ID   T2EB_XENLA              Reviewed;         288 AA.
AC   P29540; Q5FWM0;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=General transcription factor IIE subunit 2;
DE   AltName: Full=Transcription initiation factor IIE subunit beta;
DE            Short=TFIIE-beta;
GN   Name=gtf2e2;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Oocyte;
RX   PubMed=1508726; DOI=10.1093/nar/20.16.4363;
RA   Ohkuma Y., Hashimoto S., Roeder R.G., Horikoshi M.;
RT   "Structural conservation of putative functional motifs between Xenopus and
RT   human TFIIE-beta.";
RL   Nucleic Acids Res. 20:4363-4363(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Egg;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Recruits TFIIH to the initiation complex and stimulates the
CC       RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase
CC       activities of TFIIH. Both TFIIH and TFIIE are required for promoter
CC       clearance by RNA polymerase. {ECO:0000250|UniProtKB:P29084}.
CC   -!- SUBUNIT: Tetramer of two alpha and two beta chains.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00682}.
CC   -!- SIMILARITY: Belongs to the TFIIE beta subunit family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00682}.
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DR   EMBL; Z12595; CAA78245.1; -; Genomic_DNA.
DR   EMBL; BC089287; AAH89287.1; -; mRNA.
DR   PIR; S26495; S26495.
DR   RefSeq; NP_001089256.1; NM_001095787.1.
DR   AlphaFoldDB; P29540; -.
DR   SMR; P29540; -.
DR   BioGRID; 592084; 1.
DR   IntAct; P29540; 1.
DR   PRIDE; P29540; -.
DR   DNASU; 734303; -.
DR   GeneID; 734303; -.
DR   CTD; 734303; -.
DR   Xenbase; XB-GENE-962390; gtf2e2.L.
DR   OMA; NTDHSNG; -.
DR   OrthoDB; 1160863at2759; -.
DR   Proteomes; UP000186698; Genome assembly.
DR   Bgee; 734303; Expressed in egg cell and 19 other tissues.
DR   GO; GO:0005673; C:transcription factor TFIIE complex; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IEA:InterPro.
DR   CDD; cd07977; TFIIE_beta_winged_helix; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR040501; TFA2_Winged_2.
DR   InterPro; IPR016656; TFIIE-bsu.
DR   InterPro; IPR003166; TFIIE_bsu_DNA-bd.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR12716; PTHR12716; 1.
DR   Pfam; PF18121; TFA2_Winged_2; 1.
DR   Pfam; PF02186; TFIIE_beta; 1.
DR   PIRSF; PIRSF016398; TFIIE-beta; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS51351; TFIIE_BETA_C; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..288
FT                   /note="General transcription factor IIE subunit 2"
FT                   /id="PRO_0000211228"
FT   DNA_BIND        63..143
FT                   /note="TFIIE beta"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00682"
FT   REGION          16..56
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        28..42
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   288 AA;  32624 MW;  5A3CE131641DB717 CRC64;
     MDPALLRDRE LFKKRALTTP AVEKRPSASS ESSKKKRAKL ELSSTSGSKP SSDGSNGSFN
     LKSLSGSSGY KFGVLAKIVN YMKTRHQRGD TYPLTLEEIL DETQHLDIGI KQKQWLMSEA
     LVNNPKIEII DGKYAFKPKY NLKDKKALLR LLDKHDQRGL GGILLEDIEE GLPNAQKAIK
     ALGDQIVFVT RPDKKKILFY NDKSCQFTVD EEFQKLWRSV PVDSMDDEKI EEYLKRQGIS
     SMQESGPKKI IPVQKRKKAT SQRRRFKTHN DHLAGVLKDY TDVASGKP