -.
DR SMR; Q557M8; -.
DR STRING; 44689.DDB0231096; -.
DR PaxDb; Q557M8; -.
DR EnsemblProtists; EAL70717; EAL70717; DDB_G0273525.
DR EnsemblProtists; EEU04133; EEU04133; DDB_G0273583.
DR GeneID; 8618971; -.
DR GeneID; 8619006; -.
DR KEGG; ddi:DDB_G0273525; -.
DR KEGG; ddi:DDB_G0273583; -.
DR dictyBase; DDB_G0273525; gtf2e1-1.
DR dictyBase; DDB_G0273583; gtf2e1-2.
DR eggNOG; KOG2593; Eukaryota.
DR HOGENOM; CLU_600546_0_0_1; -.
DR InParanoid; Q557M8; -.
DR OMA; QEAKMDG; -.
DR PhylomeDB; Q557M8; -.
DR Reactome; R-DDI-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-DDI-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-DDI-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-DDI-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-DDI-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-DDI-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR PRO; PR:Q557M8; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005673; C:transcription factor TFIIE complex; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0001113; P:transcription open complex formation at RNA polymerase II promoter; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR039997; TFE.
DR InterPro; IPR017919; TFIIE/TFIIEa_HTH.
DR InterPro; IPR002853; TFIIE_asu.
DR InterPro; IPR024550; TFIIEa/SarR/Rpc3_HTH_dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR13097; PTHR13097; 1.
DR Pfam; PF02002; TFIIE_alpha; 1.
DR SMART; SM00531; TFIIE; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS51344; HTH_TFE_IIE; 1.
PE 3: Inferred from homology;
KW Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..456
FT /note="General transcription factor IIE subunit 1"
FT /id="PRO_0000328289"
FT DOMAIN 11..100
FT /note="HTH TFE/IIEalpha-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00676"
FT ZN_FING 129..157
FT /note="C4-type"
FT /evidence="ECO:0000255"
FT REGION 345..402
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 352..373
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 456 AA; 51466 MW; 175DDCC9673B9682 CRC64;
MSSSNNIYHI LDDLVKMVIR AFYPDEYAVI IDGLLREKKR IKDEDLALRL RIQQKYVRKI
LMDLKGDSMV KSSDVKVEAK GPNERGSTHL LWYIDYKHII DIVKYKLYMF RKKMESVKVQ
KIDVQTYKCQ TCHKVYTALD IPKLLNMDTG ALACEICDGE LEEELNNESL TQTAKHQSDL
FSQLRKIIEQ LKKTEGHNIP LFARDLADLS ADQGPSYTIN TNSSLGMGPK PSAFPVAQGA
ATSHHIDPTN ENIEFHVDIL DTDGIEINKA VVKKENKKTG LASLPPWLLP SNSFKNRNVK
SNSILNNNQQ TQTSTNEQPT AVKEQIKIDQ DFYINYIKTH YQEWESAPDS GDADGNGSNS
GSGGSTIEGN DGGNGEHQNK KMKLDDSQTV SSMSQSDDDG KDILVRVGDN LIPITKITEH
DQELMSNQEY EDYSHALYSY ASKNVFDHQY QSLMSN
