T2EB_YEAST
ID T2EB_YEAST Reviewed; 328 AA.
AC P36145; D6VXC3; P42569;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 3.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Transcription initiation factor IIE subunit beta;
DE Short=TFIIE-beta;
DE AltName: Full=Factor A 43 kDa subunit;
DE AltName: Full=Transcription factor A small subunit;
GN Name=TFA2; OrderedLocusNames=YKR062W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 180-194 AND 254-276,
RP AND SUBUNIT.
RC STRAIN=ATCC 208279 / BJ926;
RX PubMed=7961670; DOI=10.1016/s0021-9258(18)47019-6;
RA Feaver W.J., Henry N.L., Bushnell D.A., Sayre M.H., Brickner J.H.,
RA Gileadi O., Kornberg R.D.;
RT "Yeast TFIIE. Cloning, expression, and homology to vertebrate proteins.";
RL J. Biol. Chem. 269:27549-27553(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT "Analysis of phosphorylation sites on proteins from Saccharomyces
RT cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Recruits TFIIH to the initiation complex and stimulates the
CC RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase
CC activities of TFIIH. Both TFIIH and TFIIE are required for promoter
CC clearance by RNA polymerase (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: TFIIE is a tetramer of two alpha (TFA1) and two beta (TFA2)
CC subunits. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 1150 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the TFIIE beta subunit family.
CC {ECO:0000255|PROSITE-ProRule:PRU00682}.
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DR EMBL; U12824; AAA62664.1; -; Genomic_DNA.
DR EMBL; Z28287; CAA82141.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09213.1; -; Genomic_DNA.
DR PIR; S38138; S38138.
DR RefSeq; NP_012988.1; NM_001179852.1.
DR PDB; 5FMF; EM; 6.00 A; S=125-247.
DR PDB; 5FYW; EM; 4.35 A; X=1-328.
DR PDB; 5FZ5; EM; 8.80 A; X=1-328.
DR PDB; 5OQJ; EM; 4.70 A; X=1-328.
DR PDB; 5OQM; EM; 5.80 A; X=1-328.
DR PDB; 5SVA; EM; 15.30 A; i=1-328.
DR PDB; 6GYL; EM; 4.80 A; X=1-328.
DR PDB; 6GYM; EM; 6.70 A; X=1-328.
DR PDB; 7O4I; EM; 3.20 A; X=1-328.
DR PDB; 7O4J; EM; 2.90 A; X=1-328.
DR PDB; 7O4K; EM; 3.60 A; X=1-328.
DR PDB; 7O4L; EM; 3.40 A; X=1-328.
DR PDB; 7O72; EM; 3.40 A; X=1-328.
DR PDB; 7O73; EM; 3.40 A; X=1-328.
DR PDB; 7O75; EM; 3.20 A; X=1-328.
DR PDBsum; 5FMF; -.
DR PDBsum; 5FYW; -.
DR PDBsum; 5FZ5; -.
DR PDBsum; 5OQJ; -.
DR PDBsum; 5OQM; -.
DR PDBsum; 5SVA; -.
DR PDBsum; 6GYL; -.
DR PDBsum; 6GYM; -.
DR PDBsum; 7O4I; -.
DR PDBsum; 7O4J; -.
DR PDBsum; 7O4K; -.
DR PDBsum; 7O4L; -.
DR PDBsum; 7O72; -.
DR PDBsum; 7O73; -.
DR PDBsum; 7O75; -.
DR AlphaFoldDB; P36145; -.
DR SMR; P36145; -.
DR BioGRID; 34193; 199.
DR ComplexPortal; CPX-1658; General transcription factor complex TFIIE.
DR DIP; DIP-1701N; -.
DR IntAct; P36145; 6.
DR MINT; P36145; -.
DR STRING; 4932.YKR062W; -.
DR iPTMnet; P36145; -.
DR MaxQB; P36145; -.
DR PaxDb; P36145; -.
DR PRIDE; P36145; -.
DR EnsemblFungi; YKR062W_mRNA; YKR062W; YKR062W.
DR GeneID; 853936; -.
DR KEGG; sce:YKR062W; -.
DR SGD; S000001770; TFA2.
DR VEuPathDB; FungiDB:YKR062W; -.
DR eggNOG; KOG3095; Eukaryota.
DR GeneTree; ENSGT00390000011749; -.
DR HOGENOM; CLU_056580_2_0_1; -.
