T2FA_DICDI
ID T2FA_DICDI Reviewed; 556 AA.
AC Q54WV4;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=General transcription factor IIF subunit 1;
DE AltName: Full=Transcription initiation factor IIF subunit alpha;
DE Short=TFIIF-alpha;
GN Name=gtf2f1; ORFNames=DDB_G0279377;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: TFIIF is a general transcription initiation factor that binds
CC to RNA polymerase II and helps to recruit it to the initiation complex
CC in collaboration with TFIIB. It promotes transcription elongation (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer of an alpha and a beta subunit. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TFIIF alpha subunit family. {ECO:0000305}.
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DR EMBL; AAFI02000030; EAL67837.1; -; Genomic_DNA.
DR RefSeq; XP_641828.1; XM_636736.1.
DR AlphaFoldDB; Q54WV4; -.
DR STRING; 44689.DDB0302526; -.
DR PaxDb; Q54WV4; -.
DR PRIDE; Q54WV4; -.
DR EnsemblProtists; EAL67837; EAL67837; DDB_G0279377.
DR GeneID; 8622019; -.
DR KEGG; ddi:DDB_G0279377; -.
DR dictyBase; DDB_G0279377; gtf2f1.
DR eggNOG; KOG2393; Eukaryota.
DR HOGENOM; CLU_490421_0_0_1; -.
DR InParanoid; Q54WV4; -.
DR OMA; DGRFNAY; -.
DR Reactome; R-DDI-113418; Formation of the Early Elongation Complex.
DR Reactome; R-DDI-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-DDI-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-DDI-6803529; FGFR2 alternative splicing.
DR Reactome; R-DDI-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-DDI-72086; mRNA Capping.
DR Reactome; R-DDI-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-DDI-72165; mRNA Splicing - Minor Pathway.
DR Reactome; R-DDI-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-DDI-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-DDI-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-DDI-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-DDI-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-DDI-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR Reactome; R-DDI-9018519; Estrogen-dependent gene expression.
DR PRO; PR:Q54WV4; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005674; C:transcription factor TFIIF complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; IBA:GO_Central.
DR GO; GO:0001096; F:TFIIF-class transcription factor complex binding; IBA:GO_Central.
DR GO; GO:0032091; P:negative regulation of protein binding; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:InterPro.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IBA:GO_Central.
DR InterPro; IPR008851; TFIIF-alpha.
DR InterPro; IPR011039; TFIIF_interaction.
DR PANTHER; PTHR13011; PTHR13011; 1.
DR Pfam; PF05793; TFIIF_alpha; 1.
DR SUPFAM; SSF50916; SSF50916; 1.
PE 3: Inferred from homology;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..556
FT /note="General transcription factor IIF subunit 1"
FT /id="PRO_0000330919"
FT REGION 82..128
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 226..499
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 82..120
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 228..246
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 247..292
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 293..339
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 340..358
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 377..393
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 413..467
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 468..494
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 556 AA; 62219 MW; A9364B2C418BE6D6 CRC64;
MSESTNNPNE KTYKINLVKG TKKWNIAKFS NKIDLSSFSK PVRMYKFNPI ANMINNESNN
FGQPTTSTYT NNHYSYKNIQ PTMTSAPNGT NSTGTTPNTT TTTTTTTTTT TTTTTAAGTP
GAPNPAAATQ PFVRKKRYEA KPVNPKTIPW KLEDSEGNNT YQGNVEGNQA SSNYFLFMFQ
SDGSIKAVPC NDWYNFRPKK EFQSLTTEEA EDFMKKKNQE WDVFTSRLQK KTDPSGSGSG
GSESSGKKSI EELEEAEHRN RNEDPNRYKT TNEEKKKKKA PARRREREDE GNGEDGDAPD
FESKFDDDDD DTYMDGDGLT GEDENQIEED EEEEDVDLTE GGLEMKKMIK KQQQQNDSED
DLADDDDKKD NNGGTGGGDD EDDDEDDEDP DQDDLSNLPK VFGKSNADGT TNSSVKKEDD
GGKDSKSSKK SKKKDKDSSP KSKEKKSKKN KSDSSVDNRE SKKIKKEPSS PQAVQPNSPS
QQQQQQQQNI DPNDPPFTEE YIKIVLQKSK KVKSLDLINI FKGPLKNPDN KPIFLAMVHN
VARVVEENGV KYLIPK