T2FB_RAT
ID T2FB_RAT Reviewed; 249 AA.
AC Q01750;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=General transcription factor IIF subunit 2;
DE AltName: Full=Transcription initiation factor IIF subunit beta;
DE Short=TFIIF-beta;
DE AltName: Full=Transcription initiation factor RAP30;
GN Name=Gtf2f2; Synonyms=Rap30;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1429731; DOI=10.1016/s0021-9258(18)35928-3;
RA Garrett K.P., Serizawa H., Hanley J.P., Bradsher J.N., Tsuboi A., Arai N.,
RA Yokota T., Arai K., Conaway R.C., Conaway J.W.;
RT "The carboxyl terminus of RAP30 is similar in sequence to region 4 of
RT bacterial sigma factors and is required for function.";
RL J. Biol. Chem. 267:23942-23949(1992).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-248, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: TFIIF is a general transcription initiation factor that binds
CC to RNA polymerase II and helps to recruit it to the initiation complex
CC in collaboration with TFIIB. It promotes transcription elongation.
CC {ECO:0000250|UniProtKB:P13984}.
CC -!- SUBUNIT: Heterodimer of an alpha and a beta subunit. Interacts with
CC HTATSF1, GPBP1 and URI1 (By similarity). Interacts with GTF2B (via N-
CC terminus); this interaction is inhibited in presence of GTF2F1 (By
CC similarity). {ECO:0000250|UniProtKB:P13984}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the TFIIF beta subunit family. {ECO:0000305}.
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DR EMBL; L01267; AAA42005.1; -; mRNA.
DR AlphaFoldDB; Q01750; -.
DR SMR; Q01750; -.
DR STRING; 10116.ENSRNOP00000044668; -.
DR iPTMnet; Q01750; -.
DR PhosphoSitePlus; Q01750; -.
DR PaxDb; Q01750; -.
DR PRIDE; Q01750; -.
DR UCSC; RGD:620772; rat.
DR RGD; 620772; Gtf2f2.
DR eggNOG; KOG2905; Eukaryota.
DR InParanoid; Q01750; -.
DR PhylomeDB; Q01750; -.
DR Reactome; R-RNO-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-RNO-113418; Formation of the Early Elongation Complex.
DR Reactome; R-RNO-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-RNO-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-RNO-6803529; FGFR2 alternative splicing.
DR Reactome; R-RNO-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-RNO-72086; mRNA Capping.
DR Reactome; R-RNO-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-RNO-72165; mRNA Splicing - Minor Pathway.
DR Reactome; R-RNO-72203; Processing of Capped Intron-Containing Pre-mRNA.
DR Reactome; R-RNO-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-RNO-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-RNO-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-RNO-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-RNO-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-RNO-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR Reactome; R-RNO-9018519; Estrogen-dependent gene expression.
DR PRO; PR:Q01750; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005634; C:nucleus; ISO:RGD.
DR GO; GO:0005674; C:transcription factor TFIIF complex; ISO:RGD.
DR GO; GO:0005675; C:transcription factor TFIIH holo complex; IDA:RGD.
DR GO; GO:0097550; C:transcription preinitiation complex; ISO:RGD.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; IDA:ARUK-UCL.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IDA:RGD.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IDA:RGD.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IDA:RGD.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IDA:ARUK-UCL.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR003196; TFIIF_beta.
DR InterPro; IPR040450; TFIIF_beta_HTH.
DR InterPro; IPR040504; TFIIF_beta_N.
DR InterPro; IPR011039; TFIIF_interaction.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR10445; PTHR10445; 1.
DR Pfam; PF02270; TFIIF_beta; 1.
DR Pfam; PF17683; TFIIF_beta_N; 1.
DR PIRSF; PIRSF015849; TFIIF-beta; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF50916; SSF50916; 1.
PE 1: Evidence at protein level;
KW Acetylation; DNA-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P13984"
FT CHAIN 2..249
FT /note="General transcription factor IIF subunit 2"
FT /id="PRO_0000211237"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:P13984"
FT MOD_RES 22
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P13984"
FT MOD_RES 33
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P13984"
FT MOD_RES 137
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P13984"
FT MOD_RES 142
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P13984"
FT MOD_RES 248
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
SQ SEQUENCE 249 AA; 28349 MW; 5299CAD9AF454A91 CRC64;
MAERGELDLT GAKQNTGVWL VKVPKYLSQQ WAKAPGRGEV GKLRIAKNQG RTEVSFTLNE
DLANIHDIGG KPASVSAPRE HPFVLQSVGG QTLTVFTESS SDKLSLEGIV VQRAECRPAA
SENYMKLKRL QIEESSKPVR LSQQADKVVT TNYKPVANHQ YNIEYERKKK EDGKRARADK
QHVLDMLFSA FEKHQYYNLK DLVDITKQPV GYLKEILKEI GIQNVKGIHK NTWELKPEYR
HYQTEEKSD
