BPH1_YEAST
ID BPH1_YEAST Reviewed; 2167 AA.
AC P25356; D6VR42; Q02396; Q07348;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 27-JUN-2003, sequence version 2.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Beige protein homolog 1;
GN Name=BPH1; OrderedLocusNames=YCR032W; ORFNames=YCR32W, YCR591, YCR601;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204511 / S288c / AB972;
RX PubMed=1837415; DOI=10.1002/yea.320070612;
RA Rodriguez F., Martegani E., Mauri I., Alberghina L.;
RT "The sequence of 8.8 kb of yeast chromosome III cloned in lambda PM3270
RT contains an unusual long ORF (YCR601).";
RL Yeast 7:631-641(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=1574125; DOI=10.1038/357038a0;
RA Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M.,
RA Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G.,
RA Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A.,
RA Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M., Carcano C.,
RA Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M.,
RA Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C.,
RA Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F.,
RA Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C.,
RA Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E.,
RA Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P.,
RA Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J.,
RA Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P.,
RA Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M.,
RA Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P.,
RA Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G.,
RA Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E.,
RA Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F.,
RA Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L.,
RA Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J.,
RA Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M.,
RA Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A.,
RA Richterich P., Roberts A.B., Rodriguez F., Sanz E.,
RA Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J.,
RA Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I.,
RA Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M.,
RA Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M.,
RA Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D.,
RA Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K.,
RA Sgouros J.G.;
RT "The complete DNA sequence of yeast chromosome III.";
RL Nature 357:38-46(1992).
RN [3]
RP SEQUENCE REVISION.
RA Valles G., Volckaerts G.;
RL Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 335-2167.
RX PubMed=1872032; DOI=10.1002/yea.320070411;
RA Jia Y., Slonimski P.P., Herbert C.J.;
RT "The complete sequence of the unit YCR59, situated between CRY1 and MAT,
RT reveals two long open reading frames, which cover 91% of the 10.1 kb
RT segment.";
RL Yeast 7:413-424(1991).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1760-2167.
RX PubMed=1776366; DOI=10.1002/yea.320070711;
RA Wicksteed B.L., Roberts A.B., Sagliocco F.A., Brown A.J.P.;
RT "The complete sequence of a 7.5 kb region of chromosome III from
RT Saccharomyces cerevisiae that lies between CRY1 and MAT.";
RL Yeast 7:761-772(1991).
RN [7]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15296494; DOI=10.1111/j.1600-0854.2004.00213.x;
RA Shiflett S.L., Vaughn M.B., Huynh D., Kaplan J., McVey Ward D.;
RT "Bph1p, the Saccharomyces cerevisiae homologue of CHS1/beige, functions in
RT cell wall formation and protein sorting.";
RL Traffic 5:700-710(2004).
RN [8]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-1667, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22106047; DOI=10.1002/pmic.201100166;
RA Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.;
RT "Sites of ubiquitin attachment in Saccharomyces cerevisiae.";
RL Proteomics 12:236-240(2012).
CC -!- FUNCTION: May be involved in protein sorting and cell wall formation.
CC {ECO:0000269|PubMed:15296494}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15296494}. Membrane
CC {ECO:0000269|PubMed:15296494}; Peripheral membrane protein
CC {ECO:0000269|PubMed:15296494}; Cytoplasmic side
CC {ECO:0000269|PubMed:15296494}.
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DR EMBL; X62452; CAA44309.1; -; Genomic_DNA.
DR EMBL; X59720; CAC42982.1; -; Genomic_DNA.
DR EMBL; X59075; CAA41798.1; -; Genomic_DNA.
DR EMBL; S78624; AAB21258.1; -; Genomic_DNA.
DR EMBL; BK006937; DAA07511.1; -; Genomic_DNA.
DR PIR; S19444; S19444.
DR RefSeq; NP_009961.2; NM_001178746.1.
DR AlphaFoldDB; P25356; -.
DR SMR; P25356; -.
DR BioGRID; 31015; 132.
DR MINT; P25356; -.
DR STRING; 4932.YCR032W; -.
DR iPTMnet; P25356; -.
DR MaxQB; P25356; -.