DR InParanoid; P36145; -.
DR OMA; MRMVFWN; -.
DR BioCyc; YEAST:G3O-32030-MON; -.
DR Reactome; R-SCE-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-SCE-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-SCE-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-SCE-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-SCE-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-SCE-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR PRO; PR:P36145; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P36145; protein.
DR GO; GO:0005634; C:nucleus; IDA:ComplexPortal.
DR GO; GO:0005673; C:transcription factor TFIIE complex; IDA:SGD.
DR GO; GO:0097550; C:transcription preinitiation complex; IDA:SGD.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IDA:SGD.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IDA:ComplexPortal.
DR CDD; cd07977; TFIIE_beta_winged_helix; 1.
DR InterPro; IPR040501; TFA2_Winged_2.
DR InterPro; IPR016656; TFIIE-bsu.
DR InterPro; IPR003166; TFIIE_bsu_DNA-bd.
DR PANTHER; PTHR12716; PTHR12716; 1.
DR Pfam; PF18121; TFA2_Winged_2; 1.
DR Pfam; PF02186; TFIIE_beta; 1.
DR PIRSF; PIRSF016398; TFIIE-beta; 1.
DR PROSITE; PS51351; TFIIE_BETA_C; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; DNA-binding; Nucleus;
KW Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..328
FT /note="Transcription initiation factor IIE subunit beta"
FT /id="PRO_0000211230"
FT DNA_BIND 113..187
FT /note="TFIIE beta"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00682"
FT REGION 32..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 52
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17287358"
FT MOD_RES 97
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 106
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT CONFLICT 248
FT /note="S -> R (in Ref. 1; AAA62664)"
FT /evidence="ECO:0000305"
FT STRAND 104..106
FT /evidence="ECO:0007829|PDB:7O4I"
FT HELIX 107..115
FT /evidence="ECO:0007829|PDB:7O4J"
FT HELIX 124..136
FT /evidence="ECO:0007829|PDB:7O4J"
FT HELIX 144..150
FT /evidence="ECO:0007829|PDB:7O4J"
FT HELIX 158..165
FT /evidence="ECO:0007829|PDB:7O4J"
FT STRAND 166..172
FT /evidence="ECO:0007829|PDB:7O4J"
FT TURN 173..176
FT /evidence="ECO:0007829|PDB:7O4J"
FT STRAND 177..180
FT /evidence="ECO:0007829|PDB:7O4J"
FT HELIX 189..197
FT /evidence="ECO:0007829|PDB:7O4J"
FT STRAND 200..202
FT /evidence="ECO:0007829|PDB:7O4J"
FT HELIX 207..211
FT /evidence="ECO:0007829|PDB:7O4J"
FT HELIX 218..227
FT /evidence="ECO:0007829|PDB:7O4J"
FT STRAND 229..234
FT /evidence="ECO:0007829|PDB:7O4J"
FT STRAND 236..238
FT /evidence="ECO:0007829|PDB:7O4J"
FT STRAND 241..246
FT /evidence="ECO:0007829|PDB:7O4J"
FT HELIX 257..265
FT /evidence="ECO:0007829|PDB:7O4J"
FT HELIX 271..273
FT /evidence="ECO:0007829|PDB:7O4J"
FT HELIX 274..280
FT /evidence="ECO:0007829|PDB:7O4J"
FT HELIX 290..292
FT /evidence="ECO:0007829|PDB:7O4J"
FT TURN 316..320
FT /evidence="ECO:0007829|PDB:7O4J"
SQ SEQUENCE 328 AA; 36985 MW; 8751614A7237D3E1 CRC64;
MSKNRDPLLA NLNAFKSKVK SAPVIAPAKV GQKKTNDTVI TIDGNTRKRT ASERAQENTL
NSAKNPVLVD IKKEAGSNSS NAISLDDDDD DEDFGSSPSK KVRPGSIAAA ALQANQTDIS
KSHDSSKLLW ATEYIQKKGK PVLVNELLDY LSMKKDDKVI ELLKKLDRIE FDPKKGTFKY
LSTYDVHSPS ELLKLLRSQV TFKGISCKDL KDGWPQCDET INQLEEDSKI LVLRTKKDKT
PRYVWYNSGG NLKCIDEEFV KMWENVQLPQ FAELPRKLQD LGLKPASVDP ATIKRQTKRV
EVKKKRQRKG KITNTHMTGI LKDYSHRV
