T2FA_ARATH
ID T2FA_ARATH Reviewed; 649 AA.
AC Q9SU25; F4JRD5; F4JRD6; Q6S7B9;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 126.
DE RecName: Full=Transcription initiation factor IIF subunit alpha;
DE Short=TFIIF-alpha;
DE AltName: Full=General transcription factor IIF subunit 1;
DE AltName: Full=Transcription initiation factor RAP74 homolog;
DE Short=AtRAP74;
GN Name=RAP74; Synonyms=TF2Falpha; OrderedLocusNames=At4g12610;
GN ORFNames=T1P17.200;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-640 (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Lawit S.J., Gurley W.B.;
RT "Binary protein-protein interactions of Arabidopsis thaliana general
RT transcription factors TFIIa, TFIIb, TFIId, TFIIe, and TFIIf.";
RL Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INTERACTION WITH CPL3 AND CPL4, AND SUBCELLULAR LOCATION.
RX PubMed=16905668; DOI=10.1104/pp.106.084939;
RA Bang W., Kim S., Ueda A., Vikram M., Yun D., Bressan R.A., Hasegawa P.M.,
RA Bahk J., Koiwa H.;
RT "Arabidopsis carboxyl-terminal domain phosphatase-like isoforms share
RT common catalytic and interaction domains but have distinct in planta
RT functions.";
RL Plant Physiol. 142:586-594(2006).
CC -!- FUNCTION: TFIIF is a general transcription initiation factor that binds
CC to RNA polymerase II and helps to recruit it to the initiation complex
CC in collaboration with TFIIB. It promotes transcription elongation (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer of an alpha and a beta subunit (By similarity).
CC Interacts with CPL3 and CPL4. {ECO:0000250,
CC ECO:0000269|PubMed:16905668}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16905668}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9SU25-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9SU25-2; Sequence=VSP_037404;
CC -!- SIMILARITY: Belongs to the TFIIF alpha subunit family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL049730; CAB53754.1; -; Genomic_DNA.
DR EMBL; AL161534; CAB78304.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE83155.2; -; Genomic_DNA.
DR EMBL; CP002687; AEE83156.2; -; Genomic_DNA.
DR EMBL; BX826970; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AY463611; AAR28013.1; -; mRNA.
DR PIR; D85135; D85135.
DR RefSeq; NP_001319913.1; NM_001340791.1. [Q9SU25-2]
DR RefSeq; NP_001319914.1; NM_001340792.1. [Q9SU25-1]
DR AlphaFoldDB; Q9SU25; -.
DR SMR; Q9SU25; -.
DR BioGRID; 12172; 2.
DR STRING; 3702.AT4G12610.2; -.
DR iPTMnet; Q9SU25; -.
DR PaxDb; Q9SU25; -.
DR PRIDE; Q9SU25; -.
DR ProteomicsDB; 234111; -. [Q9SU25-1]
DR EnsemblPlants; AT4G12610.1; AT4G12610.1; AT4G12610. [Q9SU25-2]
DR EnsemblPlants; AT4G12610.2; AT4G12610.2; AT4G12610. [Q9SU25-1]
DR GeneID; 826874; -.
DR Gramene; AT4G12610.1; AT4G12610.1; AT4G12610. [Q9SU25-2]
DR Gramene; AT4G12610.2; AT4G12610.2; AT4G12610. [Q9SU25-1]
DR KEGG; ath:AT4G12610; -.
DR Araport; AT4G12610; -.
DR eggNOG; KOG2393; Eukaryota.
DR HOGENOM; CLU_020656_0_0_1; -.
DR InParanoid; Q9SU25; -.
DR OMA; NNMKEFR; -.
DR OrthoDB; 662853at2759; -.
DR PhylomeDB; Q9SU25; -.
DR PRO; PR:Q9SU25; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SU25; baseline and differential.
DR GO; GO:0005674; C:transcription factor TFIIF complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0008022; F:protein C-terminus binding; IPI:UniProtKB.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; IBA:GO_Central.
DR GO; GO:0001096; F:TFIIF-class transcription factor complex binding; IBA:GO_Central.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:InterPro.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 2.
DR InterPro; IPR008851; TFIIF-alpha.
DR InterPro; IPR011039; TFIIF_interaction.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR13011; PTHR13011; 2.
DR Pfam; PF05793; TFIIF_alpha; 2.
DR SUPFAM; SSF46785; SSF46785; 2.
DR SUPFAM; SSF50916; SSF50916; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..649
FT /note="Transcription initiation factor IIF subunit alpha"
FT /id="PRO_0000376088"
FT REGION 201..480
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 466..543
FT /note="Required for CPL3 and CPL4 binding"
FT COMPBIAS 244..266
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 267..304
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 335..354
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 355..371
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 395..409
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 413..448
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 449..480
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 544..649
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_037404"
FT CONFLICT 617
FT /note="A -> T (in Ref. 4; AAR28013)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 649 AA; 72343 MW; D3F901CD82EE77F6 CRC64;
MSNCLQLNTS CVGCGSQSDL YGSSCRHMTL CLKCGRTMAQ NKSKCHECGT VVTRLIREYN
VRAAAPTDKN YFIGRFVTGL PNFKKGSENK WSLRKDIPQG RQFTDAQREK LKNKPWILED
ETGQFQYQGH LEGSQSATYY LLVMQNKEFV AIPAGSWYNF NKVAQYKQLT LEEAEEKMKN
RRKTADGYQR WMMKAANNGP ALFGEVDNEK ESGGTSGGGG RGRKKSSGGD EEEGNVSDRG
DEDEEEEASR KSRLGLNRKS NDDDDEEGPR GGDLDMDDDD IEKGDDWEHE EIFTDDDEAV
GNDPEEREDL LAPEIPAPPE IKQDEDDEEN EEEEGGLSKS GKELKKLLGK ANGLDESDED
DDDDSDDEEE TNYGTVTNSK QKEAAKEEPV DNAPAKPAPS GPPRGTPPAK PSKGKRKLND
GDSKKPSSSV QKKVKTENDP KSSLKEERAN TVSKSNTPTK AVKAEPASAP ASSSSAATGP
VTEDEIRAVL MEKKQVTTQD LVSRFKARLK TKEDKNAFAN ILRKISKIQK NAGSQNFVVL
REKCQPKPGK RESRVNKLNI RSNLQPRKME LVTEDEIRKV LMEKKQLTTL ELVMRFKERL
TTTEDKDSFS HILKKIAKLQ KNPGSEKFVV VLRDNVTPLA SDLTRLSIS