DR PaxDb; P25356; -.
DR PRIDE; P25356; -.
DR EnsemblFungi; YCR032W_mRNA; YCR032W; YCR032W.
DR GeneID; 850398; -.
DR KEGG; sce:YCR032W; -.
DR SGD; S000000628; BPH1.
DR VEuPathDB; FungiDB:YCR032W; -.
DR eggNOG; KOG1786; Eukaryota.
DR HOGENOM; CLU_000175_3_1_1; -.
DR InParanoid; P25356; -.
DR OMA; IRFLFFQ; -.
DR BioCyc; YEAST:G3O-29346-MON; -.
DR PRO; PR:P25356; -.
DR Proteomes; UP000002311; Chromosome III.
DR RNAct; P25356; protein.
DR GO; GO:0005829; C:cytosol; IDA:SGD.
DR GO; GO:0019898; C:extrinsic component of membrane; IDA:SGD.
DR GO; GO:0000329; C:fungal-type vacuole membrane; HDA:SGD.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0019901; F:protein kinase binding; IBA:GO_Central.
DR GO; GO:0031505; P:fungal-type cell wall organization; IMP:SGD.
DR GO; GO:0006886; P:intracellular protein transport; IMP:SGD.
DR GO; GO:0008104; P:protein localization; IBA:GO_Central.
DR GO; GO:0009268; P:response to pH; IMP:SGD.
DR CDD; cd06071; Beach; 1.
DR CDD; cd01201; PH_BEACH; 1.
DR Gene3D; 1.10.1540.10; -; 1.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR000409; BEACH_dom.
DR InterPro; IPR036372; BEACH_dom_sf.
DR InterPro; IPR023362; PH-BEACH_dom.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF02138; Beach; 1.
DR Pfam; PF14844; PH_BEACH; 1.
DR SMART; SM01026; Beach; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF81837; SSF81837; 1.
DR PROSITE; PS50197; BEACH; 1.
DR PROSITE; PS51783; PH_BEACH; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Isopeptide bond; Membrane; Reference proteome; Repeat;
KW Ubl conjugation; WD repeat.
FT CHAIN 1..2167
FT /note="Beige protein homolog 1"
FT /id="PRO_0000050887"
FT DOMAIN 1368..1499
FT /note="BEACH-type PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01119"
FT DOMAIN 1545..1839
FT /note="BEACH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00026"
FT REPEAT 1927..1965
FT /note="WD 1"
FT REPEAT 1976..2015
FT /note="WD 2"
FT REPEAT 2017..2054
FT /note="WD 3"
FT REPEAT 2072..2111
FT /note="WD 4"
FT REPEAT 2129..2167
FT /note="WD 5"
FT CROSSLNK 1667
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0007744|PubMed:22106047"
FT CONFLICT 1893
FT /note="F -> N (in Ref. 6; AAB21258)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2167 AA; 250872 MW; ED7D607ADA9982CD CRC64;
MNSIINAASK VLRLQDDVKK ATIILGDILI LQPINHEVEP DVENLVQHEL TKIIQGYPIQ
