T2EA_MOUSE
ID T2EA_MOUSE Reviewed; 440 AA.
AC Q9D0D5; Q544T0;
DT 27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=General transcription factor IIE subunit 1;
DE AltName: Full=General transcription factor IIE 56 kDa subunit;
DE AltName: Full=Transcription initiation factor IIE subunit alpha;
DE Short=TFIIE-alpha;
GN Name=Gtf2e1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Recruits TFIIH to the initiation complex and stimulates the
CC RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase
CC activities of TFIIH. Both TFIIH and TFIIE are required for promoter
CC clearance by RNA polymerase.
CC -!- SUBUNIT: Tetramer of two alpha and two beta chains. Interacts with
CC TAF6/TAFII80. Interacts with ATF7IP. Interacts with SND1.
CC {ECO:0000250|UniProtKB:P29083}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P29083}.
CC -!- SIMILARITY: Belongs to the TFIIE alpha subunit family. {ECO:0000305}.
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DR EMBL; AK011543; BAB27686.1; -; mRNA.
DR EMBL; AK031528; BAC27436.1; -; mRNA.
DR EMBL; AK154283; BAE32487.1; -; mRNA.
DR EMBL; BC056966; AAH56966.1; -; mRNA.
DR CCDS; CCDS28160.1; -.
DR RefSeq; NP_083088.1; NM_028812.3.
DR RefSeq; XP_006522722.1; XM_006522659.3.
DR AlphaFoldDB; Q9D0D5; -.
DR SMR; Q9D0D5; -.
DR BioGRID; 216568; 15.
DR IntAct; Q9D0D5; 11.
DR MINT; Q9D0D5; -.
DR STRING; 10090.ENSMUSP00000023525; -.
DR iPTMnet; Q9D0D5; -.
DR PhosphoSitePlus; Q9D0D5; -.
DR EPD; Q9D0D5; -.
DR MaxQB; Q9D0D5; -.
DR PaxDb; Q9D0D5; -.
DR PeptideAtlas; Q9D0D5; -.
DR PRIDE; Q9D0D5; -.
DR ProteomicsDB; 259333; -.
DR Antibodypedia; 4082; 195 antibodies from 29 providers.
DR DNASU; 74197; -.
DR Ensembl; ENSMUST00000023525; ENSMUSP00000023525; ENSMUSG00000022828.
DR GeneID; 74197; -.
DR KEGG; mmu:74197; -.
DR UCSC; uc007zeb.1; mouse.
DR CTD; 2960; -.
DR MGI; MGI:1921447; Gtf2e1.
DR VEuPathDB; HostDB:ENSMUSG00000022828; -.
DR eggNOG; KOG2593; Eukaryota.
DR GeneTree; ENSGT00390000016696; -.
DR HOGENOM; CLU_051021_1_0_1; -.
DR InParanoid; Q9D0D5; -.
DR OMA; EYYSHMY; -.
DR OrthoDB; 1343016at2759; -.
DR PhylomeDB; Q9D0D5; -.
DR TreeFam; TF313429; -.
DR Reactome; R-MMU-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-MMU-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-MMU-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-MMU-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-MMU-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-MMU-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR BioGRID-ORCS; 74197; 15 hits in 72 CRISPR screens.
DR ChiTaRS; Gtf2e1; mouse.
DR PRO; PR:Q9D0D5; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q9D0D5; protein.
DR Bgee; ENSMUSG00000022828; Expressed in manus and 236 other tissues.
DR Genevisible; Q9D0D5; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0005669; C:transcription factor TFIID complex; ISO:MGI.
DR GO; GO:0005673; C:transcription factor TFIIE complex; IBA:GO_Central.
DR GO; GO:0097550; C:transcription preinitiation complex; ISO:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; ISO:MGI.
DR GO; GO:0006366; P:transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0001113; P:transcription open complex formation at RNA polymerase II promoter; IBA:GO_Central.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR039997; TFE.
DR InterPro; IPR017919; TFIIE/TFIIEa_HTH.
DR InterPro; IPR002853; TFIIE_asu.
DR InterPro; IPR021600; TFIIE_asu_C.
DR InterPro; IPR024550; TFIIEa/SarR/Rpc3_HTH_dom.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR013137; Znf_TFIIB.
DR PANTHER; PTHR13097; PTHR13097; 1.
DR Pfam; PF08271; TF_Zn_Ribbon; 1.
DR Pfam; PF11521; TFIIE-A_C; 1.
DR Pfam; PF02002; TFIIE_alpha; 1.
DR SMART; SM00531; TFIIE; 1.
DR PROSITE; PS51344; HTH_TFE_IIE; 1.
PE 1: Evidence at protein level;
KW Acetylation; Metal-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P29083"
FT CHAIN 2..440
FT /note="General transcription factor IIE subunit 1"
FT /id="PRO_0000211223"
FT DOMAIN 14..104
FT /note="HTH TFE/IIEalpha-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00676"
FT ZN_FING 129..157
FT /note="C4-type"
FT /evidence="ECO:0000255"
FT REGION 333..395
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:P29083"
FT MOD_RES 67
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P29083"
FT MOD_RES 268
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P29083"
SQ SEQUENCE 440 AA; 49593 MW; 0587E4DFE6FA0442 CRC64;
MADPDVLTEV PAALKRLAKY VIRGFYGIEH VLALDILIRN PCVKEEDMLE LLKFDRKQLR
SVLNNLKGDK FIKCRMRVET AADGKTTRHN YYFINYRTLV NVVKYKLDHM RRRIETDERN
STNRASFKCP VCCSTFTDLE ANQLFDPMTG TFRCTFCHTE VEEDESAMPK KDARTLLARF
NEQIEPIYAL LRETEDVNLA YEILEPEPTE IPALKQSKDR AATAAGAAGL AGGHHREAWT
NKGPSYEDLY TQNVVINMDD QDDVHRPSLE GKAAKERPIW LRESTVQGAY SSEEMKEGGI
DVDTFQEREE ARAGPDDNEE VMRALLIHEK KTSSVTAGSV GAAAPVTAAN GSDSESETSE
SDDDSPPRPA AAAPPHHHRD EDEEDEEFEE VADDPIVMVA GCPFSYSEVS QRPELVAQMT
PEEKEAYIAM GQRMFEDLFE
