T2FB_MOUSE
ID T2FB_MOUSE Reviewed; 249 AA.
AC Q8R0A0;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=General transcription factor IIF subunit 2;
DE AltName: Full=Transcription initiation factor IIF subunit beta;
DE Short=TFIIF-beta;
GN Name=Gtf2f2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP INTERACTION WITH GPBP1.
RX PubMed=14612417; DOI=10.1128/mcb.23.23.8773-8785.2003;
RA Hsu L.-C., Liu S., Abedinpour F., Beech R.D., Lahti J.M., Kidd V.J.,
RA Greenspan J.A., Yeung C.-Y.;
RT "The murine G+C-rich promoter binding protein mGPBP is required for
RT promoter-specific transcription.";
RL Mol. Cell. Biol. 23:8773-8785(2003).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Liver, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-137, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT pathways.";
RL Mol. Cell 50:919-930(2013).
CC -!- FUNCTION: TFIIF is a general transcription initiation factor that binds
CC to RNA polymerase II and helps to recruit it to the initiation complex
CC in collaboration with TFIIB. {ECO:0000250|UniProtKB:P13984}.
CC -!- SUBUNIT: Heterodimer of an alpha and a beta subunit. Interacts with
CC HTATSF1 and URI1 (By similarity). Interacts with GPBP1. Interacts with
CC GTF2B (via N-terminus); this interaction is inhibited in presence of
CC GTF2F1 (By similarity). {ECO:0000250, ECO:0000250|UniProtKB:P13984,
CC ECO:0000269|PubMed:14612417}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TFIIF beta subunit family. {ECO:0000305}.
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DR EMBL; BC027173; AAH27173.1; -; mRNA.
DR CCDS; CCDS36981.1; -.
DR RefSeq; NP_081092.1; NM_026816.3.
DR AlphaFoldDB; Q8R0A0; -.
DR SMR; Q8R0A0; -.
DR BioGRID; 213003; 5.
DR ComplexPortal; CPX-83; Transcription factor TFIIF complex.
DR STRING; 10090.ENSMUSP00000086312; -.
DR iPTMnet; Q8R0A0; -.
DR PhosphoSitePlus; Q8R0A0; -.
DR EPD; Q8R0A0; -.
DR MaxQB; Q8R0A0; -.
DR PaxDb; Q8R0A0; -.
DR PRIDE; Q8R0A0; -.
DR ProteomicsDB; 263201; -.
DR Antibodypedia; 1838; 281 antibodies from 30 providers.
DR DNASU; 68705; -.
DR Ensembl; ENSMUST00000088922; ENSMUSP00000086312; ENSMUSG00000067995.
DR GeneID; 68705; -.
DR KEGG; mmu:68705; -.
DR UCSC; uc007urb.1; mouse.
DR CTD; 2963; -.
DR MGI; MGI:1915955; Gtf2f2.
DR VEuPathDB; HostDB:ENSMUSG00000067995; -.
DR eggNOG; KOG2905; Eukaryota.
DR GeneTree; ENSGT00390000016051; -.
DR HOGENOM; CLU_047858_1_0_1; -.
DR InParanoid; Q8R0A0; -.
DR OMA; KYIANKW; -.
DR OrthoDB; 1496393at2759; -.
DR PhylomeDB; Q8R0A0; -.
DR TreeFam; TF314290; -.
DR Reactome; R-MMU-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-MMU-113418; Formation of the Early Elongation Complex.
DR Reactome; R-MMU-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-MMU-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-MMU-6803529; FGFR2 alternative splicing.
DR Reactome; R-MMU-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-MMU-72086; mRNA Capping.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-MMU-72165; mRNA Splicing - Minor Pathway.
DR Reactome; R-MMU-72203; Processing of Capped Intron-Containing Pre-mRNA.
DR Reactome; R-MMU-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-MMU-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-MMU-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-MMU-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-MMU-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-MMU-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR Reactome; R-MMU-9018519; Estrogen-dependent gene expression.
DR BioGRID-ORCS; 68705; 21 hits in 77 CRISPR screens.
DR ChiTaRS; Gtf2f2; mouse.
DR PRO; PR:Q8R0A0; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q8R0A0; protein.
DR Bgee; ENSMUSG00000067995; Expressed in floor plate of midbrain and 259 other tissues.
DR ExpressionAtlas; Q8R0A0; baseline and differential.
DR Genevisible; Q8R0A0; MM.
DR GO; GO:0015630; C:microtubule cytoskeleton; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0005674; C:transcription factor TFIIF complex; ISO:MGI.
DR GO; GO:0005675; C:transcription factor TFIIH holo complex; ISO:MGI.
DR GO; GO:0097550; C:transcription preinitiation complex; ISO:MGI.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; ISO:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; ISO:MGI.
DR GO; GO:0006366; P:transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; ISO:MGI.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; ISO:MGI.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR003196; TFIIF_beta.
DR InterPro; IPR040450; TFIIF_beta_HTH.
DR InterPro; IPR040504; TFIIF_beta_N.
DR InterPro; IPR011039; TFIIF_interaction.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR10445; PTHR10445; 1.
DR Pfam; PF02270; TFIIF_beta; 1.
DR Pfam; PF17683; TFIIF_beta_N; 1.
DR PIRSF; PIRSF015849; TFIIF-beta; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF50916; SSF50916; 1.
PE 1: Evidence at protein level;
KW Acetylation; DNA-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P13984"
FT CHAIN 2..249
FT /note="General transcription factor IIF subunit 2"
FT /id="PRO_0000211236"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:P13984"
FT MOD_RES 22
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P13984"
FT MOD_RES 33
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P13984"
FT MOD_RES 137
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
FT MOD_RES 142
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P13984"
FT MOD_RES 248
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P13984"
SQ SEQUENCE 249 AA; 28381 MW; E125CD762A42EB14 CRC64;
MAERGELDLT GAKQNTGVWL VKVPKYLSQQ WAKASGRGEV GKLRIAKNQG RTEVSFTLNE
DLANIHDIGG KPASVSAPRE HPFVLQSVGG QTLTVFTESS SDKLSLEGIV VQRAECRPAA
SENYMKLKRL QIEESSKPVR LSQQLDKVVT TNYKPVANHQ YNIEYERKKK EDGKRARADK
QHVLDMLFSA FEKHQYYNLK DLVDITKQPV GYLKEILKEI GIQNVKGIHK NTWELKPEYR
HYQTEEKSD