DNMIINSKKG TVEDDLCELN NYTCFALSKS FDLCHDSRNF NIAQPKRWIQ LLETLTDSVS
FAVIVQIILT LSNISLINKQ TLGKLKKLRI RIFEILSNKN DSWKSTLLQK NLIEWYIFML
SVDCTPLELQ NLYLHKELKF CNGILNSLTL QVSDPRSQNY LQFENTYKLF QIQKSSRINN
SFLFYIEFNS VTSNRIMTIE RHIYLEIKEG QFCISNDNYI IGLFENFEFE AGTLYFIGVL
IDHNNRITLY VDGSMINQLT LFENSICQLS TCELGSMICS IKVYRFYLWD GLLTEFAINI
LQAIGTNYQY TFSKKKEGPE VLSLCQDFLI AKAHLMARPT TEISSTKYID EIELLEMENI
IIDVNPNDIL QDFTESSNFT VKFEESTNSK NIPEVGKCYF YRSSNLVSKF VSIDSIRLAF
LNMTESGSID DLFHHVSHLM NLLRNIDILN WFKKDFGFPL FAYTLKQKIT QDLSQPLNIQ
FFNLFLEFCG WDFNDISKSI ILDTDAYENI VLNLDLWYMN EDQSSLASGG LEIIRFLFFQ
ISSLMEASIY SKFNSNKFND MNILEKLCLS YQAVTKRENQ NSKFNELSSD LISVFVTLLK
SNTDKRHLQW FLHLSYYFIK RKDVRSTEII LQAVDQLFSF YLDQGSDENA KILSEIIPLK
LILMIMDQIV ENNESNPITC LNILFKVVLT NKPLFKQFYK NDGLKLILTM LCKVGKSYRE
EIISLLLTYS IGNYTTANEI FSGAEDMIGG ISNDKITAKE IIYLAVNFIE WHVINSNASD
SSSVLDLNNH ILRFVEDLKS LSAVPINESV FDPKKSYVMV SLLDLSIALN ESEDISKFKS
SSKVISELIK GNIMCALTKY AAYDFEVYMS TFFCHSTEYK LVYPKTVMNN SSYLELSFIV
TLLPEILNDL VDSNNNLNLM MLKHPYTMSN LLYFLRKFRP DTSQIVMPKD FYFSSYTCLL
HCVIQIDKSS FYHFKNVSKS QLLQDFKICI MNLIYSNTLK QIIWEKEEYE MFSESLMAHQ
EVLFAHGACD NETVGLLLIF FANRLRDCGY NKAVFNCMKV IIKNKERKLK EVACFFDPAN
KSEVLEGLSN ILSCNNSETM NLITEQYPFF FNNTQQVRFI NIVTNILFKN NNFSPISVRQ
IKNQVYEWKN ARSEYVTQNN KKCLILFRKD NTSLDFKIKK SISRYTYNLK TDREENAVFY
RNNLNLLIFH LKHTLEIQSN PNSSCKWSSD FAEDFDGMKR RLLPAWEPKY EPLINEEDAN
QDTITGGNRQ RRESGSILSY EFIEHMETLE SEPVGDLNEN RKILRLLKDN DSIATIWNCS
LIIGLEIKEG ILIHGSNYLY FVSDYYFSLE DKKILKLSEV SQESRDMTVS LINGPDVKRV
STFLKHEVFV WKLLDITFVT KRPFLLRDVA IELLFKERVS AFFSFYNKRV RDDVLRVLNK
IPKHLPADPI FSSVLQEIND RGNSIVARNG IGKASIASKF TSVFSANNSL IDGFEISKKW
VRGEISNFYY LLSINILAGR SFNDLTQYPV FPWVIADYES NVLDLENPKT YRDLSKPMGA
QSEKRKLQFI ERYEALASLE NADSAPFHYG THYSSAMIVS SYLIRLKPFV ESFLLLQGGS
FGPADRLFSS LERAWSSASS ENTTDVRELT PEFFFLPEFL INVNSYDFGT DQSGKKVDDV
VLPPWANGDP KVFIQKNREA LESPYVSAHL HEWIDLIFGY KQKGDIAVKS VNVFNRLSYP
GAVNLDNIDD ENERRAITGI IHNFGQTPLQ IFQEPHPEKI ACNVQQLTTE VWRKVPMKPI
FEKTIFNLNE KNRSVDYVIH DPSYFDSLYW RGFAFPNLFF RTEESLVSLR IVHKNWLKIG
LDIFKKTHMA QITSFAYWKL GEFITGDKNG LIKVWKYRKD KHSVSGNLEN KKTMFGHLCE
LKEMRCYHDY NTLLTLDISG LVYVWDMINF ELVRQITNDA QKVAISQHAG SIMVLTKNNA
ISIFNLNGQI YTSKKFEPAK IVSSIDFFDF TKLDAGYRKH IYWKEMEILL VGFEDGTIEI
YELFLNFHNE WAIKLLKQLC TEKGKAITSI KGQGKTYLSQ KRRKDTAEPH EIEVIAGTLD
GRLAIWY
