ID   T2EB_DICDI              Reviewed;         276 AA.
AC   Q54KJ8;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=General transcription factor IIE subunit 2;
DE   AltName: Full=Transcription initiation factor IIE subunit beta;
DE            Short=TFIIE-beta;
GN   Name=gtf2e2; ORFNames=DDB_G0287307;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Recruits TFIIH to the initiation complex and stimulates the
CC       RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase
CC       activities of TFIIH. Both TFIIH and TFIIE are required for promoter
CC       clearance by RNA polymerase (By similarity).
CC       {ECO:0000250|UniProtKB:P29084}.
CC   -!- SUBUNIT: Tetramer of two alpha and two beta chains. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00682}.
CC   -!- SIMILARITY: Belongs to the TFIIE beta subunit family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00682}.
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DR   EMBL; AAFI02000100; EAL63721.1; -; Genomic_DNA.
DR   RefSeq; XP_637225.1; XM_632133.1.
DR   AlphaFoldDB; Q54KJ8; -.
DR   SMR; Q54KJ8; -.
DR   STRING; 44689.DDB0266450; -.
DR   PaxDb; Q54KJ8; -.
DR   EnsemblProtists; EAL63721; EAL63721; DDB_G0287307.
DR   GeneID; 8626056; -.
DR   KEGG; ddi:DDB_G0287307; -.
DR   dictyBase; DDB_G0287307; gtf2e2.
DR   eggNOG; KOG3095; Eukaryota.
DR   HOGENOM; CLU_1009826_0_0_1; -.
DR   InParanoid; Q54KJ8; -.
DR   OMA; NTDHSNG; -.
DR   PhylomeDB; Q54KJ8; -.
DR   Reactome; R-DDI-674695; RNA Polymerase II Pre-transcription Events.
DR   Reactome; R-DDI-6807505; RNA polymerase II transcribes snRNA genes.
DR   Reactome; R-DDI-73776; RNA Polymerase II Promoter Escape.
DR   Reactome; R-DDI-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR   Reactome; R-DDI-75953; RNA Polymerase II Transcription Initiation.
DR   Reactome; R-DDI-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR   PRO; PR:Q54KJ8; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0005673; C:transcription factor TFIIE complex; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IBA:GO_Central.
DR   CDD; cd07977; TFIIE_beta_winged_helix; 1.
DR   InterPro; IPR040501; TFA2_Winged_2.
DR   InterPro; IPR016656; TFIIE-bsu.
DR   InterPro; IPR003166; TFIIE_bsu_DNA-bd.
DR   PANTHER; PTHR12716; PTHR12716; 1.
DR   Pfam; PF18121; TFA2_Winged_2; 1.
DR   Pfam; PF02186; TFIIE_beta; 1.
DR   PIRSF; PIRSF016398; TFIIE-beta; 1.
DR   PROSITE; PS51351; TFIIE_BETA_C; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..276
FT                   /note="General transcription factor IIE subunit 2"
FT                   /id="PRO_0000327609"
FT   DNA_BIND        62..133
FT                   /note="TFIIE beta"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00682"
FT   REGION          256..276
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   276 AA;  31098 MW;  1F52B58E96AF309C CRC64;
     MNMAGFKETQ MKAIETLNSI KKPSSSTNSA FKYIPKISGG GGSSSANRQS SIYTANTLRD
     KQVKEKPSNR IVYDIVNYLK TLEGIPADTK EIMHSTNHMI DDKPEILELL RNNEKIIDHG
     NDRFSFKPKF NVRTQRDILD LLPNYPGGIL VSELAESYNN AESDVKKLKE TKQIFAIKAA
     ESACDIIFPN DERLRVPLSS ELVDMWKSIK LPNEADLEKE MKDAGLSVVE SVESARQTKS
     TKLKKERKKR ITKVTNTHIE NFNPNDDVPQ LGSKPI