T2FB_SCHPO
ID T2FB_SCHPO Reviewed; 307 AA.
AC O94424;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Transcription initiation factor IIF subunit beta;
DE AltName: Full=TFIIF medium subunit;
DE AltName: Full=TFIIF-beta;
GN Name=tfg2; ORFNames=SPBC1198.13c, SPBC660.03c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP INTERACTION WITH TFG3.
RX PubMed=15616156; DOI=10.1093/nar/gkh1000;
RA Kimura M., Ishihama A.;
RT "Tfg3, a subunit of the general transcription factor TFIIF in
RT Schizosaccharomyces pombe, functions under stress conditions.";
RL Nucleic Acids Res. 32:6706-6715(2004).
CC -!- FUNCTION: TFIIF is a general transcription initiation factor that binds
CC to RNA polymerase II and helps to recruit it to the initiation complex
CC in collaboration with TFIIB. It promotes transcription elongation (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the fcp1/TFIIF/polII complex via interaction of
CC tfg3 with both tfg1/TFIIF-alpha and tfg2/TFIIF-beta subunits.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TFIIF beta subunit family. {ECO:0000305}.
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DR EMBL; CU329671; CAB91188.1; -; Genomic_DNA.
DR PIR; T40615; T40615.
DR RefSeq; NP_595082.1; NM_001020988.2.
DR AlphaFoldDB; O94424; -.
DR SMR; O94424; -.
DR BioGRID; 276468; 7.
DR IntAct; O94424; 1.
DR STRING; 4896.SPBC1198.13c.1; -.
DR iPTMnet; O94424; -.
DR MaxQB; O94424; -.
DR PaxDb; O94424; -.
DR PRIDE; O94424; -.
DR EnsemblFungi; SPBC1198.13c.1; SPBC1198.13c.1:pep; SPBC1198.13c.
DR GeneID; 2539924; -.
DR KEGG; spo:SPBC1198.13c; -.
DR PomBase; SPBC1198.13c; tfg2.
DR VEuPathDB; FungiDB:SPBC1198.13c; -.
DR eggNOG; KOG2905; Eukaryota.
DR HOGENOM; CLU_047858_0_0_1; -.
DR InParanoid; O94424; -.
DR OMA; YMKVKRL; -.
DR PhylomeDB; O94424; -.
DR Reactome; R-SPO-113418; Formation of the Early Elongation Complex.
DR Reactome; R-SPO-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-SPO-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-SPO-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-SPO-72086; mRNA Capping.
DR Reactome; R-SPO-72165; mRNA Splicing - Minor Pathway.
DR Reactome; R-SPO-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-SPO-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-SPO-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-SPO-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-SPO-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-SPO-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR Reactome; R-SPO-9018519; Estrogen-dependent gene expression.
DR PRO; PR:O94424; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0005674; C:transcription factor TFIIF complex; ISS:PomBase.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; ISS:PomBase.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; ISS:PomBase.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR003196; TFIIF_beta.
DR InterPro; IPR040450; TFIIF_beta_HTH.
DR InterPro; IPR040504; TFIIF_beta_N.
DR InterPro; IPR011039; TFIIF_interaction.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR10445; PTHR10445; 1.
DR Pfam; PF02270; TFIIF_beta; 1.
DR Pfam; PF17683; TFIIF_beta_N; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF50916; SSF50916; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleotide-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..307
FT /note="Transcription initiation factor IIF subunit beta"
FT /id="PRO_0000238605"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 261..307
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 261..289
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 290..307
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 307 AA; 34623 MW; 23CF468C3F81E101 CRC64;
MSEEKPTVRT EEDDRYEDDA GDLDLGQIGS RVWLVKIPKF LMDKWNSIPE DDAANLGCVR
VKNDEIQLLL QNSPENADVP KIYNLRVMNK FVRNSYVFRE SETSSSMKST ALVGTVAHEC
NVSPVINDDY RRVMQKRALA ASAPKRKVQM IDDRGGSLLA PGTLGSRSRS TTSFIRNVKP
RTGEGLKNSR IPRNELLDIL FKCFEDYEYW TLKGLREYVK QPEVYLKEVL DSIAILNKRG
PYALKYSLKP EYKGTMDAAS VELRNQQASQ SESSSIDHTG KNTSPDNPGT NAEEDEDDDG
VEMIDVV
