T2EB_SCHPO
ID T2EB_SCHPO Reviewed; 285 AA.
AC P79011; Q5R228;
DT 27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 2.
DT 25-MAY-2022, entry version 131.
DE RecName: Full=Transcription initiation factor IIE subunit beta;
DE Short=TFIIE-beta;
GN Name=tfa2; ORFNames=SPCC1672.08c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND SUBUNIT.
RC STRAIN=972 / ATCC 24843;
RX PubMed=15743411; DOI=10.1111/j.1365-2443.2005.00833.x;
RA Hayashi K., Watanabe T., Tanaka A., Furumoto T., Sato-Tsuchiya C.,
RA Kimura M., Yokoi M., Ishihama A., Hanaoka F., Ohkuma Y.;
RT "Studies of Schizosaccharomyces pombe TFIIE indicate conformational and
RT functional changes in RNA polymerase II at transcription initiation.";
RL Genes Cells 10:207-224(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 62-285.
RA Kawamukai M.;
RT "S.pombe TFA2 homolog.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Recruits TFIIH to the initiation complex and stimulates the
CC RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase
CC activities of TFIIH. Both TFIIH and TFIIE are required for promoter
CC clearance by RNA polymerase (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: TFIIE is a tetramer of two alpha (tfa1) and two beta (tfa2)
CC subunits. TFIIE associates with RNA polymerase II via the beta subunit.
CC {ECO:0000269|PubMed:15743411}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TFIIE beta subunit family.
CC {ECO:0000255|PROSITE-ProRule:PRU00682}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB176673; BAD74159.1; -; mRNA.
DR EMBL; CU329672; CAA20446.1; -; Genomic_DNA.
DR EMBL; AB000475; BAA19123.1; -; mRNA.
DR PIR; T41052; T41052.
DR RefSeq; NP_587879.1; NM_001022871.2.
DR AlphaFoldDB; P79011; -.
DR SMR; P79011; -.
DR BioGRID; 275806; 18.
DR IntAct; P79011; 1.
DR STRING; 4896.SPCC1672.08c.1; -.
DR iPTMnet; P79011; -.
DR MaxQB; P79011; -.
DR PaxDb; P79011; -.
DR EnsemblFungi; SPCC1672.08c.1; SPCC1672.08c.1:pep; SPCC1672.08c.
DR GeneID; 2539236; -.
DR KEGG; spo:SPCC1672.08c; -.
DR PomBase; SPCC1672.08c; tfa2.
DR VEuPathDB; FungiDB:SPCC1672.08c; -.
DR eggNOG; KOG3095; Eukaryota.
DR HOGENOM; CLU_056580_0_1_1; -.
DR InParanoid; P79011; -.
DR OMA; MRMVFWN; -.
DR PhylomeDB; P79011; -.
DR Reactome; R-SPO-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-SPO-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-SPO-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-SPO-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-SPO-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-SPO-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR PRO; PR:P79011; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0005730; C:nucleolus; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0005673; C:transcription factor TFIIE complex; IDA:PomBase.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; TAS:PomBase.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IBA:GO_Central.
DR CDD; cd07977; TFIIE_beta_winged_helix; 1.
DR InterPro; IPR040501; TFA2_Winged_2.
DR InterPro; IPR016656; TFIIE-bsu.
DR InterPro; IPR003166; TFIIE_bsu_DNA-bd.
DR PANTHER; PTHR12716; PTHR12716; 1.
DR Pfam; PF18121; TFA2_Winged_2; 1.
DR Pfam; PF02186; TFIIE_beta; 1.
DR PIRSF; PIRSF016398; TFIIE-beta; 1.
DR PROSITE; PS51351; TFIIE_BETA_C; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..285
FT /note="Transcription initiation factor IIE subunit beta"
FT /id="PRO_0000211229"
FT DNA_BIND 67..142
FT /note="TFIIE beta"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00682"
FT REGION 1..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 240..272
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 32..56
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 254..268
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 240
FT /note="P -> A (in Ref. 1; BAD74159)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 285 AA; 32162 MW; 6FAC4EE32302733D CRC64;
MSSLSDQLSS FKKKVANQPI YAKPQPRQPA SPTPTAYLNS NDGHSSAASS PGSYSLKKKR
SKTSLVYSQP ADSGVGTHYL SQLHYAVEYL KERNEPKTAE EIASYLSTPL TPMLLNLLKK
NNRIYYDERN ETFTFKPLHN IRSGAGLLAY LDSQKTHVGM SIKELRDGWP NVTVELEELE
KQGEVLLLRT RKDGVPKMVW RNDKSCDCHV DKEFQQVWHE IPIPPTLDLA SELGKYGLKP
TSVDPSTVKR AGHNQTPKQK KPKTRRGKIT NTHLNILRDY SSMKP
